cDNA Clone EIF2C1

• Plasmid: pCMV5-FLAG-1 EIF2C1
• Parent Plasmid: pCMV5
• Expressed: FLAG-EIF2C1
• Genbank: NM_012199
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGGAAGCGGG
ACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAGGTGT
TCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATCAAG
CTCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGTACCA
CTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACCGGG
AAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGGTGAT
CGCAAGCCTGTGTATGATGGAAAGAAGAACATTTACACTGTCACAGCACT
GCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCCCTGGGGAAG
GGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGC
TGGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCCCTT
GGAGTCTGTGCAAGCCCTGGATGTGGCCATGAGGCACCTGGCATCCATGA
GGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTAC
CACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGT
GCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCTCAGCCACTG
CCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCTGGAC
ATCAGGAACATAGATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCGCGT
TCGCTTCACCAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACTGTG
GACAGATGAAGAGGAAGTACCGCGTGTGTAATGTTACCCGTCGCCCTGCT
AGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGGAGTG
CACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATC
CCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTACCTT
CCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAAAGCT
GACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCCGCTC
CAGACAGACAGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTACAAC
TTAGATCCCTACATCCAGGAATTTGGGATCAAAGTGAAGGATGACATGAC
GGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGCC
GGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGGGGG
AAACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCTGCTT
CGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACAGACC
AGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCAACCT
TGTTTCTGCAAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGTTCCG
GCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTGTCATCCTGC
CAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACACTC
TTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGACCTC
ACCTCAGACTCTGTCCAACCTCTGCCTCAAGATCAATGTCAAACTTGGTG
GCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCGTTTTTCAACAG
CCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGGATGG
GAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCACCCCA
GCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGATCATT
GAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAAGTC
CACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTGCCTG
AAGGCCAGCTACCCCAGATACTCCACTATGAGCTACTGGCCATTCGTGAT
GCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATCACTTATATTGT
GGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAATGAGC
GAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACCAAC
ATCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAGGCAT
CCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGACAACC
GTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCACACT
TACGTACGATGCACACGCTCTGTCTCTATCCCAGCACCTGCCTACTATGC
CCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAGCATG
ACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGACCCC
CAGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCACCAT
GTACTTCGCTTGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLL
ANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRK
PVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWR
MLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHP
LGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIR
NIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASH
QTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPL
EVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLD
PYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQ
FYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCF
CKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLG
MATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPV
IFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIED
LSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDAC
IKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNIT
HPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYV
RCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQA
LAKAVQVHQDTLRTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4445
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal FLAG tag.