cDNA Clone EIF2C4

• Plasmid: pEBG6P-1 EIF2C4
• Parent Plasmid: pEBG6P
• Expressed: GST-EIF2C4
• Genbank: NM_017629
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATGGAGGCGCTGGGACCCGGACCTCCG
GCTAGCCTGTTTCAGCCACCTCGTCGTCCTGGCCTTGGAACTGTTGGAAA
ACCAATTCGACTGTTAGCCAATCATTTTCAGGTTCAGATTCCTAAAATAG
ATGTGTATCACTATGATGTGGATATTAAGCCTGAAAAACGGCCTCGTAGA
GTCAACAGGGAGGTAGTAGATACAATGGTGCGGCACTTCAAGATGCAAAT
ATTTGGTGATCGGCAGCCTGGGTATGATGGCAAAAGAAACATGTACACAG
CACATCCACTACCAATTGGACGGGATAGGGTTGATATGGAGGTGACTCTT
CCAGGCGAGGGTAAAGACCAAACATTTAAAGTGTCTGTTCAGTGGGTGTC
AGTTGTGAGCCTTCAGTTGCTTTTAGAAGCTTTGGCTGGGCACTTGAATG
AAGTCCCAGATGACTCAGTACAAGCACTTGATGTTATCACAAGACACCTT
CCCTCCATGAGGTACACCCCAGTGGGCCGTTCCTTTTTCTCACCCCCGGA
AGGTTACTACCACCCTCTGGGAGGGGGCAGGGAGGTCTGGTTTGGTTTTC
ATCAGTCTGTGAGACCTGCCATGTGGAATATGATGCTCAACATTGATGTA
TCTGCAACTGCTTTCTACCGGGCTCAGCCTATCATTGAGTTCATGTGTGA
GGTTTTAGACATTCAGAACATCAATGAACAGACCAAACCTCTAACAGACT
CCCAGCGTGTCAAATTTACCAAAGAAATCAGAGGTCTCAAAGTTGAGGTG
ACCCACTGTGGACAGATGAAACGAAAATACCGAGTTTGTAATGTGACTAG
ACGGCCAGCCAGTCATCAAACTTTTCCTTTGCAGCTAGAAAACGGTCAAG
CTATGGAATGTACAGTAGCTCAATATTTTAAGCAAAAGTATAGTCTGCAA
CTGAAATACCCCCATCTTCCCTGTCTCCAAGTGGGACAAGAACAAAAGCA
TACATACTTGCCACTCGAGGTCTGTAATATAGTGGCAGGACAGCGATGTA
TCAAGAAGCTCACAGACAATCAGACTTCCACAATGATCAAAGCTACAGCA
AGATCTGCTCCTGACAGACAGGAAGAGATCAGTAGACTGGTGAAGAGCAA
CAGTATGGTGGGTGGACCTGATCCATACCTTAAAGAATTTGGTATTGTTG
TCCACAATGAAATGACAGAGCTCACAGGCAGGGTACTTCCAGCACCAATG
CTGCAATATGGAGGCCGGAATAAAACAGTAGCCACACCCAACCAGGGTGT
CTGGGACATGCGAGGAAAGCAGTTTTATGCTGGCATTGAAATTAAAGTTT
GGGCAGTTGCTTGTTTTGCACCTCAGAAACAATGTAGGGAAGATTTACTA
AAGAGTTTCACTGACCAGCTGCGTAAAATCTCTAAGGATGCAGGAATGCC
CATCCAGGGTCAGCCATGTTTCTGCAAGTATGCACAAGGTGCAGACAGTG
TGGAGCCTATGTTTAAACATCTGAAAATGACTTATGTGGGCCTACAGCTA
ATAGTGGTTATCCTGCCTGGAAAGACACCAGTATATGCGGAGGTGAAACG
TGTTGGAGATACCCTTCTAGGTATGGCCACACAGTGTGTCCAGGTAAAAA
ATGTAGTGAAGACCTCACCTCAAACCCTTTCCAATCTTTGCCTGAAGATA
AATGCAAAACTTGGAGGAATTAACAATGTGCTTGTGCCTCATCAAAGGCC
CTCGGTGTTCCAGCAGCCTGTCATCTTCCTGGGAGCGGATGTCACACACC
CCCCAGCAGGGGATGGGAAGAAACCTTCCATTGCTGCTGTGGTTGGCAGT
ATGGATGGCCACCCCAGCCGGTACTGTGCCACCGTTCGGGTGCAGACTTC
CCGGCAGGAGATCTCCCAAGAGCTCCTCTACAGTCAAGAGGTCATCCAGG
ACCTGACTAACATGGTTCGAGAGCTGCTGATTCAGTTCTACAAATCCACA
CGCTTCAAACCCACTCGGATCATCTATTACCGTGGAGGGGTATCTGAGGG
ACAAATGAAACAGGTAGCTTGGCCAGAACTAATAGCAATTCGAAAGGCAT
GTATTAGCTTGGAAGAAGATTACCGGCCAGGAATAACTTATATTGTGGTG
CAAAAAAGACATCACACACGACTCTTCTGTGCAGATAAAACAGAAAGGGT
AGGGAAAAGTGGCAATGTACCAGCAGGCACTACAGTGGATAGTACCATCA
CACATCCATCTGAGTTTGACTTTTACCTCTGTAGTCATGCAGGAATTCAG
GGAACCAGCCGTCCCTCACATTACCAGGTCTTGTGGGATGACAACTGCTT
CACTGCAGATGAACTCCAGCTACTGACTTACCAGCTGTGTCACACCTATG
TGAGGTGCACTCGCTCAGTCTCTATTCCAGCCCCTGCATATTATGCCCGG
CTTGTAGCATTTAGGGCAAGGTATCATCTGGTGGATAAAGATCATGACAG
TGCGGAAGGCAGTCATGTGTCAGGACAGAGCAACGGCCGGGATCCTCAGG
CCTTGGCTAAGGCTGTGCAAATCCACCATGATACCCAGCACACGATGTAT
TTTGCCTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMEALGPGPP
ASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRR
VNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTL
PGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHL
PSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDV
SATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEV
THCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQ
LKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATA
RSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPM
LQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLL
KSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQL
IVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKI
NAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGS
MDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKST
RFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVV
QKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQ
GTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAR
LVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMY
FA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4466
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.