cDNA Clone GYS2 (mouse)

• Plasmid: pCMV5 Gys2 R588E
• Parent Plasmid: pCMV5
• Expressed: GYS2 R588E
• Genbank: NM_145572
• Species: Mouse

Sequence of Insert

GCCACCATGCTCAGAGGCCGCTCCTTGTCGGTGACATCCCTTGGTGGGCT
CCCTGTGTGGGAAGCTGAAAGACTCCCTGTGGAAGACTTACTCCTTTTTG
AAGTTTCTTGGGAGGTGACCAACAAAGTTGGGGGCATCTGTACTGTGATC
CAGACCAAGGCCAAAACGACAGCCGATGAGTGGGGAGAGAATTACTTCCT
GATAGGTCCGTACTTTGAGCATAATATGAAGACTCAAGTAGAACAATGTG
AGCCCACCAACGATGCTGTCAGAAAAGCTGTGGATGCGATGAATAAACAT
GGCTGCCAGGTGCATTTTGGAAGATGGCTCATAGAAGGGAGTCCCTACGT
GGTGCTCTTTGACATCAGCTCCTCAGCATGGAACCTGGACAGATGGAAGG
GTGACTTCTGGGAAGCTTGTGGCGTTGGCATCCCTCATCATGACCGAGAA
GCTAACGACATGCTCATATTTGGGTCTTTAACTGCCTGGTTCTTAAAGGA
GGTGACAGACCACGCAGACGGTAAACACGTCATTGCCCAATTCCATGAAT
GGCAGGCTGGGACTGGGCTGATCCTTTCTCGTGCCAGGAAACTCCCCATT
GCCACAGTATTTACAACCCATGCCACACTGCTTGGGCGTTATCTCTGTGC
AGCAAATATTGACTTCTACAACCAGCTTGACAAGTTCGACATAGACAAAG
AGGCCGGGGAGAGGCAGATATACCACCGCTACTGCATGGAGCGGGCATCC
GTGCACTGTGCGCACGTGTTCACCACAGTGTCGGAAATCACAGCCATCGA
GGCAGAGCACATGCTGAAGAGGAAGCCTGATGTAGTGACTCCAAATGGTT
TGAATGTTAAGAAGTTTTCTGCAGTGCATGAATTTCAAAATCTCCACGCC
ATGTACAAGGCCAGGATACAGGATTTCGTTCGAGGTCATTTCTATGGTCA
TCTGGACTTTGATCTTGAGAAGACTTTATTCCTCTTCATTGCTGGGAGAT
ATGAATTCTCAAACAAAGGAGCAGACATCTTCCTGGAGTCCTTATCCAGG
CTTAATTTCCTCCTGAGGATGCATAAGAGTAACGTCACCGTGGTAGTGTT
TTTCATCATGCCTGCCAAGACAAACAATTTCAACGTGGAAACCCTGAAGG
GCCAGGCAGTGCGGAAACAGCTGTGGGACACTGTGCATTGTTTGAAGGAG
AAGTTTGGGAAGAAACTCTATGACGGGTTATTAAGAGGAGAAATTCCTGA
CATGAATAGTATTTTGGATCGAGATGACTTAACAATTATGAAAAGGGCCA
TCTTTTCAACTCAGAGACAGTCTTTGCCTCCTGTGACCACTCACAATATG
ATCGATGATTCCACGGATCCCATCCTCAGCACCATTAGACGAATCGGACT
TTTCAACAACCGTGCAGACAGAGTCAAGGTGATTTTACACCCAGAATTCC
TGTCCTCCACCAGCCCCCTATTGCCCATGGATTATGAAGAGTTTGTTCGA
GGTTGTCACCTTGGGGTGTTTCCATCGTACTATGAACCCTGGGGTTACAC
ACCAGCTGAATGCACAGTGATGGGCATCCCCAGTGTGACTACAAACCTCT
CCGGTTTCGGGTGTTTCGTGCAGGAGCATGTGGCTGACCCTACTGCATAC
GGTATTTACATCGTGGACAGACGCTTCCGCTCTCCAGACGATTCTTGCAA
CCAACTGACTCAGTTCCTCTACGGGTTTTGTAAACAGTCACGCCGGCAAA
GGATCATTCAGAGGAACGAGACGGAGAGGCTCTCAGATCTCCTGGACTGG
AGATACCTGGGCAGATATTACCAGCATGCCAGACACCTGACACTGAGCAG
GGCTTTTCCAGACAAATTCCACCTAGAGCCCACATCACCGCCAATGACGG
ATGGCTTTAAGTATCCCAGGCCCTCCTCAGTACCACCTTCTCCGTCAGGA
TCCCAGGCCTCCAGTCCTCAGAGCAGTGATGCAGAAGATGAAGAAGATGA
GGATGAGAGGTATGATGAGGAAGAGGAGGCTGAGAGGGATCGGCTAAATA
TCAAGTCACCGTTTTCTCTGAACCACTTTCCAAAGGGGAAGAAAAAGCTT
CATGGAGAATATAAGAACTGAGCGGCCGCG

Amino Acid Sequence

MLRGRSLSVTSLGGLPVWEAERLPVEDLLLFEVSWEVTNKVGGICTVIQT
KAKTTADEWGENYFLIGPYFEHNMKTQVEQCEPTNDAVRKAVDAMNKHGC
QVHFGRWLIEGSPYVVLFDISSSAWNLDRWKGDFWEACGVGIPHHDREAN
DMLIFGSLTAWFLKEVTDHADGKHVIAQFHEWQAGTGLILSRARKLPIAT
VFTTHATLLGRYLCAANIDFYNQLDKFDIDKEAGERQIYHRYCMERASVH
CAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMY
KARIQDFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLN
FLLRMHKSNVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTVHCLKEKF
GKKLYDGLLRGEIPDMNSILDRDDLTIMKRAIFSTQRQSLPPVTTHNMID
DSTDPILSTIRRIGLFNNRADRVKVILHPEFLSSTSPLLPMDYEEFVRGC
HLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFVQEHVADPTAYGI
YIVDRRFRSPDDSCNQLTQFLYGFCKQSRRQRIIQRNETERLSDLLDWRY
LGRYYQHARHLTLSRAFPDKFHLEPTSPPMTDGFKYPRPSSVPPSPSGSQ
ASSPQSSDAEDEEDEDERYDEEEEAERDRLNIKSPFSLNHFPKGKKKLHG
EYKN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4420
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression.