cDNA Clone EIF2C1

• Plasmid: pEGFP-C2-TAP EIF2C1
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP EIF2C1
• Genbank: NM_012199
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGAAGCG
GGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAGGT
GTTCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATCA
AGCTCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGTAC
CACTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACCG
GGAAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGGTG
ATCGCAAGCCTGTGTATGATGGAAAGAAGAACATTTACACTGTCACAGCA
CTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCCCTGGGGA
AGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGA
GCTGGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCCC
TTGGAGTCTGTGCAAGCCCTGGATGTGGCCATGAGGCACCTGGCATCCAT
GAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACT
ACCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCT
GTGCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCTCAGCCAC
TGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCTGG
ACATCAGGAACATAGATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCGC
GTTCGCTTCACCAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACTG
TGGACAGATGAAGAGGAAGTACCGCGTGTGTAATGTTACCCGTCGCCCTG
CTAGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGGAG
TGCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTA
TCCCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTACC
TTCCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAAAG
CTGACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCCGC
TCCAGACAGACAGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTACA
ACTTAGATCCCTACATCCAGGAATTTGGGATCAAAGTGAAGGATGACATG
ACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGG
CCGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGGG
GGAAACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCTGC
TTCGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACAGA
CCAGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCAAC
CTTGTTTCTGCAAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGTTC
CGGCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTGTCATCCT
GCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACAC
TCTTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGACC
TCACCTCAGACTCTGTCCAACCTCTGCCTCAAGATCAATGTCAAACTTGG
TGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCGTTTTTCAAC
AGCCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGGAT
GGGAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCACCC
CAGCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGATCA
TTGAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAAG
TCCACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTGCC
TGAAGGCCAGCTACCCCAGATACTCCACTATGAGCTACTGGCCATTCGTG
ATGCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATCACTTATATT
GTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAATGA
GCGAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACCA
ACATCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAGGC
ATCCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGACAA
CCGTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCACA
CTTACGTACGATGCACACGCTCTGTCTCTATCCCAGCACCTGCCTACTAT
GCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAGCA
TGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGACC
CCCAGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCACC
ATGTACTTCGCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMEA
GPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVY
HYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTA
LPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVP
LESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQS
VRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQR
VRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVE
CTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKK
LTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDM
TEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIAC
FAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMF
RHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKT
SPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGD
GKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYK
STRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYI
VVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAG
IQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYY
ARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRT
MYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4451
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP-TAP tag