cDNA Clone KIAA0731

• Plasmid: pECFP-C1 KIAA0731
• Parent Plasmid: pECFPC1
• Expressed: CFP-KIAA0731
• Genbank: AB018274 (GI:3882183)
• Tag: CFP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GAATTCGCCGCGGATCGCGTGGTCTGCTTGGCTCGCCGCGCCTCTCCCCC
GTCCGTCCATATTGCTGCAGCCCCCGAGCCGGGAAGCCCGGGCCGCCCCG
GGGGCGGGGGGGAGGGAGGGACGGGACTAGAAGCCTGCCGGGCTCGGGGT
GGGGGCGTCTTGCGAGGAACGGGCGGGGGGGGACGCACGCCTAGGAGGCC
TGGACTGCAGAGTGGGGGGCCTTCCTCCCCCCCCGCCCCGCTAGTGGGCC
TCGGATTTACGGCGGCTGCATCTAACTGGGAGGGGGCCGCACCTCGCGTG
AACCCCGACCCTTCTCTGCAGGGACTGGGGCCCAGCGCCCCGGAGGAAGG
CGTCGCGGGCGCTCTGCTAGCCAAGGTGAACCCGTGGACTAAGAACGCAT
TGCCGCCGGTCCTGACCACCGTGAACGGACAGTCCCCCCCAGAACACTCT
GCTCCAGCCAAGGTGGTGAGGGCAGCTGTTCCTAAACAGCGCAAAGGCAG
CAAGGTTGGTGACTTTGGAGATGCAATCAATTGGCCCACACCTGGAGAGA
TAGCCCACAAGAGTGTTCAGCCACAGTCCCACAAGCCTCAGCCTACCCGT
AAACTGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGAG
TGATAGTAAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAA
AGAATGGAGATGAGGATTGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAAC
AAACACAAGTGGGTTCCATTACAAATAGACATGAAGCCTGAAGTGCCCAG
AGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAGCCTAGACACATAC
CTGCCAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTGCCC
GTGGCCCCCCCCACCCCAGCCTGGCAACCAGAGATCAAACCGGAGCCTGC
CTGGCACGACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGTG
GGGCGCGGGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGG
GGACGCGGCCGGGGTGGCACTCGAACCCATTTTGACTACCAGTTTGGCTA
CCGAAAGTTTGATGGTGTGGAGGGGCCTCGTACGCCCAAGTACATGAACA
ACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGTGTG
GATCAGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACTT
CAGCGTGGACAATTTAGAGCGAGACTTCTTCCTGCGAAGGAAAATGGATG
CTGATGGTTTCCTACCCATCACCCTTATTGCTTCCTTCCACCGAGTGCAG
GCCCTTACCACTGACATTTCACTCATCTTTGCGGCCCTAAAGGACAGCAA
GGTGGTGGAGATCGTTGATGAGAAAGTTCGTAGGAGGGAGGAACCAGAAA
AGTGGCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCCCAG
CTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGAC
AGAGTCGGCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAAA
CAGAGGAGGTCAGCAACCTAAAGACACTACCCAAGGGCCTGTCTGCCAGC
CTGCCTGACCTGGATTCTGAGAACTGGATTGAAGTGAAGAAGAGGCCTCG
GCCATCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCCAGATTTTCCCACC
TGACCTCTCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAGGAT
CAGGATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGAGCA
GATGGATGGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCTG
ACTATGAGATTGATGACAGGGATGTCAACAAGATCCTCATTGTCACCCAG
ACACCACATTACATGCGCCGGCACCCAGGGGGGGACCGCACAGGCAACCA
CACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAAGGTCATTAATGATG
GCCTCTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCTGAG
TATTCCCAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGAT
CAGCCGGGAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATCCCA
ACCAGGAAGTTCCTCCTGGGCCACCTCGGTTCCAGCAAGTTCCTACGGAT
GCCCTGGCCAACAAGTTGTTTGGTGCTCCTGAGCCCTCCACCATCGCCCG
CTCTCTACCAACCACTGTCCCAGAGTCACCAAACTACCGCAACACCAGGA
CCCCTCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACATCA
CGGTTTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCC
TCGAAAAAGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCATG
TGGGCTGGGTGATGGATTCCCGTGAGCACAGGCCCCGTACTGCTTCCATC
AGCTCCAGCCCCTCAGAAGGGACGCCTACAGTTGGCAGCTATGGCTGTAC
CCCTCAGTCATTGCCCAAGTTCCAGCATCCTTCCCATGAACTGCTCAAGG
AAAATGGCTTCACACAACACGTCTACCATAAGTATCGTAGGCGCTGCCTT
AATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACACT
CTTCCGCTTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGT
ATGAGGAGTTCAAGCAGCTGGCTCTGGAGGACGCCAAAGAAGGCTACAGA
TATGGTTTGGAGTGCCTTTTTCGATACTACAGTTATGGCCTGGAAAAGAA
GTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAAACGGTGAAGGACT
ATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTGAAA
TATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACCT
CGGCAAATTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTG
AGGAGGGCAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGGT
GAGGGCAGGAAGCGGTGCCCCTCCCAGTCTTCCAGCAGGCCTGCTGCCAT
GATCAGCCAACCCCCTACACCACCCACCGGCCAGCCTGTCCGGGAAGATG
CCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTGAGTC
GAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFAADRVVCLARRASPPSVHIAAAPEPGSPGRPGGGGEGGTGLEACRARG
GGVLRGTGGGGRTPRRPGLQSGGPSSPPAPLVGLGFTAAASNWEGAAPRV
NPDPSLQGLGPSAPEEGVAGALLAKVNPWTKNALPPVLTTVNGQSPPEHS
APAKVVRAAVPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQSHKPQPTR
KLPPKKDMKEQEKGEGSDSKESPKTKSDESGEEKNGDEDCQRGGQKKKGN
KHKWVPLQIDMKPEVPREKLASRPTRPPEPRHIPANRGEIKGSESATYVP
VAPPTPAWQPEIKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGR
GRGRGGTRTHFDYQFGYRKFDGVEGPRTPKYMNNITYYFDNVSSTELYSV
DQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDADGFLPITLIASFHRVQ
ALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPLPPIVDYSQTDFSQ
LLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSAS
LPDLDSENWIEVKKRPRPSPARPKKSEESRFSHLTSLPQQLPSQQLMSKD
QDEQEELDFLFDEEMEQMDGRKNTFTAWSDEESDYEIDDRDVNKILIVTQ
TPHYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFYYEQDLWAEKFEPE
YSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTD
ALANKLFGAPEPSTIARSLPTTVPESPNYRNTRTPRTPRTPQLKDSSQTS
RFYPVVKEGRTLDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASI
SSSPSEGTPTVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCL
NERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYR
YGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLK
YSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGG
EGRKRCPSQSSSRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4427
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP tag.