cDNA Clone MAST2

• Plasmid: pGEX6P-1-MAST2
• Parent Plasmid: pGEX6P1
• Expressed: GST-MAST2
• Genbank: NM_015112
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTAAGCGGA
GCCGCTGCCGCGACCGACCGCAGCCGCCGCCGCCCGACCGCCGGGAGGAT
GGAGTTCAGCGGGCAGCGGAGCTGTCTCAGTCTTTGCCGCCGCGCCGGCG
AGCGCCGCCCGGGAGGCAGCGGCTGGAGGAGCGGACGGGCCCCGCGGGGC
CCGAGGGCAAGGAGCAGGATGTAGTAACTGGAGTTAGTCCCCTGCTCTTC
AGGAAACTCAGTAATCCTGACATATTTTCATCCACTGGAAAAGTTAAACT
TCAGCGACAACTGAGTCAGGATGATTGTAAGTTATGGAGAGGAAACCTGG
CCAGCTCTCTATCGGGTAAGCAGCTGCTCCCTTTGTCCAGCAGTGTACAT
AGCAGTGTGGGACAGGTGACTTGGCAGTCGTCAGGAGAAGCATCAAACCT
GGTTCGAATGAGAAACCAGTCCCTTGGACAGTCTGCACCTTCTCTTACTG
CTGGCCTGAAGGAGTTGAGCCTTCCAAGAAGAGGCAGCTTTTGTCGGACA
AGTAACCGCAAGAGCTTGATTGTGACCTCTAGCACATCACCTACACTACC
ACGGCCACACTCACCACTCCATGGCCACACAGGTAACAGTCCTTTGGACA
GCCCCCGGAATTTCTCTCCAAATGCACCTGCTCACTTTTCTTTTGTTCCT
GCCCGTAGGACTGATGGGCGGCGCTGGTCTTTGGCCTCTTTGCCCTCTTC
AGGATATGGAACTAACACTCCTAGCTCCACTGTCTCATCATCATGCTCCT
CACAGGAAAAGCTGCATCAGTTGCCTTTCCAGCCTACAGCTGATGAGCTG
CACTTTTTGACGAAGCATTTCAGCACAGAGAGCGTACCAGATGAGGAAGG
ACGGCAGTCCCCAGCCATGCGGCCTCGCTCCCGGAGCCTCAGTCCCGGAC
GATCCCCAGTATCCTTTGACAGTGAAATAATAATGATGAATCATGTTTAC
AAAGAAAGATTCCCAAAGGCCACCGCACAAATGGAAGAGCGACTAGCAGA
GTTTATTTCCTCCAACACTCCAGACAGCGTGCTGCCCTTGGCAGATGGAG
CCCTGAGCTTTATTCATCATCAGGTGATTGAGATGGCCCGAGACTGCCTG
GATAAATCTCGGAGTGGCCTCATTACATCACAATACTTCTACGAACTTCA
AGAGAATTTGGAGAAACTTTTACAAGATGCTCATGAGCGCTCAGAGAGCT
CAGAAGTGGCTTTTGTGATGCAGCTGGTGAAAAAGCTGATGATTATCATT
GCCCGCCCAGCACGTCTCCTGGAATGCCTGGAGTTTGACCCTGAAGAGTT
CTACCACCTTTTAGAAGCAGCTGAGGGCCACGCCAAAGAGGGACAAGGGA
TTAAATGTGACATTCCCCGCTACATCGTTAGCCAGCTGGGCCTCACCCGG
GATCCCCTAGAAGAAATGGCCCAGTTGAGCAGCTGTGACAGTCCTGACAC
TCCAGAGACAGATGATTCTATTGAGGGCCATGGGGCATCTCTGCCATCTA
AAAAGACACCCTCTGAAGAGGACTTCGAGACCATTAAGCTCATCAGCAAT
GGCGCCTATGGGGCTGTATTTCTGGTGCGGCACAAGTCCACCCGGCAGCG
CTTTGCCATGAAGAAGATCAACAAGCAGAACCTGATCCTACGGAACCAGA
TCCAGCAGGCCTTCGTGGAGCGTGATATACTGACTTTCGCTGAGAACCCC
TTTGTGGTCAGCATGTTCTGCTCCTTTGATACCAAGCGCCACTTGTGCAT
GGTGATGGAGTACGTTGAAGGGGGAGACTGTGCCACTCTGCTGAAGAATA
TTGGGGCCCTGCCTGTGGACATGGTGCGTCTATACTTTGCGGAAACTGTG
CTGGCCCTGGAGTACTTACACAACTATGGCATCGTGCACCGTGACCTCAA
GCCTGACAACCTCCTAATTACATCCATGGGGCACATCAAGCTCACGGACT
TTGGACTGTCCAAAATGGGCCTCATGAGTCTGACAACGAACTTGTATGAG
GGTCATATTGAAAAGGATGCCCGGGAATTCCTGGACAAGCAGGTATGCGG
GACCCCAGAATACATTGCGCCTGAGGTGATCCTGCGCCAGGGCTATGGGA
AGCCAGTGGACTGGTGGGCCATGGGCATTATCCTGTATGAGTTCCTGGTG
GGCTGCGTCCCTTTTTTTGGAGATACTCCGGAGGAGCTCTTTGGGCAGGT
GATCAGTGATGAGATTGTGTGGCCTGAGGGTGATGAGGCACTGCCCCCAG
ACGCCCAGGACCTCACCTCCAAACTGCTCCACCAGAACCCTCTGGAGAGA
CTTGGCACAGGCAGTGCCTATGAGGTGAAGCAGCACCCATTCTTTACTGG
TCTGGACTGGACAGGACTTCTCCGCCAGAAGGCTGAATTTATTCCTCAGT
TGGAGTCAGAGGATGATACTAGCTATTTTGACACCCGCTCAGAGCGATAC
CACCACATGGACTCGGAGGATGAGGAAGAAGTGAGTGAGGATGGCTGCCT
TGAGATCCGCCAGTTCTCTTCCTGCTCTCCAAGGTTCAACAAGGTGTACA
GCAGCATGGAGCGGCTCTCACTGCTCGAGGAGCGCCGGACACCACCCCCG
ACCAAGCGCAGCCTGAGTGAGGAGAAGGAGGACCATTCAGATGGCCTGGC
AGGGCTCAAAGGCCGAGACCGGAGCTGGGTGATTGGCTCCCCTGAGATAT
TACGGAAGCGGCTGTCGGTGTCTGAGTCGTCCCACACAGAGAGTGACTCA
AGCCCTCCAATGACAGTGCGACGCCGCTGCTCAGGCCTCCTGGATGCGCC
TCGGTTCCCGGAGGGCCCTGAGGAGGCCAGCAGCACCCTCAGGAGGCAAC
CACAGGAGGGTATATGGGTCCTGACACCCCCATCTGGAGAGGGGGTATCT
GGGCCTGTCACTGAACACTCAGGGGAGCAGCGGCCAAAGCTGGATGAGGA
AGCTGTTGGCCGGAGCAGTGGTTCCAGTCCAGCTATGGAGACCCGAGGCC
GTGGGACCTCACAGCTGGCTGAGGGAGCCACAGCCAAGGCCATCAGTGAC
CTGGCTGTGCGTAGGGCCCGCCACCGGCTGCTCTCTGGGGACTCAACAGA
GAAGCGCACTGCTCGCCCTGTCAACAAAGTGATCAAGTCCGCCTCAGCCA
CAGCCCTCTCACTCCTCATTCCTTCGGAACACCACACCTGCTCCCCGTTG
GCCAGCCCCATGTCCCCACATTCTCAGTCGTCCAACCCATCATCCCGGGA
CTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTTGGCAGCATGAGGCCTC
CCATCATCATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCC
ATTCGCGTCTACATGGGTGACTCCGATGTCTACACCGTGCACCATATGGT
GTGGCACGTGGAGGATGGAGGTCCGGCCAGTGAGGCAGGGCTTCGTCAAG
GTGACCTCATCACCCATGTCAATGGGGAACCTGTGCATGGCCTGGTGCAC
ACGGAGGTGGTGGAGCTGATCCTGAAGAGTGGAAACAAGGTGGCCATTTC
AACAACTCCCCTGGAGAACACATCCATTAAAGTGGGGCCAGCTCGGAAGG
GCAGCTACAAGGCCAAGATGGCCCGAAGGAGCAAGAGGAGCCGCGGCAAG
GATGGGCAAGAAAGCAGAAAAAGGAGCTCCCTGTTCCGCAAGATCACCAA
GCAAGCATCCCTGCTCCACACCAGCCGCAGCCTTTCTTCCCTTAACCGCT
CCTTGTCATCAGGGGAGAGTGGGCCAGGCTCTCCCACACACAGCCACAGC
CTTTCCCCCCGATCTCCCACTCAAGGCTACCGGGTGACCCCCGATGCTGT
GCATTCAGTGGGAGGGAATTCATCACAGAGCAGCTCCCCCAGCTCCAGCG
TGCCCAGTTCCCCAGCCGGCTCTGGGCACACACGGCCCAGCTCCCTCCAC
GGTCTGGCACCCAAGCTCCAACGCCAGTACCGCTCTCCACGGCGCAAGTC
AGCAGGCAGCATCCCACTGTCACCACTGGCCCACACCCCTTCTCCCCCAC
CCCCAACAGCTTCACCTCAGCGGTCCCCATCGCCCCTGTCTGGCCATGTA
GCCCAGGCCTTTCCCACAAAGCTTCACTTGTCACCTCCCCTGGGCAGGCA
ACTCTCACGGCCCAAGAGTGCGGAGCCACCCCGTTCACCACTACTCAAGA
GGGTGCAGTCGGCTGAGAAACTGGCAGCAGCACTTGCCGCCTCTGAGAAG
AAGCTAGCCACTTCTCGCAAGCACAGCCTTGACCTGCCCCACTCTGAACT
AAAGAAGGAACTGCCGCCCAGGGAAGTGAGCCCTCTGGAGGTAGTTGGAG
CCAGGAGTGTGCTGTCTGGCAAGGGGGCCCTGCCAGGGAAGGGGGTGCTG
CAGCCTGCTCCCTCACGGGCCCTAGGCACCCTCCGGCAGGACCGAGCCGA
ACGACGGGAGTCGCTGCAGAAGCAAGAAGCCATTCGTGAGGTGGACTCCT
CAGAGGACGACACCGAGGAAGGGCCTGAGAACAGCCAGGGTGCACAGGAG
CTGAGCTTGGCACCTCACCCAGAAGTGAGCCAGAGTGTGGCCCCTAAAGG
AGCAGGAGAGAGTGGGGAAGAGGATCCTTTCCCGTCCAGAGGCCCTAGGA
GCCTGGGCCCAATGGTCCCAAGCCTATTGACAGGGATCACACTGGGGCCT
CCCAGAATGGAAAGCCCCAGTGGTCCCCACAGGAGGCTCGGGAGCCCACA
AGCCATTGAGGAGGCTGCCAGCTCCTCCTCAGCAGGCCCCAACCTAGGTC
AGTCTGGAGCCACAGACCCCATCCCTCCTGAAGGTTGCTGGAAGGCCCAG
CACCTCCACACCCAGGCACTAACAGCACTTTCTCCCAGCACTTCGGGACT
CACCCCCACCAGCAGTTGCTCTCCTCCCAGCTCCACCTCTGGGAAGCTGA
GCATGTGGTCCTGGAAATCCCTTATTGAGGGCCCAGACAGGGCATCCCCA
AGCAGAAAGGCAACCATGGCAGGTGGGCTAGCCAACCTCCAGGATTTGGA
AACACAACTCCAGCCCAGCCTAAGAACCTGTCTCCCAGGGAGCAGGGGAA
GACACAGCCACCTAGTGCCCCCAGACTGGCCCATCCATCTTATGAGGATC
CCAGCCAGGGCTGGCTATGGGAGTCTGAGTGTGCACAAGCAGTGAGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKRSRCRDRPQPPPPDRRED
GVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTGVSPLLF
RKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLPLSSSVH
SSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRT
SNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVP
ARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADEL
HFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVY
KERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIEMARDCL
DKSRSGLITSQYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMIII
ARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTR
DPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFETIKLISN
GAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENP
FVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETV
LALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYE
GHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLV
GCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLHQNPLER
LGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERY
HHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEERRTPPP
TKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESSHTESDS
SPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPPSGEGVS
GPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGATAKAISD
LAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEHHTCSPL
ASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKYGFTLRA
IRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVH
TEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGK
DGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHS
LSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLH
GLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPSPLSGHV
AQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEK
KLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGALPGKGVL
QPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQE
LSLAPHPEVSQSVAPKGAGESGEEDPFPSRGPRSLGPMVPSLLTGITLGP
PRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQ
HLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASP
SRKATMAGGLANLQDLETQLQPSLRTCLPGSRGRHSHLVPPDWPIHLMRI
PARAGYGSLSVHKQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4398
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.