cDNA Clone KIAA0731

• Plasmid: pGEX6P-1-KIAA0731
• Parent Plasmid: pGEX6P1
• Expressed: GST-KIAA0731
• Genbank: AB018274 (GI:3882183)
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGCCGCGGATCGCGTGGTCTGCTTGGCTCGCCGCGCCTCTCCCCCGTCC
GTCCATATTGCTGCAGCCCCCGAGCCGGGAAGCCCGGGCCGCCCCGGGGG
CGGGGGGGAGGGAGGGACGGGACTAGAAGCCTGCCGGGCTCGGGGTGGGG
GCGTCTTGCGAGGAACGGGCGGGGGGGGACGCACGCCTAGGAGGCCTGGA
CTGCAGAGTGGGGGGCCTTCCTCCCCCCCCGCCCCGCTAGTGGGCCTCGG
ATTTACGGCGGCTGCATCTAACTGGGAGGGGGCCGCACCTCGCGTGAACC
CCGACCCTTCTCTGCAGGGACTGGGGCCCAGCGCCCCGGAGGAAGGCGTC
GCGGGCGCTCTGCTAGCCAAGGTGAACCCGTGGACTAAGAACGCATTGCC
GCCGGTCCTGACCACCGTGAACGGACAGTCCCCCCCAGAACACTCTGCTC
CAGCCAAGGTGGTGAGGGCAGCTGTTCCTAAACAGCGCAAAGGCAGCAAG
GTTGGTGACTTTGGAGATGCAATCAATTGGCCCACACCTGGAGAGATAGC
CCACAAGAGTGTTCAGCCACAGTCCCACAAGCCTCAGCCTACCCGTAAAC
TGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGAGTGAT
AGTAAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAAAGAA
TGGAGATGAGGATTGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAACAAAC
ACAAGTGGGTTCCATTACAAATAGACATGAAGCCTGAAGTGCCCAGAGAG
AAACTGGCTTCACGCCCCACTCGCCCACCGGAGCCTAGACACATACCTGC
CAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTGCCCGTGG
CCCCCCCCACCCCAGCCTGGCAACCAGAGATCAAACCGGAGCCTGCCTGG
CACGACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGTGGGGC
GCGGGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGGGGAC
GCGGCCGGGGTGGCACTCGAACCCATTTTGACTACCAGTTTGGCTACCGA
AAGTTTGATGGTGTGGAGGGGCCTCGTACGCCCAAGTACATGAACAACAT
CACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGTGTGGATC
AGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACTTCAGC
GTGGACAATTTAGAGCGAGACTTCTTCCTGCGAAGGAAAATGGATGCTGA
TGGTTTCCTACCCATCACCCTTATTGCTTCCTTCCACCGAGTGCAGGCCC
TTACCACTGACATTTCACTCATCTTTGCGGCCCTAAAGGACAGCAAGGTG
GTGGAGATCGTTGATGAGAAAGTTCGTAGGAGGGAGGAACCAGAAAAGTG
GCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCCCAGCTTC
TCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGACAGAG
TCGGCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAAACAGA
GGAGGTCAGCAACCTAAAGACACTACCCAAGGGCCTGTCTGCCAGCCTGC
CTGACCTGGATTCTGAGAACTGGATTGAAGTGAAGAAGAGGCCTCGGCCA
TCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCCAGATTTTCCCACCTGAC
CTCTCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAGGATCAGG
ATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGAGCAGATG
GATGGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCTGACTA
TGAGATTGATGACAGGGATGTCAACAAGATCCTCATTGTCACCCAGACAC
CACATTACATGCGCCGGCACCCAGGGGGGGACCGCACAGGCAACCACACC
TCGCGTGCCAAGATGAGCGCCGAACTGGCCAAGGTCATTAATGATGGCCT
CTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCTGAGTATT
CCCAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGATCAGC
CGGGAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATCCCAACCA
GGAAGTTCCTCCTGGGCCACCTCGGTTCCAGCAAGTTCCTACGGATGCCC
TGGCCAACAAGTTGTTTGGTGCTCCTGAGCCCTCCACCATCGCCCGCTCT
CTACCAACCACTGTCCCAGAGTCACCAAACTACCGCAACACCAGGACCCC
TCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACATCACGGT
TTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCCTCGA
AAAAGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCATGTGGG
CTGGGTGATGGATTCCCGTGAGCACAGGCCCCGTACTGCTTCCATCAGCT
CCAGCCCCTCAGAAGGGACGCCTACAGTTGGCAGCTATGGCTGTACCCCT
CAGTCATTGCCCAAGTTCCAGCATCCTTCCCATGAACTGCTCAAGGAAAA
TGGCTTCACACAACACGTCTACCATAAGTATCGTAGGCGCTGCCTTAATG
AGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACACTCTTC
CGCTTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGA
GGAGTTCAAGCAGCTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATG
GTTTGGAGTGCCTTTTTCGATACTACAGTTATGGCCTGGAAAAGAAGTTC
CGGCTGGACATATTCAAGGATTTTCAGGAGGAAACGGTGAAGGACTATGA
AGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTGAAATATT
CCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGC
AAATTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTGAGGA
GGGCAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGG
GCAGGAAGCGGTGCCCCTCCCAGTCTTCCAGCAGGCCTGCTGCCATGATC
AGCCAACCCCCTACACCACCCACCGGCCAGCCTGTCCGGGAAGATGCCAA
ATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFAADRVVCLARRASPPS
VHIAAAPEPGSPGRPGGGGEGGTGLEACRARGGGVLRGTGGGGRTPRRPG
LQSGGPSSPPAPLVGLGFTAAASNWEGAAPRVNPDPSLQGLGPSAPEEGV
AGALLAKVNPWTKNALPPVLTTVNGQSPPEHSAPAKVVRAAVPKQRKGSK
VGDFGDAINWPTPGEIAHKSVQPQSHKPQPTRKLPPKKDMKEQEKGEGSD
SKESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDMKPEVPRE
KLASRPTRPPEPRHIPANRGEIKGSESATYVPVAPPTPAWQPEIKPEPAW
HDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTHFDYQFGYR
KFDGVEGPRTPKYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFS
VDNLERDFFLRRKMDADGFLPITLIASFHRVQALTTDISLIFAALKDSKV
VEIVDEKVRRREEPEKWPLPPIVDYSQTDFSQLLNCPEFVPRQHYQKETE
SAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIEVKKRPRP
SPARPKKSEESRFSHLTSLPQQLPSQQLMSKDQDEQEELDFLFDEEMEQM
DGRKNTFTAWSDEESDYEIDDRDVNKILIVTQTPHYMRRHPGGDRTGNHT
SRAKMSAELAKVINDGLFYYEQDLWAEKFEPEYSQIKQEVENFKKVNMIS
REQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPEPSTIARS
LPTTVPESPNYRNTRTPRTPRTPQLKDSSQTSRFYPVVKEGRTLDAKMPR
KRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPTVGSYGCTP
QSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLF
RFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKF
RLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLG
KFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGGEGRKRCPSQSSSRPAAMI
SQPPTPPTGQPVREDAKWTSQHSNTQTLGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4425
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.