cDNA Clone WNK2

• Plasmid: pGEX6P-1-WNK2 166-489
• Parent Plasmid: pCMV5
• Expressed: GST-WNK2 166-489
• Genbank: NM_006648
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGGGCGCA
CTCGCCGGGACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAG
GCCGTGGCCACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCT
GGGCCGCGGTTCCTTCAAGACGGTCTACAAGGGGCTGGACACGGAGACCT
GGGTGGAGGTGGCCTGGTGTGAGCTGCAGGACCGGAAGCTCACCAAGCTG
GAGCGGCAGCGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCA
CCCCAACATCGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCA
AGCGGTGCATTGTGCTGGTGACGGAGCTGATGACCTCAGGGACGCTGAAG
ACATACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGCAGCTG
GTGCCGGCAGATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCAC
CCATCATCCACCGAGACCTGAAATGTGACAATATTTTCATCACCGGACCA
ACTGGGTCTGTGAAGATTGGCGACTTGGGCCTGGCCACTCTGAAAAGAGC
GTCATTTGCCAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGA
TGTACGAGGAGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATG
TGCATGCTGGAGATGGCCACCTCGGAGTACCCCTACTCGGAGTGCCAGAA
TGCGGCCCAGATCTACCGCAAGGTCACCTGTGGTATCAAGCCGGCCAGCT
TTGAGAAAGTGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATC
TGCAAAAACAAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGC
CTTCTTCGCAGAGGACACAGGCGTGAGGGTGGAGCTCGCGGAGGAGGACC
ACGGCAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCCAAG
AAACTGAAGGGAAAGTGAGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGRTRRDEPEEEEDDEDDLK
AVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKL
ERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLK
TYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGP
TGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGM
CMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEKVHDPEIKEIIGECI
CKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPK
KLKGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4406
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.