cDNA Clone EIF2C2

• Plasmid: pEBG6P-1 EIF2C2
• Parent Plasmid: pEBG6P
• Expressed: GST-EIF2C2
• Genbank: NM_012154
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGTACTCGGGAGCCGGCCCCGCACTTGCACCTCCTGCG
CCGCCGCCCCCCATCCAAGGATATGCCTTCAAGCCTCCACCTAGACCCGA
CTTTGGGACCTCCGGGAGAACAATCAAATTACAGGCCAATTTCTTCGAAA
TGGACATCCCCAAAATTGACATCTATCATTATGAATTGGATATCAAGCCA
GAGAAGTGCCCGAGGAGAGTTAACAGGGAAATCGTGGAACACATGGTCCA
GCACTTTAAAACACAGATCTTTGGGGATCGGAAGCCCGTGTTTGACGGCA
GGAAGAATCTATACACAGCCATGCCCCTTCCGATTGGGAGGGACAAGGTG
GAGCTGGAGGTCACGCTGCCAGGAGAAGGCAAGGATCGCATCTTCAAGGT
GTCCATCAAGTGGGTGTCCTGCGTGAGCTTGCAGGCGTTACACGATGCAC
TTTCAGGGCGGCTGCCCAGCGTCCCTTTTGAGACGATCCAGGCCCTGGAC
GTGGTCATGAGGCACTTGCCATCCATGAGGTACACCCCCGTGGGCCGCTC
CTTCTTCACCGCGTCCGAAGGCTGCTCTAACCCTCTTGGCGGGGGCCGAG
AAGTGTGGTTTGGCTTCCATCAGTCCGTCCGGCCTTCTCTCTGGAAAATG
ATGCTGAATATTGATGTGTCAGCAACAGCGTTTTACAAGGCACAGCCAGT
AATCGAGTTTGTTTGTGAAGTTTTGGATTTTAAAAGTATTGAAGAACAAC
AAAAACCTCTGACAGATTCCCAAAGGGTAAAGTTTACCAAAGAAATTAAA
GGTCTAAAGGTGGAGATAACGCACTGTGGGCAGATGAAGAGGAAGTACCG
CGTCTGCAATGTGACCCGGCGGCCCGCCAGTCACCAAACATTCCCGCTGC
AGCAGGAGAGCGGGCAGACGGTGGAGTGCACGGTGGCCCAGTATTTCAAG
GACAGGCACAAGTTGGTTCTGCGCTACCCCCACCTCCCATGTTTACAAGT
CGGACAGGAGCAGAAACACACCTACCTTCCCCTGGAGGTCTGTAACATTG
TGGCAGGACAAAGATGTATTAAAAAATTAACGGACAATCAGACCTCAACC
ATGATCAGAGCGACTGCTAGGTCGGCGCCCGATCGGCAAGAAGAGATTAG
CAAATTGATGCGAAGTGCAAGTTTCAACACAGATCCATACGTCCGTGAAT
TTGGAATCATGGTCAAAGATGAGATGACAGACGTGACTGGGCGGGTGCTG
CAGCCGCCCTCCATCCTCTACGGGGGCAGGAATAAAGCTATTGCGACCCC
TGTCCAGGGCGTCTGGGACATGCGGAACAAGCAGTTCCACACGGGCATCG
AGATCAAGGTGTGGGCCATTGCGTGCTTCGCCCCCCAGCGCCAGTGCACG
GAAGTCCATCTGAAGTCCTTCACAGAGCAGCTCAGAAAGATCTCGAGAGA
CGCCGGCATGCCCATCCAGGGCCAGCCGTGCTTCTGCAAATACGCGCAGG
GGGCGGACAGCGTGGAGCCCATGTTCCGGCACCTGAAGAACACGTATGCG
GGCCTGCAGCTGGTGGTGGTCATCCTGCCCGGCAAGACGCCCGTGTACGC
CGAGGTCAAGCGCGTGGGAGACACGGTGCTGGGGATGGCCACGCAGTGCG
TGCAGATGAAGAACGTGCAGAGGACCACGCCACAGACCCTGTCCAACCTC
TGCCTGAAGATCAACGTCAAGCTGGGAGGCGTGAACAACATCCTGCTGCC
CCAGGGCAGGCCGCCGGTGTTCCAGCAGCCCGTCATCTTTCTGGGAGCAG
ACGTCACTCACCCCCCCGCCGGGGATGGGAAGAAGCCCTCCATTGCCGCC
GTGGTGGGCAGCATGGACGCCCACCCCAATCGCTACTGCGCCACCGTGCG
TGTGCAGCAGCACCGGCAGGAGATCATACAAGACCTGGCCGCCATGGTCC
GCGAGCTCCTCATCCAGTTCTACAAGTCCACGCGCTTCAAGCCCACCCGC
ATCATCTTCTACCGCGACGGTGTCTCTGAAGGCCAGTTCCAGCAGGTTCT
CCACCACGAGTTGCTGGCCATCCGTGAGGCCTGTATCAAGCTAGAAAAAG
ACTACCAGCCCGGGATCACCTTCATCGTGGTGCAGAAGAGGCACCACACC
CGGCTCTTCTGCACTGACAAGAACGAGCGGGTTGGGAAAAGTGGAAACAT
TCCAGCAGGCACGACTGTGGACACGAAAATCACCCACCCCACCGAGTTCG
ACTTCTACCTGTGTAGTCACGCTGGCATCCAGGGGACAAGCAGGCCTTCG
CACTATCACGTCCTCTGGGACGACAATCGTTTCTCCTCTGATGAGCTGCA
GATCCTAACCTACCAGCTGTGTCACACCTACGTGCGCTGCACACGCTCCG
TGTCCATCCCAGCGCCAGCATACTACGCTCACCTGGTGGCCTTCCGGGCC
AGGTACCACCTGGTGGATAAGGAACATGACAGTGCTGAAGGAAGCCATAC
CTCTGGGCAGAGTAACGGGCGAGACCACCAAGCACTGGCCAAGGCGGTCC
AGGTTCACCAAGACACTCTGCGCACCATGTACTTTGCTTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMYSGAGPALAPPA
PPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKP
EKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKV
ELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALD
VVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKM
MLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIK
GLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFK
DRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTST
MIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVL
QPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCT
EVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYA
GLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNL
CLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAA
VVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTR
IIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHT
RLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPS
HYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRA
RYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.