cDNA Clone EIF2C1

• Plasmid: pEBG6P-1 EIF2C1
• Parent Plasmid: pEBG6P
• Expressed: GST-EIF2C1
• Genbank: NM_012199
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGGAAGCGGGACCCTCGGGAGCAGCTGCGGGCGCTTAC
CTGCCCCCCCTGCAGCAGGTGTTCCAGGCACCTCGCCGGCCTGGCATTGG
CACTGTGGGGAAACCAATCAAGCTCCTGGCCAATTACTTTGAGGTGGACA
TCCCTAAGATCGACGTGTACCACTACGAGGTGGACATCAAGCCGGATAAG
TGTCCCCGTAGAGTCAACCGGGAAGTGGTGGAATACATGGTCCAGCATTT
CAAGCCTCAGATCTTTGGTGATCGCAAGCCTGTGTATGATGGAAAGAAGA
ACATTTACACTGTCACAGCACTGCCCATTGGCAACGAACGGGTCGACTTT
GAGGTGACAATCCCTGGGGAAGGGAAGGATCGAATCTTTAAGGTCTCCAT
CAAGTGGCTAGCCATTGTGAGCTGGCGAATGCTGCATGAGGCCCTGGTCA
GCGGCCAGATCCCTGTTCCCTTGGAGTCTGTGCAAGCCCTGGATGTGGCC
ATGAGGCACCTGGCATCCATGAGGTACACCCCTGTGGGCCGCTCCTTCTT
CTCACCGCCTGAGGGCTACTACCACCCGCTGGGGGGTGGGCGCGAGGTCT
GGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAGATGATGCTC
AACATTGATGTCTCAGCCACTGCCTTTTATAAGGCACAGCCAGTGATTGA
GTTCATGTGTGAGGTGCTGGACATCACGAACATAGATGAGCAGCCCAAGC
CCCTCACGGACTCTCAGCGCGTTCGCTTCACCAAGGAGATCAAGGGCCTG
AAGGTGGAAGTCACCCACTGTGGACAGATGAAGAGGAAGTACCGCGTGTG
TAATGTTACCCGTCGCCCTGCTAGCCATCAGACATTCCCCTTACAGCTGG
AGAGTGGACAGACTGTGGAGTGCACAGTGGCACAGTATTTCAAGCAGAAA
TATAACCTTCAGCTCAAGTATCCCCATCTGCCCTGCCTACAAGTTGGCCA
GGAACAAAAGCATACCTACCTTCCCCTAGAGGTCTGTAACATTGTGGCTG
GGCAGCGCTGTATTAAAAAGCTGACCGACAACCAGACCTCGACCATGATA
AAGGCCACAGCTAGATCCGCTCCAGACAGACAGGAGGAGATCAGTCGCCT
GATGAAGAATGCCAGCTACAACTTAGATCCCTACATCCAGGAATTTGGGA
TCAAAGTGAAGGATGACATGACGGAGGTGACAGGGCGAGTGCTGCCGGCG
CCCATCTTGCAGTACGGCGGCCGGAACCGGGCCATTGCCACACCCAATCA
GGGTGTCTGGGACATGCGGGGGAAACAGTTCTACAATGGGATTGAGATCA
AAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAGTGTCGAGAAGAG
GTGCTCAAGAACTTCACAGACCAGCTGCGGAAGATTTCCAAGGATGCGGG
GATGCCTATCCAGGGTCAACCTTGTTTCTGCAAATATGCACAGGGGGCAG
ACAGCGTGGAGCCTATGTTCCGGCATCTCAAGAACACCTACTCAGGGCTG
CAGCTCATTATTGTCATCCTGCCAGGGAAGACGCCGGTGTATGCTGAGGT
GAAACGTGTCGGAGATACACTCTTGGGAATGGCTACGCAGTGTGTGCAGG
TGAAGAACGTGGTCAAGACCTCACCTCAGACTCTGTCCAACCTCTGCCTC
AAGATCAATGTCAAACTTGGTGGCATTAACAACATCCTAGTCCCACACCA
GCGCTCTGCCGTTTTTCAACAGCCAGTGATATTCCTGGGAGCAGATGTTA
CACACCCCCCAGCAGGGGATGGGAAAAAACCTTCTATCACAGCAGTGGTA
GGCAGTATGGATGCCCACCCCAGCCGATACTGTGCTACTGTGCGGGTACA
GCGACCACGGCAAGAGATCATTGAAGACTTGTCCTACATGGTGCGTGAGC
TCCTCATCCAATTCTACAAGTCCACCCGTTTCAAGCCTACCCGCATCATC
TTCTACCGAGATGGGGTGCCTGAAGGCCAGCTACCCCAGATACTCCACTA
TGAGCTACTGGCCATTCGTGATGCCTGCATCAAACTGGAAAAGGACTACC
AGCCTGGGATCACTTATATTGTGGTGCAGAAACGCCATCACACCCGCCTT
TTCTGTGCTGACAAGAATGAGCGAATTGGGAAGAGTGGTAACATCCCAGC
TGGGACCACAGTGGACACCAACATCACCCACCCATTTGAGTTTGACTTCT
ATCTGTGCAGCCACGCAGGCATCCAGGGCACCAGCCGACCATCCCATTAC
TATGTTCTTTGGGATGACAACCGTTTCACAGCAGATGAGCTCCAGATCCT
GACGTACCAGCTGTGCCACACTTACGTACGATGCACACGCTCTGTCTCTA
TCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCACGATAC
CACCTGGTGGACAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCGGG
GCAGAGCAATGGGCGGGACCCCCAGGCCCTGGCCAAAGCCGTGCAGGTTC
ACCAGGATACTCTGCGCACCATGTACTTCGCTTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEAGPSGAAAGAY
LPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDK
CPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDF
EVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVA
MRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMML
NIDVSATAFYKAQPVIEFMCEVLDITNIDEQPKPLTDSQRVRFTKEIKGL
KVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQK
YNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMI
KATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPA
PILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREE
VLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGL
QLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCL
KINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVV
GSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRII
FYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRL
FCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHY
YVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARY
HLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4443
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.