cDNA Clone EIF2C1

• Plasmid: pCMV5-HA-1 EIF2C1
• Parent Plasmid: pCMV5
• Expressed: HA-EIF2C1
• Genbank: NM_012199
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGGAAGC
GGGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAGG
TGTTCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATC
AAGCTCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGTA
CCACTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACC
GGGAAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGGT
GATCGCAAGCCTGTGTATGATGGAAAGAAGAACATTTACACTGTCACAGC
ACTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCCCTGGGG
AAGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTG
AGCTGGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCC
CTTGGAGTCTGTGCAAGCCCTGGATGTGGCCATGAGGCACCTGGCATCCA
TGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTAC
TACCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTC
TGTGCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCTCAGCCA
CTGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCTG
GACATCAGGAACATAGATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCG
CGTTCGCTTCACCAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACT
GTGGACAGATGAAGAGGAAGTACCGCGTGTGTAATGTTACCCGTCGCCCT
GCTAGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGGA
GTGCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGT
ATCCCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTAC
CTTCCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAAA
GCTGACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCCG
CTCCAGACAGACAGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTAC
AACTTAGATCCCTACATCCAGGAATTTGGGATCAAAGTGAAGGATGACAT
GACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCG
GCCGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGG
GGGAAACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCTG
CTTCGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACAG
ACCAGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCAA
CCTTGTTTCTGCAAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGTT
CCGGCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTGTCATCC
TGCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACA
CTCTTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGAC
CTCACCTCAGACTCTGTCCAACCTCTGCCTCAAGATCAATGTCAAACTTG
GTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCGTTTTTCAA
CAGCCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGGA
TGGGAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCACC
CCAGCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGATC
ATTGAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAA
GTCCACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTGC
CTGAAGGCCAGCTACCCCAGATACTCCACTATGAGCTACTGGCCATTCGT
GATGCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATCACTTATAT
TGTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAATG
AGCGAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACC
AACATCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAGG
CATCCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGACA
ACCGTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCAC
ACTTACGTACGATGCACACGCTCTGTCTCTATCCCAGCACCTGCCTACTA
TGCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAGC
ATGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGAC
CCCCAGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCAC
CATGTACTTCGCTTGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKL
LANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDR
KPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSW
RMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYH
PLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDI
RNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPAS
HQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLP
LEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNL
DPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGK
QFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPC
FCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLL
GMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQP
VIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIE
DLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDA
CIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNI
THPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTY
VRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQ
ALAKAVQVHQDTLRTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4441
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.