cDNA Clone EIF2C1

• Plasmid: pGEX6P-1-EIF2C1
• Parent Plasmid: pGEX6P1
• Expressed: GST-EIF2C1
• Genbank: NM_012199
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGAAG
CGGGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAG
GTGTTCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAAT
CAAGCTCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGT
ACCACTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAAC
CGGGAAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGG
TGATCGCAAGCCTGTGTATGATGGAAAGAAGAACATTTACACTGTCACAG
CACTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCCCTGGG
GAAGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGT
GAGCTGGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTC
CCTTGGAGTCTGTGCAAGCCCTGGATGTGGCCATGAGGCACCTGGCATCC
ATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTA
CTACCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGT
CTGTGCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCTCAGCC
ACTGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCT
GGACATCAGGAACATAGATGAGCAGCCCAAGCCCCTCACGGACTCTCAGC
GCGTTCGCTTCACCAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCAC
TGTGGACAGATGAAGAGGAAGTACCGCGTGTGTAATGTTACCCGTCGCCC
TGCTAGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGG
AGTGCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAG
TATCCCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTA
CCTTCCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAA
AGCTGACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCC
GCTCCAGACAGACAGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTA
CAACTTAGATCCCTACATCCAGGAATTTGGGATCAAAGTGAAGGATGACA
TGACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGC
GGCCGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCG
GGGGAAACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCT
GCTTCGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACA
GACCAGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCA
ACCTTGTTTCTGCAAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGT
TCCGGCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTGTCATC
CTGCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATAC
ACTCTTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGA
CCTCACCTCAGACTCTGTCCAACCTCTGCCTCAAGATCAATGTCAAACTT
GGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCGTTTTTCA
ACAGCCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGG
ATGGGAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCAC
CCCAGCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGAT
CATTGAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACA
AGTCCACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTG
CCTGAAGGCCAGCTACCCCAGATACTCCACTATGAGCTACTGGCCATTCG
TGATGCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATCACTTATA
TTGTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAAT
GAGCGAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACAC
CAACATCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAG
GCATCCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGAC
AACCGTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCA
CACTTACGTACGATGCACACGCTCTGTCTCTATCCCAGCACCTGCCTACT
ATGCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAG
CATGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGA
CCCCCAGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCA
CCATGTACTTCGCTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEAGPSGAAAGAYLPPLQQ
VFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVN
REVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPG
EGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLAS
MRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSA
TAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTH
CGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLK
YPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARS
APDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYG
GRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFT
DQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVI
LPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKL
GGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAH
PSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGV
PEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKN
ERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDD
NRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKE
HDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4444
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.