cDNA Clone PAK2

• Plasmid: pEBG6P-1 PAK2 G258R
• Parent Plasmid: pEBG6P
• Expressed: GST-PAK2 G258R
• Genbank: BC069613
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGTCTGATAACGGAGAACTGGAAGATAAGCCTCCAGCA
CCTCCTGTGCGAATGAGCAGCACCATCTTTAGCACTGGAGGCAAAGACCC
TTTGTCAGCCAATCACAGTTTGAAACCTTTGCCCTCTGTTCCAGAAGAGA
AAAAGCCCAGGCATAAAATCATCTCCATATTCTCAGGCACAGAGAAAGGA
AGTAAAAAGAAGGAAAAGGAACGGCCAGAAATTTCTCCTCCATCTGATTT
TGAGCACACCATCCATGTTGGTTTTGATGCTGTTACTGGAGAATTCACTG
GCATGCCAGAACAGTGGGCTCGATTACTACAGACCTCCAATATCACCAAA
CTAGAGCAAAAGAAGAACCCTCAGGCTGTGCTGGATGTCCTAAAGTTCTA
CGACTCCAACACAGTGAAGCAGAAATATCTGAGCTTTACTCCTCCTGAGA
AAGATGGCTTTCCTTCTGGAACACCAGCACTGAATGCCAAGGGAACAGAA
GCACCCGCAGTAGTGACAGAGGAGGAGGATGATGATGAAGAGACTGCTCC
TCCCGTTATTGCCCCGCGACCGGATCATACGAAATCAATTTACACACGGT
CTGTAATTGACCCTGTTCCTGCACCAGTTGGTGATTCACATGTTGATGGT
GCTGCCAAGTCTTTAGACAAACAGAAAAAGAAGACTAAGATGACAGATGA
AGAGATTATGGAGAAATTAAGAACTATCGTGAGCATAGGTGACCCTAAGA
AAAAATATACAAGATATGAAAAAATTGGACAAAGGGCTTCTGGTACAGTT
TTCACTGCTACTGACGTTGCACTGGGACAGGAGGTTGCTATCAAACAAAT
TAATTTACAGAAACAGCCAAAGAAGGAACTGATCATTAACGAGATTCTGG
TGATGAAAGAATTGAAAAATCCCAACATCGTTAACTTTTTGGACAGTTAC
CTGGTAGGAGATGAATTGTTTGTGGTCATGGAATACCTTGCTGGGGGGTC
ACTCACTGATGTGGTAACAGAAACGTGCATGGATGAAGCACAGATTGCTG
CTGTATGCAGAGAGTGTTTACAGGCATTGGAGTTTTTACATGCTAATCAA
GTGATCCACAGAGACATCAAAAGTGACAATGTACTTTTGGGAATGGAAGG
ATCTGTTAAGCTCACTGACTTTGGTTTCTGTGCCCAGATCACCCCTGAGC
AGAGCAAACGCAGTACCATGGTCGGAACGCCATACTGGATGGCACCAGAG
GTGGTTACACGGAAAGCTTATGGCCCTAAAGTCGACATATGGTCTCTGGG
TATCATGGCTATTGAGATGGTAGAAGGAGAGCCTCCATACCTCAATGAAA
ATCCCTTGAGGGCCTTGTACCTAATAGCAACTAATGGAACCCCAGAACTT
CAGAATCCAGAGAAACTTTCCCCAATATTTCGGGATTTCTTAAATCGATG
TTTGGAAATGGATGTGGAAAAAAGGGGTTCAGCCAAAGAATTATTACAGC
ATCCTTTCCTGAAACTGGCCAAACCGTTATCTAGCTTGACACCACTGATC
ATGGCAGCTAAAGAAGCAATGAAGAGTAACCGTTAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSDNGELEDKPPA
PPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKG
SKKKEKERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITK
LEQKKNPQAVLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTE
APAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDG
AAKSLDKQKKKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQRASGTV
FTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSY
LVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQ
VIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPE
VVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPEL
QNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLI
MAAKEAMKSNR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4500
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.; Note: This sequence differs from the genomic sequence of PAK2 because there is a G258R mutation in this particular accession.