cDNA Clone EPHA2

• Plasmid: pGEX6P-1-EPHA2 591-976
• Parent Plasmid: pGEX6P1
• Expressed: GST-EPHA2 591-976
• Genbank: NM_004431
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTCCCCACA
CATATGAGGACCCCAACCAGGCTGTGTTGAAGTTCACTACCGAGATCCAT
CCATCCTGTGTCACTCGGCAGAAGGTGATCGGAGCAGGAGAGTTTGGGGA
GGTGTACAAGGGCATGCTGAAGACATCCTCGGGGAAGAAGGAGGTGCCGG
TGGCCATCAAGACGCTGAAAGCCGGCTACACAGAGAAGCAGCGAGTGGAC
TTCCTCGGCGAGGCCGGCATCATGGGCCAGTTCAGCCACCACAACATCAT
CCGCCTAGAGGGCGTCATCTCCAAATACAAGCCCATGATGATCATCACTG
AGTACATGGAGAATGGGGCCCTGGACAAGTTCCTTCGGGAGAAGGATGGC
GAGTTCAGCGTGCTGCAGCTGGTGGGCATGCTGCGGGGCATCGCAGCTGG
CATGAAGTACCTGGCCAACATGAACTATGTGCACCGTGACCTGGCTGCCC
GCAACATCCTCGTCAACAGCAACCTGGTCTGCAAGGTGTCTGACTTTGGC
CTGTCCCGCGTGCTGGAGGACGACCCCGAGGCCACCTACACCACCAGTGG
CGGCAAGATCCCCATCCGCTGGACCGCCCCGGAGGCCATTTCCTACCGGA
AGTTCACCTCTGCCAGCGACGTGTGGAGCTTTGGCATTGTCATGTGGGAG
GTGATGACCTATGGCGAGCGGCCCTACTGGGAGTTGTCCAACCACGAGGT
GATGAAAGCCATCAATGATGGCTTCCGGCTCCCCACACCCATGGACTGCC
CCTCCGCCATCTACCAGCTCATGATGCAGTGCTGGCAGCAGGAGCGTGCC
CGCCGCCCCAAGTTCGCTGACATCGTCAGCATCCTGGACAAGCTCATTCG
TGCCCCTGACTCCCTCAAGACCCTGGCTGACTTTGACCCCCGCGTGTCTA
TCCGGCTCCCCAGCACGAGCGGCTCGGAGGGGGTGCCCTTCCGCACGGTG
TCCGAGTGGCTGGAGTCCATCAAGATGCAGCAGTATACGGAGCACTTCAT
GGCGGCCGGCTACACTGCCATCGAGAAGGTGGTGCAGATGACCAACGACG
ACATCAAGAGGATTGGGGTGCGGCTGCCCGGCCACCAGAAGCGCATCGCC
TACAGCCTGCTGGGACTCAAGGACCAGGTGAACACTGTGGGGATCCCCAT
CTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPHTYEDPNQAVLKFTTEIH
PSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVD
FLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDG
EFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFG
LSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWE
VMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERA
RRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTV
SEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIA
YSLLGLKDQVNTVGIPI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4438
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.