cDNA Clone EIF2C4

• Plasmid: pEGFP-C2-TAP EIF2C4
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-EIF2C4
• Genbank: NM_017629
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCAGATCTGGT
ACCATGGAGGCGCTGGGACCCGGACCTCCGGCTAGCCTGTTTCAGCCACC
TCGTCGTCCTGGCCTTGGAACTGTTGGAAAACCAATTCGACTGTTAGCCA
ATCATTTTCAGGTTCAGATTCCTAAAATAGATGTGTATCACTATGATGTG
GATATTAAGCCTGAAAAACGGCCTCGTAGAGTCAACAGGGAGGTAGTAGA
TACAATGGTGCGGCACTTCAAGATGCAAATATTTGGTGATCGGCAGCCTG
GGTATGATGGCAAAAGAAACATGTACACAGCACATCCACTACCAATTGGA
CGGGATAGGGTTGATATGGAGGTGACTCTTCCAGGCGAGGGTAAAGACCA
AACATTTAAAGTGTCTGTTCAGTGGGTGTCAGTTGTGAGCCTTCAGTTGC
TTTTAGAAGCTTTGGCTGGGCACTTGAATGAAGTCCCAGATGACTCAGTA
CAAGCACTTGATGTTATCACAAGACACCTTCCCTCCATGAGGTACACCCC
AGTGGGCCGTTCCTTTTTCTCACCCCCGGAAGGTTACTACCACCCTCTGG
GAGGGGGCAGGGAGGTCTGGTTTGGTTTTCATCAGTCTGTGAGACCTGCC
ATGTGGAATATGATGCTCAACATTGATGTATCTGCAACTGCTTTCTACCG
GGCTCAGCCTATCATTGAGTTCATGTGTGAGGTTTTAGACATTCAGAACA
TCAATGAACAGACCAAACCTCTAACAGACTCCCAGCGTGTCAAATTTACC
AAAGAAATCAGAGGTCTCAAAGTTGAGGTGACCCACTGTGGACAGATGAA
ACGAAAATACCGAGTTTGTAATGTGACTAGACGGCCAGCCAGTCATCAAA
CTTTTCCTTTGCAGCTAGAAAACGGTCAAGCTATGGAATGTACAGTAGCT
CAATATTTTAAGCAAAAGTATAGTCTGCAACTGAAATACCCCCATCTTCC
CTGTCTCCAAGTGGGACAAGAACAAAAGCATACATACTTGCCACTCGAGG
TCTGTAATATAGTGGCAGGACAGCGATGTATCAAGAAGCTCACAGACAAT
CAGACTTCCACAATGATCAAAGCTACAGCAAGATCTGCTCCTGACAGACA
GGAAGAGATCAGTAGACTGGTGAAGAGCAACAGTATGGTGGGTGGACCTG
ATCCATACCTTAAAGAATTTGGTATTGTTGTCCACAATGAAATGACAGAG
CTCACAGGCAGGGTACTTCCAGCACCAATGCTGCAATATGGAGGCCGGAA
TAAAACAGTAGCCACACCCAACCAGGGTGTCTGGGACATGCGAGGAAAGC
AGTTTTATGCTGGCATTGAAATTAAAGTTTGGGCAGTTGCTTGTTTTGCA
CCTCAGAAACAATGTAGGGAAGATTTACTAAAGAGTTTCACTGACCAGCT
GCGTAAAATCTCTAAGGATGCAGGAATGCCCATCCAGGGTCAGCCATGTT
TCTGCAAGTATGCACAAGGTGCAGACAGTGTGGAGCCTATGTTTAAACAT
CTGAAAATGACTTATGTGGGCCTACAGCTAATAGTGGTTATCCTGCCTGG
AAAGACACCAGTATATGCGGAGGTGAAACGTGTTGGAGATACCCTTCTAG
GTATGGCCACACAGTGTGTCCAGGTAAAAAATGTAGTGAAGACCTCACCT
CAAACCCTTTCCAATCTTTGCCTGAAGATAAATGCAAAACTTGGAGGAAT
TAACAATGTGCTTGTGCCTCATCAAAGGCCCTCGGTGTTCCAGCAGCCTG
TCATCTTCCTGGGAGCGGATGTCACACACCCCCCAGCAGGGGATGGGAAG
AAACCTTCCATTGCTGCTGTGGTTGGCAGTATGGATGGCCACCCCAGCCG
GTACTGTGCCACCGTTCGGGTGCAGACTTCCCGGCAGGAGATCTCCCAAG
AGCTCCTCTACAGTCAAGAGGTCATCCAGGACCTGACTAACATGGTTCGA
GAGCTGCTGATTCAGTTCTACAAATCCACACGCTTCAAACCCACTCGGAT
CATCTATTACCGTGGAGGGGTATCTGAGGGACAAATGAAACAGGTAGCTT
GGCCAGAACTAATAGCAATTCGAAAGGCATGTATTAGCTTGGAAGAAGAT
TACCGGCCAGGAATAACTTATATTGTGGTGCAAAAAAGACATCACACACG
ACTCTTCTGTGCAGATAAAACAGAAAGGGTAGGGAAAAGTGGCAATGTAC
CAGCAGGCACTACAGTGGATAGTACCATCACACATCCATCTGAGTTTGAC
TTTTACCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTCCCTCACA
TTACCAGGTCTTGTGGGATGACAACTGCTTCACTGCAGATGAACTCCAGC
TACTGACTTACCAGCTGTGTCACACCTATGTGAGGTGCACTCGCTCAGTC
TCTATTCCAGCCCCTGCATATTATGCCCGGCTTGTAGCATTTAGGGCAAG
GTATCATCTGGTGGATAAAGATCATGACAGTGCGGAAGGCAGTCATGTGT
CAGGACAGAGCAACGGCCGGGATCCTCAGGCCTTGGCTAAGGCTGTGCAA
ATCCACCATGATACCCAGCACACGATGTATTTTGCCTGAGCGGCCGCG

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSG
TMEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDV
DIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIG
RDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSV
QALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPA
MWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFT
KEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVA
QYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDN
QTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTE
LTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFA
PQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKH
LKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSP
QTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGK
KPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVR
ELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEED
YRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFD
FYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSV
SIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQ
IHHDTQHTMYFA*AAA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4521
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP-TAP tag.