cDNA Clone PACT

• Plasmid: pEGFP-C2-TAP PACT
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-PACT
• Genbank: AF072860
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCCAG
AGCAGGCACCGCGCCGAGGCCCCGCCGCTGGAGCGCGAGGACAGTGGGAC
CTTCAGTTTGGGGAAGATGATAACAGCTAAGCCAGGGAAAACACCGATTC
AGGTATTACACGAATACGGCATGAAGACCAAGAACATCCCAGTTTATGAA
TGTGAAAGATCTGATGTGCAAATACACGTGCCCACTTTCACCTTCAGAGT
AACCGTTGGTGACATAACCTGCACAGGTGAAGGTACAAGTAAGAAGCTGG
CGAAACATAGAGCTGCAGAGGCTGCCATAAACATTTTGAAAGCCAATGCA
AGTATTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGACCCTTCCAAGCA
ACCAAAGAACCAGCTTAATCCTATTGGTTCATTACAGGAATTGGCTATTC
ATCATGGCTGGAGACTTCCTGAATATACCCTTTCCCAGGAGGGAGGACCT
GCTCATAAGAGAGAATATACTACAATTTGCAGGCTAGAGTCATTTATGGA
AACTGGAAAGGGGGCATCAAAAAAGCAAGCCAAAAGGAATGCTGCTGAGA
AATTTCTTGCCAAATTTAGTAATATTTCTCCAGAGAACCACATTTCTTTA
ACAAATGTAGTAGGACATTCTTTAGGATGTACTTGGCATTCCTTGAGGAA
TTCTCCTGGTGAAAAGATCAACTTACTGAAAAGAAGCCTCCTTAGTATTC
CAAATACAGATTACATCCAGCTGCTTAGTGAAATTGCCAAGGAACAAGGT
TTTAATATAACATATTTGGATATAGATGAACTGAGCGCCAATGGACAATA
TCAATGTCTTGCTGAACTGTCCACCAGCCCCATCACAGTCTGTCATGGCT
CCGGTATCTCCTGTGGCAATGCACAAAGTGATGCAGCTCACAATGCTTTG
CAGTATTTAAAGATAATAGCAGAAAGAAAGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMSQ
SRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYE
CERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANA
SICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGP
AHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISL
TNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQG
FNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNAL
QYLKIIAERK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GFP.