cDNA Clone EIF2C4

• Plasmid: pCMV5-FLAG-3 EIF2C4
• Parent Plasmid: pCMV5
• Expressed: FLAG-EIF2C4
• Genbank: NM_017629
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGGGATCCCCGGAATTC
CCGGGTCGACATGGAGGCGCTGGGACCCGGACCTCCGGCTAGCCTGTTTC
AGCCACCTCGTCGTCCTGGCCTTGGAACTGTTGGAAAACCAATTCGACTG
TTAGCCAATCATTTTCAGGTTCAGATTCCTAAAATAGATGTGTATCACTA
TGATGTGGATATTAAGCCTGAAAAACGGCCTCGTAGAGTCAACAGGGAGG
TAGTAGATACAATGGTGCGGCACTTCAAGATGCAAATATTTGGTGATCGG
CAGCCTGGGTATGATGGCAAAAGAAACATGTACACAGCACATCCACTACC
AATTGGACGGGATAGGGTTGATATGGAGGTGACTCTTCCAGGCGAGGGTA
AAGACCAAACATTTAAAGTGTCTGTTCAGTGGGTGTCAGTTGTGAGCCTT
CAGTTGCTTTTAGAAGCTTTGGCTGGGCACTTGAATGAAGTCCCAGATGA
CTCAGTACAAGCACTTGATGTTATCACAAGACACCTTCCCTCCATGAGGT
ACACCCCAGTGGGCCGTTCCTTTTTCTCACCCCCGGAAGGTTACTACCAC
CCTCTGGGAGGGGGCAGGGAGGTCTGGTTTGGTTTTCATCAGTCTGTGAG
ACCTGCCATGTGGAATATGATGCTCAACATTGATGTATCTGCAACTGCTT
TCTACCGGGCTCAGCCTATCATTGAGTTCATGTGTGAGGTTTTAGACATT
CAGAACATCAATGAACAGACCAAACCTCTAACAGACTCCCAGCGTGTCAA
ATTTACCAAAGAAATCAGAGGTCTCAAAGTTGAGGTGACCCACTGTGGAC
AGATGAAACGAAAATACCGAGTTTGTAATGTGACTAGACGGCCAGCCAGT
CATCAAACTTTTCCTTTGCAGCTAGAAAACGGTCAAGCTATGGAATGTAC
AGTAGCTCAATATTTTAAGCAAAAGTATAGTCTGCAACTGAAATACCCCC
ATCTTCCCTGTCTCCAAGTGGGACAAGAACAAAAGCATACATACTTGCCA
CTCGAGGTCTGTAATATAGTGGCAGGACAGCGATGTATCAAGAAGCTCAC
AGACAATCAGACTTCCACAATGATCAAAGCTACAGCAAGATCTGCTCCTG
ACAGACAGGAAGAGATCAGTAGACTGGTGAAGAGCAACAGTATGGTGGGT
GGACCTGATCCATACCTTAAAGAATTTGGTATTGTTGTCCACAATGAAAT
GACAGAGCTCACAGGCAGGGTACTTCCAGCACCAATGCTGCAATATGGAG
GCCGGAATAAAACAGTAGCCACACCCAACCAGGGTGTCTGGGACATGCGA
GGAAAGCAGTTTTATGCTGGCATTGAAATTAAAGTTTGGGCAGTTGCTTG
TTTTGCACCTCAGAAACAATGTAGGGAAGATTTACTAAAGAGTTTCACTG
ACCAGCTGCGTAAAATCTCTAAGGATGCAGGAATGCCCATCCAGGGTCAG
CCATGTTTCTGCAAGTATGCACAAGGTGCAGACAGTGTGGAGCCTATGTT
TAAACATCTGAAAATGACTTATGTGGGCCTACAGCTAATAGTGGTTATCC
TGCCTGGAAAGACACCAGTATATGCGGAGGTGAAACGTGTTGGAGATACC
CTTCTAGGTATGGCCACACAGTGTGTCCAGGTAAAAAATGTAGTGAAGAC
CTCACCTCAAACCCTTTCCAATCTTTGCCTGAAGATAAATGCAAAACTTG
GAGGAATTAACAATGTGCTTGTGCCTCATCAAAGGCCCTCGGTGTTCCAG
CAGCCTGTCATCTTCCTGGGAGCGGATGTCACACACCCCCCAGCAGGGGA
TGGGAAGAAACCTTCCATTGCTGCTGTGGTTGGCAGTATGGATGGCCACC
CCAGCCGGTACTGTGCCACCGTTCGGGTGCAGACTTCCCGGCAGGAGATC
TCCCAAGAGCTCCTCTACAGTCAAGAGGTCATCCAGGACCTGACTAACAT
GGTTCGAGAGCTGCTGATTCAGTTCTACAAATCCACACGCTTCAAACCCA
CTCGGATCATCTATTACCGTGGAGGGGTATCTGAGGGACAAATGAAACAG
GTAGCTTGGCCAGAACTAATAGCAATTCGAAAGGCATGTATTAGCTTGGA
AGAAGATTACCGGCCAGGAATAACTTATATTGTGGTGCAAAAAAGACATC
ACACACGACTCTTCTGTGCAGATAAAACAGAAAGGGTAGGGAAAAGTGGC
AATGTACCAGCAGGCACTACAGTGGATAGTACCATCACACATCCATCTGA
GTTTGACTTTTACCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTC
CCTCACATTACCAGGTCTTGTGGGATGACAACTGCTTCACTGCAGATGAA
CTCCAGCTACTGACTTACCAGCTGTGTCACACCTATGTGAGGTGCACTCG
CTCAGTCTCTATTCCAGCCCCTGCATATTATGCCCGGCTTGTAGCATTTA
GGGCAAGGTATCATCTGGTGGATAAAGATCATGACAGTGCGGAAGGCAGT
CATGTGTCAGGACAGAGCAACGGCCGGGATCCTCAGGCCTTGGCTAAGGC
TGTGCAAATCCACCATGATACCCAGCACACGATGTATTTTGCCTGAGCGG
CCGCG

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMEALGPGPPASLFQPPRRPGLGTVGKPIRLLA
NHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQP
GYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQL
LLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPL
GGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQN
INEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQ
TFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLE
VCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGP
DPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGK
QFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPC
FCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLL
GMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQP
VIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQ
ELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVA
WPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNV
PAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQ
LLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHV
SGQSNGRDPQALAKAVQIHHDTQHTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4495
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal FLAG tag.