cDNA Clone MAST2

• Plasmid: pEBG6P-1 MAST2
• Parent Plasmid: pEBG6P
• Expressed: GST-MAST2
• Genbank: NM_015112
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCTAAGCGGAGCCGCTGCCGCGACCGACCGCAGCCGCCGCCG
CCCGACCGCCGGGAGGATGGAGTTCAGCGGGCAGCGGAGCTGTCTCAGTC
TTTGCCGCCGCGCCGGCGAGCGCCGCCCGGGAGGCAGCGGCTGGAGGAGC
GGACGGGCCCCGCGGGGCCCGAGGGCAAGGAGCAGGATGTAGTAACTGGA
GTTAGTCCCCTGCTCTTCAGGAAACTCAGTAATCCTGACATATTTTCATC
CACTGGAAAAGTTAAACTTCAGCGACAACTGAGTCAGGATGATTGTAAGT
TATGGAGAGGAAACCTGGCCAGCTCTCTATCGGGTAAGCAGCTGCTCCCT
TTGTCCAGCAGTGTACATAGCAGTGTGGGACAGGTGACTTGGCAGTCGTC
AGGAGAAGCATCAAACCTGGTTCGAATGAGAAACCAGTCCCTTGGACAGT
CTGCACCTTCTCTTACTGCTGGCCTGAAGGAGTTGAGCCTTCCAAGAAGA
GGCAGCTTTTGTCGGACAAGTAACCGCAAGAGCTTGATTGTGACCTCTAG
CACATCACCTACACTACCACGGCCACACTCACCACTCCATGGCCACACAG
GTAACAGTCCTTTGGACAGCCCCCGGAATTTCTCTCCAAATGCACCTGCT
CACTTTTCTTTTGTTCCTGCCCGTAGGACTGATGGGCGGCGCTGGTCTTT
GGCCTCTTTGCCCTCTTCAGGATATGGAACTAACACTCCTAGCTCCACTG
TCTCATCATCATGCTCCTCACAGGAAAAGCTGCATCAGTTGCCTTTCCAG
CCTACAGCTGATGAGCTGCACTTTTTGACGAAGCATTTCAGCACAGAGAG
CGTACCAGATGAGGAAGGACGGCAGTCCCCAGCCATGCGGCCTCGCTCCC
GGAGCCTCAGTCCCGGACGATCCCCAGTATCCTTTGACAGTGAAATAATA
ATGATGAATCATGTTTACAAAGAAAGATTCCCAAAGGCCACCGCACAAAT
GGAAGAGCGACTAGCAGAGTTTATTTCCTCCAACACTCCAGACAGCGTGC
TGCCCTTGGCAGATGGAGCCCTGAGCTTTATTCATCATCAGGTGATTGAG
ATGGCCCGAGACTGCCTGGATAAATCTCGGAGTGGCCTCATTACATCACA
ATACTTCTACGAACTTCAAGAGAATTTGGAGAAACTTTTACAAGATGCTC
ATGAGCGCTCAGAGAGCTCAGAAGTGGCTTTTGTGATGCAGCTGGTGAAA
AAGCTGATGATTATCATTGCCCGCCCAGCACGTCTCCTGGAATGCCTGGA
GTTTGACCCTGAAGAGTTCTACCACCTTTTAGAAGCAGCTGAGGGCCACG
CCAAAGAGGGACAAGGGATTAAATGTGACATTCCCCGCTACATCGTTAGC
CAGCTGGGCCTCACCCGGGATCCCCTAGAAGAAATGGCCCAGTTGAGCAG
CTGTGACAGTCCTGACACTCCAGAGACAGATGATTCTATTGAGGGCCATG
GGGCATCTCTGCCATCTAAAAAGACACCCTCTGAAGAGGACTTCGAGACC
ATTAAGCTCATCAGCAATGGCGCCTATGGGGCTGTATTTCTGGTGCGGCA
CAAGTCCACCCGGCAGCGCTTTGCCATGAAGAAGATCAACAAGCAGAACC
TGATCCTACGGAACCAGATCCAGCAGGCCTTCGTGGAGCGTGATATACTG
ACTTTCGCTGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGATAC
CAAGCGCCACTTGTGCATGGTGATGGAGTACGTTGAAGGGGGAGACTGTG
CCACTCTGCTGAAGAATATTGGGGCCCTGCCTGTGGACATGGTGCGTCTA
TACTTTGCGGAAACTGTGCTGGCCCTGGAGTACTTACACAACTATGGCAT
CGTGCACCGTGACCTCAAGCCTGACAACCTCCTAATTACATCCATGGGGC
ACATCAAGCTCACGGACTTTGGACTGTCCAAAATGGGCCTCATGAGTCTG
ACAACGAACTTGTATGAGGGTCATATTGAAAAGGATGCCCGGGAATTCCT
GGACAAGCAGGTATGCGGGACCCCAGAATACATTGCGCCTGAGGTGATCC
TGCGCCAGGGCTATGGGAAGCCAGTGGACTGGTGGGCCATGGGCATTATC
CTGTATGAGTTCCTGGTGGGCTGCGTCCCTTTTTTTGGAGATACTCCGGA
GGAGCTCTTTGGGCAGGTGATCAGTGATGAGATTGTGTGGCCTGAGGGTG
ATGAGGCACTGCCCCCAGACGCCCAGGACCTCACCTCCAAACTGCTCCAC
CAGAACCCTCTGGAGAGACTTGGCACAGGCAGTGCCTATGAGGTGAAGCA
GCACCCATTCTTTACTGGTCTGGACTGGACAGGACTTCTCCGCCAGAAGG
CTGAATTTATTCCTCAGTTGGAGTCAGAGGATGATACTAGCTATTTTGAC
ACCCGCTCAGAGCGATACCACCACATGGACTCGGAGGATGAGGAAGAAGT
GAGTGAGGATGGCTGCCTTGAGATCCGCCAGTTCTCTTCCTGCTCTCCAA
GGTTCAACAAGGTGTACAGCAGCATGGAGCGGCTCTCACTGCTCGAGGAG
CGCCGGACACCACCCCCGACCAAGCGCAGCCTGAGTGAGGAGAAGGAGGA
CCATTCAGATGGCCTGGCAGGGCTCAAAGGCCGAGACCGGAGCTGGGTGA
TTGGCTCCCCTGAGATATTACGGAAGCGGCTGTCGGTGTCTGAGTCGTCC
CACACAGAGAGTGACTCAAGCCCTCCAATGACAGTGCGACGCCGCTGCTC
AGGCCTCCTGGATGCGCCTCGGTTCCCGGAGGGCCCTGAGGAGGCCAGCA
GCACCCTCAGGAGGCAACCACAGGAGGGTATATGGGTCCTGACACCCCCA
TCTGGAGAGGGGGTATCTGGGCCTGTCACTGAACACTCAGGGGAGCAGCG
GCCAAAGCTGGATGAGGAAGCTGTTGGCCGGAGCAGTGGTTCCAGTCCAG
CTATGGAGACCCGAGGCCGTGGGACCTCACAGCTGGCTGAGGGAGCCACA
GCCAAGGCCATCAGTGACCTGGCTGTGCGTAGGGCCCGCCACCGGCTGCT
CTCTGGGGACTCAACAGAGAAGCGCACTGCTCGCCCTGTCAACAAAGTGA
TCAAGTCCGCCTCAGCCACAGCCCTCTCACTCCTCATTCCTTCGGAACAC
CACACCTGCTCCCCGTTGGCCAGCCCCATGTCCCCACATTCTCAGTCGTC
CAACCCATCATCCCGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCC
TTGGCAGCATGAGGCCTCCCATCATCATCCACCGAGCTGGCAAGAAGTAT
GGCTTCACCCTGCGGGCCATTCGCGTCTACATGGGTGACTCCGATGTCTA
CACCGTGCACCATATGGTGTGGCACGTGGAGGATGGAGGTCCGGCCAGTG
AGGCAGGGCTTCGTCAAGGTGACCTCATCACCCATGTCAATGGGGAACCT
GTGCATGGCCTGGTGCACACGGAGGTGGTGGAGCTGATCCTGAAGAGTGG
AAACAAGGTGGCCATTTCAACAACTCCCCTGGAGAACACATCCATTAAAG
TGGGGCCAGCTCGGAAGGGCAGCTACAAGGCCAAGATGGCCCGAAGGAGC
AAGAGGAGCCGCGGCAAGGATGGGCAAGAAAGCAGAAAAAGGAGCTCCCT
GTTCCGCAAGATCACCAAGCAAGCATCCCTGCTCCACACCAGCCGCAGCC
TTTCTTCCCTTAACCGCTCCTTGTCATCAGGGGAGAGTGGGCCAGGCTCT
CCCACACACAGCCACAGCCTTTCCCCCCGATCTCCCACTCAAGGCTACCG
GGTGACCCCCGATGCTGTGCATTCAGTGGGAGGGAATTCATCACAGAGCA
GCTCCCCCAGCTCCAGCGTGCCCAGTTCCCCAGCCGGCTCTGGGCACACA
CGGCCCAGCTCCCTCCACGGTCTGGCACCCAAGCTCCAACGCCAGTACCG
CTCTCCACGGCGCAAGTCAGCAGGCAGCATCCCACTGTCACCACTGGCCC
ACACCCCTTCTCCCCCACCCCCAACAGCTTCACCTCAGCGGTCCCCATCG
CCCCTGTCTGGCCATGTAGCCCAGGCCTTTCCCACAAAGCTTCACTTGTC
ACCTCCCCTGGGCAGGCAACTCTCACGGCCCAAGAGTGCGGAGCCACCCC
GTTCACCACTACTCAAGAGGGTGCAGTCGGCTGAGAAACTGGCAGCAGCA
CTTGCCGCCTCTGAGAAGAAGCTAGCCACTTCTCGCAAGCACAGCCTTGA
CCTGCCCCACTCTGAACTAAAGAAGGAACTGCCGCCCAGGGAAGTGAGCC
CTCTGGAGGTAGTTGGAGCCAGGAGTGTGCTGTCTGGCAAGGGGGCCCTG
CCAGGGAAGGGGGTGCTGCAGCCTGCTCCCTCACGGGCCCTAGGCACCCT
CCGGCAGGACCGAGCCGAACGACGGGAGTCGCTGCAGAAGCAAGAAGCCA
TTCGTGAGGTGGACTCCTCAGAGGACGACACCGAGGAAGGGCCTGAGAAC
AGCCAGGGTGCACAGGAGCTGAGCTTGGCACCTCACCCAGAAGTGAGCCA
GAGTGTGGCCCCTAAAGGAGCAGGAGAGAGTGGGGAAGAGGATCCTTTCC
CGTCCAGAGGCCCTAGGAGCCTGGGCCCAATGGTCCCAAGCCTATTGACA
GGGATCACACTGGGGCCTCCCAGAATGGAAAGCCCCAGTGGTCCCCACAG
GAGGCTCGGGAGCCCACAAGCCATTGAGGAGGCTGCCAGCTCCTCCTCAG
CAGGCCCCAACCTAGGTCAGTCTGGAGCCACAGACCCCATCCCTCCTGAA
GGTTGCTGGAAGGCCCAGCACCTCCACACCCAGGCACTAACAGCACTTTC
TCCCAGCACTTCGGGACTCACCCCCACCAGCAGTTGCTCTCCTCCCAGCT
CCACCTCTGGGAAGCTGAGCATGTGGTCCTGGAAATCCCTTATTGAGGGC
CCAGACAGGGCATCCCCAAGCAGAAAGGCAACCATGGCAGGTGGGCTAGC
CAACCTCCAGGATTTGGAAACACAACTCCAGCCCAGCCTAAGAACCTGTC
TCCCAGGGAGCAGGGGAAGACACAGCCACCTAGTGCCCCCAGACTGGCCC
ATCCATCTTATGAGGATCCCAGCCAGGGCTGGCTATGGGAGTCTGAGTGT
GCACAAGCAGTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSKRSRCRDRPQPPP
PDRREDGVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTG
VSPLLFRKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLP
LSSSVHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRR
GSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPA
HFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQ
PTADELHFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEII
MMNHVYKERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIE
MARDCLDKSRSGLITSQYFYELQENLEKLLQDAHERSESSEVAFVMQLVK
KLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVS
QLGLTRDPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFET
IKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDIL
TFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRL
YFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSL
TTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGII
LYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLH
QNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFD
TRSERYHHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEE
RRTPPPTKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESS
HTESDSSPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPP
SGEGVSGPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGAT
AKAISDLAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEH
HTCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKY
GFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEP
VHGLVHTEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRS
KRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGS
PTHSHSLSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHT
RPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPS
PLSGHVAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAA
LAASEKKLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGAL
PGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPEN
SQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRGPRSLGPMVPSLLT
GITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPE
GCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEG
PDRASPSRKATMAGGLANLQDLETQLQPSLRTCLPGSRGRHSHLVPPDWP
IHLMRIPARAGYGSLSVHKQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4399
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.