cDNA Clone EIF2C2

• Plasmid: pEGFP-C2-TAP EIF2C2
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP EIF2C2
• Genbank: NM_012154
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTACTCG
GGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAAGG
ATATGCCTTCAAGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAGAA
CAATCAAATTACAGGCCAATTTCTTCGAAATGGACATCCCCAAAATTGAC
ATCTATCATTATGAATTGGATATCAAGCCAGAGAAGTGCCCGAGGAGAGT
TAACAGGGAAATCGTGGAACACATGGTCCAGCACTTTAAAACACAGATCT
TTGGGGATCGGAAGCCCGTGTTTGACGGCAGGAAGAATCTATACACAGCC
ATGCCCCTTCCGATTGGGAGGGACAAGGTGGAGCTGGAGGTCACGCTGCC
AGGAGAAGGCAAGGATCGCATCTTCAAGGTGTCCATCAAGTGGGTGTCCT
GCGTGAGCTTGCAGGCGTTACACGATGCACTTTCAGGGCGGCTGCCCAGC
GTCCCTTTTGAGACGATCCAGGCCCTGGACGTGGTCATGAGGCACTTGCC
ATCCATGAGGTACACCCCCGTGGGCCGCTCCTTCTTCACCGCGTCCGAAG
GCTGCTCTAACCCTCTTGGCGGGGGCCGAGAAGTGTGGTTTGGCTTCCAT
CAGTCCGTCCGGCCTTCTCTCTGGAAAATGATGCTGAATATTGATGTGTC
AGCAACAGCGTTTTACAAGGCACAGCCAGTAATCGAGTTTGTTTGTGAAG
TTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATTCC
CAAAGGGTAAAGTTTACCAAAGAAATTAAAGGTCTAAAGGTGGAGATAAC
GCACTGTGGGCAGATGAAGAGGAAGTACCGCGTCTGCAATGTGACCCGGC
GGCCCGCCAGTCACCAAACATTCCCGCTGCAGCAGGAGAGCGGGCAGACG
GTGGAGTGCACGGTGGCCCAGTATTTCAAGGACAGGCACAAGTTGGTTCT
GCGCTACCCCCACCTCCCATGTTTACAAGTCGGACAGGAGCAGAAACACA
CCTACCTTCCCCTGGAGGTCTGTAACATTGTGGCAGGACAAAGATGTATT
AAAAAATTAACGGACAATCAGACCTCAACCATGATCAGAGCGACTGCTAG
GTCGGCGCCCGATCGGCAAGAAGAGATTAGCAAATTGATGCGAAGTGCAA
GTTTCAACACAGATCCATACGTCCGTGAATTTGGAATCATGGTCAAAGAT
GAGATGACAGACGTGACTGGGCGGGTGCTGCAGCCGCCCTCCATCCTCTA
CGGGGGCAGGAATAAAGCTATTGCGACCCCTGTCCAGGGCGTCTGGGACA
TGCGGAACAAGCAGTTCCACACGGGCATCGAGATCAAGGTGTGGGCCATT
GCGTGCTTCGCCCCCCAGCGCCAGTGCACGGAAGTCCATCTGAAGTCCTT
CACAGAGCAGCTCAGAAAGATCTCGAGAGACGCCGGCATGCCCATCCAGG
GCCAGCCGTGCTTCTGCAAATACGCGCAGGGGGCGGACAGCGTGGAGCCC
ATGTTCCGGCACCTGAAGAACACGTATGCGGGCCTGCAGCTGGTGGTGGT
CATCCTGCCCGGCAAGACGCCCGTGTACGCCGAGGTCAAGCGCGTGGGAG
ACACGGTGCTGGGGATGGCCACGCAGTGCGTGCAGATGAAGAACGTGCAG
AGGACCACGCCACAGACCCTGTCCAACCTCTGCCTGAAGATCAACGTCAA
GCTGGGAGGCGTGAACAACATCCTGCTGCCCCAGGGCAGGCCGCCGGTGT
TCCAGCAGCCCGTCATCTTTCTGGGAGCAGACGTCACTCACCCCCCCGCC
GGGGATGGGAAGAAGCCCTCCATTGCCGCCGTGGTGGGCAGCATGGACGC
CCACCCCAATCGCTACTGCGCCACCGTGCGTGTGCAGCAGCACCGGCAGG
AGATCATACAAGACCTGGCCGCCATGGTCCGCGAGCTCCTCATCCAGTTC
TACAAGTCCACGCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGACGG
TGTCTCTGAAGGCCAGTTCCAGCAGGTTCTCCACCACGAGTTGCTGGCCA
TCCGTGAGGCCTGTATCAAGCTAGAAAAAGACTACCAGCCCGGGATCACC
TTCATCGTGGTGCAGAAGAGGCACCACACCCGGCTCTTCTGCACTGACAA
GAACGAGCGGGTTGGGAAAAGTGGAAACATTCCAGCAGGCACGACTGTGG
ACACGAAAATCACCCACCCCACCGAGTTCGACTTCTACCTGTGTAGTCAC
GCTGGCATCCAGGGGACAAGCAGGCCTTCGCACTATCACGTCCTCTGGGA
CGACAATCGTTTCTCCTCTGATGAGCTGCAGATCCTAACCTACCAGCTGT
GTCACACCTACGTGCGCTGCACACGCTCCGTGTCCATCCCAGCGCCAGCA
TACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCACCTGGTGGATAA
GGAACATGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGGGC
GAGACCACCAAGCACTGGCCAAGGCGGTCCAGGTTCACCAAGACACTCTG
CGCACCATGTACTTTGCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMYS
GAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKID
IYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTA
MPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPS
VPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFH
QSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDS
QRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQT
VECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCI
KKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKD
EMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAI
ACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEP
MFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQ
RTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPA
GDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQF
YKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGIT
FIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSH
AGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPA
YYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTL
RTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP-TAP taq