cDNA Clone KIAA0731

• Plasmid: pCMV5-HA-1 KIAA0731
• Parent Plasmid: pCMV5
• Expressed: HA-KIAA0731
• Genbank: AB018274 (GI:3882183)
• Tag: HA
• Species: Human

Sequence of Insert

GAATTGGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCCC
GGAATTCGCCGCGGATCGCGTGGTCTGCTTGGCTCGCCGCGCCTCTCCCC
CGTCCGTCCATATTGCTGCAGCCCCCGAGCCGGGAAGCCCGGGCCGCCCC
GGGGGCGGGGGGGAGGGAGGGACGGGACTAGAAGCCTGCCGGGCTCGGGG
TGGGGGCGTCTTGCGAGGAACGGGCGGGGGGGGACGCACGCCTAGGAGGC
CTGGACTGCAGAGTGGGGGGCCTTCCTCCCCCCCCGCCCCGCTAGTGGGC
CTCGGATTTACGGCGGCTGCATCTAACTGGGAGGGGGCCGCACCTCGCGT
GAACCCCGACCCTTCTCTGCAGGGACTGGGGCCCAGCGCCCCGGAGGAAG
GCGTCGCGGGCGCTCTGCTAGCCAAGGTGAACCCGTGGACTAAGAACGCA
TTGCCGCCGGTCCTGACCACCGTGAACGGACAGTCCCCCCCAGAACACTC
TGCTCCAGCCAAGGTGGTGAGGGCAGCTGTTCCTAAACAGCGCAAAGGCA
GCAAGGTTGGTGACTTTGGAGATGCAATCAATTGGCCCACACCTGGAGAG
ATAGCCCACAAGAGTGTTCAGCCACAGTCCCACAAGCCTCAGCCTACCCG
TAAACTGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGA
GTGATAGTAAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAA
AAGAATGGAGATGAGGATTGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAA
CAAACACAAGTGGGTTCCATTACAAATAGACATGAAGCCTGAAGTGCCCA
GAGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAGCCTAGACACATA
CCTGCCAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTGCC
CGTGGCCCCCCCCACCCCAGCCTGGCAACCAGAGATCAAACCGGAGCCTG
CCTGGCACGACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGT
GGGGCGCGGGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGCGGCCG
GGGACGCGGCCGGGGTGGCACTCGAACCCATTTTGACTACCAGTTTGGCT
ACCGAAAGTTTGATGGTGTGGAGGGGCCTCGTACGCCCAAGTACATGAAC
AACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGTGT
GGATCAGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACT
TCAGCGTGGACAATTTAGAGCGAGACTTCTTCCTGCGAAGGAAAATGGAT
GCTGATGGTTTCCTACCCATCACCCTTATTGCTTCCTTCCACCGAGTGCA
GGCCCTTACCACTGACATTTCACTCATCTTTGCGGCCCTAAAGGACAGCA
AGGTGGTGGAGATCGTTGATGAGAAAGTTCGTAGGAGGGAGGAACCAGAA
AAGTGGCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCCCA
GCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGA
CAGAGTCGGCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAA
ACAGAGGAGGTCAGCAACCTAAAGACACTACCCAAGGGCCTGTCTGCCAG
CCTGCCTGACCTGGATTCTGAGAACTGGATTGAAGTGAAGAAGAGGCCTC
GGCCATCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCCAGATTTTCCCAC
CTGACCTCTCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAGGA
TCAGGATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGAGC
AGATGGATGGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCT
GACTATGAGATTGATGACAGGGATGTCAACAAGATCCTCATTGTCACCCA
GACACCACATTACATGCGCCGGCACCCAGGGGGGGACCGCACAGGCAACC
ACACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAAGGTCATTAATGAT
GGCCTCTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCTGA
GTATTCCCAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGA
TCAGCCGGGAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATCCC
AACCAGGAAGTTCCTCCTGGGCCACCTCGGTTCCAGCAAGTTCCTACGGA
TGCCCTGGCCAACAAGTTGTTTGGTGCTCCTGAGCCCTCCACCATCGCCC
GCTCTCTACCAACCACTGTCCCAGAGTCACCAAACTACCGCAACACCAGG
ACCCCTCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACATC
ACGGTTTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGC
CTCGAAAAAGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCAT
GTGGGCTGGGTGATGGATTCCCGTGAGCACAGGCCCCGTACTGCTTCCAT
CAGCTCCAGCCCCTCAGAAGGGACGCCTACAGTTGGCAGCTATGGCTGTA
CCCCTCAGTCATTGCCCAAGTTCCAGCATCCTTCCCATGAACTGCTCAAG
GAAAATGGCTTCACACAACACGTCTACCATAAGTATCGTAGGCGCTGCCT
TAATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACAC
TCTTCCGCTTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATG
TATGAGGAGTTCAAGCAGCTGGCTCTGGAGGACGCCAAAGAAGGCTACAG
ATATGGTTTGGAGTGCCTTTTTCGATACTACAGTTATGGCCTGGAAAAGA
AGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAAACGGTGAAGGAC
TATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTGAA
ATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACC
TCGGCAAATTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGT
GAGGAGGGCAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGG
TGAGGGCAGGAAGCGGTGCCCCTCCCAGTCTTCCAGCAGGCCTGCTGCCA
TGATCAGCCAACCCCCTACACCACCCACCGGCCAGCCTGTCCGGGAAGAT
GCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTGAGT
CGAC

Amino Acid Sequence

MYPYDVPDYAGSPEFAADRVVCLARRASPPSVHIAAAPEPGSPGRPGGGG
EGGTGLEACRARGGGVLRGTGGGGRTPRRPGLQSGGPSSPPAPLVGLGFT
AAASNWEGAAPRVNPDPSLQGLGPSAPEEGVAGALLAKVNPWTKNALPPV
LTTVNGQSPPEHSAPAKVVRAAVPKQRKGSKVGDFGDAINWPTPGEIAHK
SVQPQSHKPQPTRKLPPKKDMKEQEKGEGSDSKESPKTKSDESGEEKNGD
EDCQRGGQKKKGNKHKWVPLQIDMKPEVPREKLASRPTRPPEPRHIPANR
GEIKGSESATYVPVAPPTPAWQPEIKPEPAWHDQDETSSVKSDGAGGARA
SFRGRGRGRGRGRGRGRGGTRTHFDYQFGYRKFDGVEGPRTPKYMNNITY
YFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDADGF
LPITLIASFHRVQALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPL
PPIVDYSQTDFSQLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEV
SNLKTLPKGLSASLPDLDSENWIEVKKRPRPSPARPKKSEESRFSHLTSL
PQQLPSQQLMSKDQDEQEELDFLFDEEMEQMDGRKNTFTAWSDEESDYEI
DDRDVNKILIVTQTPHYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFY
YEQDLWAEKFEPEYSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEV
PPGPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNYRNTRTPRT
PRTPQLKDSSQTSRFYPVVKEGRTLDAKMPRKRKTRHSSNPPLESHVGWV
MDSREHRPRTASISSSPSEGTPTVGSYGCTPQSLPKFQHPSHELLKENGF
TQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEF
KQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAG
QLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGN
HKRHSVVAGGGGGEGRKRCPSQSSSRPAAMISQPPTPPTGQPVREDAKWT
SQHSNTQTLGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4424
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.