cDNA Clone EIF2C2

• Plasmid: pGEX6P-1-EIF2C2
• Parent Plasmid: pGEX6P1
• Expressed: GST-EIF2C2
• Genbank: NM_012154
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTACT
CGGGAGCCGGCCCCGCACTTGCACCTCCTGCGCCGCCGCCCCCCATCCAA
GGATATGCCTTCAAGCCTCCACCTAGACCCGACTTTGGGACCTCCGGGAG
AACAATCAAATTACAGGCCAATTTCTTCGAAATGGACATCCCCAAAATTG
ACATCTATCATTATGAATTGGATATCAAGCCAGAGAAGTGCCCGAGGAGA
GTTAACAGGGAAATCGTGGAACACATGGTCCAGCACTTTAAAACACAGAT
CTTTGGGGATCGGAAGCCCGTGTTTGACGGCAGGAAGAATCTATACACAG
CCATGCCCCTTCCGATTGGGAGGGACAAGGTGGAGCTGGAGGTCACGCTG
CCAGGAGAAGGCAAGGATCGCATCTTCAAGGTGTCCATCAAGTGGGTGTC
CTGCGTGAGCTTGCAGGCGTTACACGATGCACTTTCAGGGCGGCTGCCCA
GCGTCCCTTTTGAGACGATCCAGGCCCTGGACGTGGTCATGAGGCACTTG
CCATCCATGAGGTACACCCCCGTGGGCCGCTCCTTCTTCACCGCGTCCGA
AGGCTGCTCTAACCCTCTTGGCGGGGGCCGAGAAGTGTGGTTTGGCTTCC
ATCAGTCCGTCCGGCCTTCTCTCTGGAAAATGATGCTGAATATTGATGTG
TCAGCAACAGCGTTTTACAAGGCACAGCCAGTAATCGAGTTTGTTTGTGA
AGTTTTGGATTTTAAAAGTATTGAAGAACAACAAAAACCTCTGACAGATT
CCCAAAGGGTAAAGTTTACCAAAGAAATTAAAGGTCTAAAGGTGGAGATA
ACGCACTGTGGGCAGATGAAGAGGAAGTACCGCGTCTGCAATGTGACCCG
GCGGCCCGCCAGTCACCAAACATTCCCGCTGCAGCAGGAGAGCGGGCAGA
CGGTGGAGTGCACGGTGGCCCAGTATTTCAAGGACAGGCACAAGTTGGTT
CTGCGCTACCCCCACCTCCCATGTTTACAAGTCGGACAGGAGCAGAAACA
CACCTACCTTCCCCTGGAGGTCTGTAACATTGTGGCAGGACAAAGATGTA
TTAAAAAATTAACGGACAATCAGACCTCAACCATGATCAGAGCGACTGCT
AGGTCGGCGCCCGATCGGCAAGAAGAGATTAGCAAATTGATGCGAAGTGC
AAGTTTCAACACAGATCCATACGTCCGTGAATTTGGAATCATGGTCAAAG
ATGAGATGACAGACGTGACTGGGCGGGTGCTGCAGCCGCCCTCCATCCTC
TACGGGGGCAGGAATAAAGCTATTGCGACCCCTGTCCAGGGCGTCTGGGA
CATGCGGAACAAGCAGTTCCACACGGGCATCGAGATCAAGGTGTGGGCCA
TTGCGTGCTTCGCCCCCCAGCGCCAGTGCACGGAAGTCCATCTGAAGTCC
TTCACAGAGCAGCTCAGAAAGATCTCGAGAGACGCCGGCATGCCCATCCA
GGGCCAGCCGTGCTTCTGCAAATACGCGCAGGGGGCGGACAGCGTGGAGC
CCATGTTCCGGCACCTGAAGAACACGTATGCGGGCCTGCAGCTGGTGGTG
GTCATCCTGCCCGGCAAGACGCCCGTGTACGCCGAGGTCAAGCGCGTGGG
AGACACGGTGCTGGGGATGGCCACGCAGTGCGTGCAGATGAAGAACGTGC
AGAGGACCACGCCACAGACCCTGTCCAACCTCTGCCTGAAGATCAACGTC
AAGCTGGGAGGCGTGAACAACATCCTGCTGCCCCAGGGCAGGCCGCCGGT
GTTCCAGCAGCCCGTCATCTTTCTGGGAGCAGACGTCACTCACCCCCCCG
CCGGGGATGGGAAGAAGCCCTCCATTGCCGCCGTGGTGGGCAGCATGGAC
GCCCACCCCAATCGCTACTGCGCCACCGTGCGTGTGCAGCAGCACCGGCA
GGAGATCATACAAGACCTGGCCGCCATGGTCCGCGAGCTCCTCATCCAGT
TCTACAAGTCCACGCGCTTCAAGCCCACCCGCATCATCTTCTACCGCGAC
GGTGTCTCTGAAGGCCAGTTCCAGCAGGTTCTCCACCACGAGTTGCTGGC
CATCCGTGAGGCCTGTATCAAGCTAGAAAAAGACTACCAGCCCGGGATCA
CCTTCATCGTGGTGCAGAAGAGGCACCACACCCGGCTCTTCTGCACTGAC
AAGAACGAGCGGGTTGGGAAAAGTGGAAACATTCCAGCAGGCACGACTGT
GGACACGAAAATCACCCACCCCACCGAGTTCGACTTCTACCTGTGTAGTC
ACGCTGGCATCCAGGGGACAAGCAGGCCTTCGCACTATCACGTCCTCTGG
GACGACAATCGTTTCTCCTCTGATGAGCTGCAGATCCTAACCTACCAGCT
GTGTCACACCTACGTGCGCTGCACACGCTCCGTGTCCATCCCAGCGCCAG
CATACTACGCTCACCTGGTGGCCTTCCGGGCCAGGTACCACCTGGTGGAT
AAGGAACATGACAGTGCTGAAGGAAGCCATACCTCTGGGCAGAGTAACGG
GCGAGACCACCAAGCACTGGCCAAGGCGGTCCAGGTTCACCAAGACACTC
TGCGCACCATGTACTTTGCTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMYSGAGPALAPPAPPPPIQ
GYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRR
VNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTL
PGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHL
PSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDV
SATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEI
THCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLV
LRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATA
RSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSIL
YGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKS
FTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVV
VILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINV
KLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMD
AHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRD
GVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTD
KNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLW
DDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVD
KEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4429
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.