cDNA Clone MAST2

• Plasmid: pCMV5-HA MAST2
• Parent Plasmid: pCMV5
• Expressed: HA-MAST2
• Genbank: NM_015112
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCTAAGCGGAG
CCGCTGCCGCGACCGACCGCAGCCGCCGCCGCCCGACCGCCGGGAGGATG
GAGTTCAGCGGGCAGCGGAGCTGTCTCAGTCTTTGCCGCCGCGCCGGCGA
GCGCCGCCCGGGAGGCAGCGGCTGGAGGAGCGGACGGGCCCCGCGGGGCC
CGAGGGCAAGGAGCAGGATGTAGTAACTGGAGTTAGTCCCCTGCTCTTCA
GGAAACTCAGTAATCCTGACATATTTTCATCCACTGGAAAAGTTAAACTT
CAGCGACAACTGAGTCAGGATGATTGTAAGTTATGGAGAGGAAACCTGGC
CAGCTCTCTATCGGGTAAGCAGCTGCTCCCTTTGTCCAGCAGTGTACATA
GCAGTGTGGGACAGGTGACTTGGCAGTCGTCAGGAGAAGCATCAAACCTG
GTTCGAATGAGAAACCAGTCCCTTGGACAGTCTGCACCTTCTCTTACTGC
TGGCCTGAAGGAGTTGAGCCTTCCAAGAAGAGGCAGCTTTTGTCGGACAA
GTAACCGCAAGAGCTTGATTGTGACCTCTAGCACATCACCTACACTACCA
CGGCCACACTCACCACTCCATGGCCACACAGGTAACAGTCCTTTGGACAG
CCCCCGGAATTTCTCTCCAAATGCACCTGCTCACTTTTCTTTTGTTCCTG
CCCGTAGGACTGATGGGCGGCGCTGGTCTTTGGCCTCTTTGCCCTCTTCA
GGATATGGAACTAACACTCCTAGCTCCACTGTCTCATCATCATGCTCCTC
ACAGGAAAAGCTGCATCAGTTGCCTTTCCAGCCTACAGCTGATGAGCTGC
ACTTTTTGACGAAGCATTTCAGCACAGAGAGCGTACCAGATGAGGAAGGA
CGGCAGTCCCCAGCCATGCGGCCTCGCTCCCGGAGCCTCAGTCCCGGACG
ATCCCCAGTATCCTTTGACAGTGAAATAATAATGATGAATCATGTTTACA
AAGAAAGATTCCCAAAGGCCACCGCACAAATGGAAGAGCGACTAGCAGAG
TTTATTTCCTCCAACACTCCAGACAGCGTGCTGCCCTTGGCAGATGGAGC
CCTGAGCTTTATTCATCATCAGGTGATTGAGATGGCCCGAGACTGCCTGG
ATAAATCTCGGAGTGGCCTCATTACATCACAATACTTCTACGAACTTCAA
GAGAATTTGGAGAAACTTTTACAAGATGCTCATGAGCGCTCAGAGAGCTC
AGAAGTGGCTTTTGTGATGCAGCTGGTGAAAAAGCTGATGATTATCATTG
CCCGCCCAGCACGTCTCCTGGAATGCCTGGAGTTTGACCCTGAAGAGTTC
TACCACCTTTTAGAAGCAGCTGAGGGCCACGCCAAAGAGGGACAAGGGAT
TAAATGTGACATTCCCCGCTACATCGTTAGCCAGCTGGGCCTCACCCGGG
ATCCCCTAGAAGAAATGGCCCAGTTGAGCAGCTGTGACAGTCCTGACACT
CCAGAGACAGATGATTCTATTGAGGGCCATGGGGCATCTCTGCCATCTAA
AAAGACACCCTCTGAAGAGGACTTCGAGACCATTAAGCTCATCAGCAATG
GCGCCTATGGGGCTGTATTTCTGGTGCGGCACAAGTCCACCCGGCAGCGC
TTTGCCATGAAGAAGATCAACAAGCAGAACCTGATCCTACGGAACCAGAT
CCAGCAGGCCTTCGTGGAGCGTGATATACTGACTTTCGCTGAGAACCCCT
TTGTGGTCAGCATGTTCTGCTCCTTTGATACCAAGCGCCACTTGTGCATG
GTGATGGAGTACGTTGAAGGGGGAGACTGTGCCACTCTGCTGAAGAATAT
TGGGGCCCTGCCTGTGGACATGGTGCGTCTATACTTTGCGGAAACTGTGC
TGGCCCTGGAGTACTTACACAACTATGGCATCGTGCACCGTGACCTCAAG
CCTGACAACCTCCTAATTACATCCATGGGGCACATCAAGCTCACGGACTT
TGGACTGTCCAAAATGGGCCTCATGAGTCTGACAACGAACTTGTATGAGG
GTCATATTGAAAAGGATGCCCGGGAATTCCTGGACAAGCAGGTATGCGGG
ACCCCAGAATACATTGCGCCTGAGGTGATCCTGCGCCAGGGCTATGGGAA
GCCAGTGGACTGGTGGGCCATGGGCATTATCCTGTATGAGTTCCTGGTGG
GCTGCGTCCCTTTTTTTGGAGATACTCCGGAGGAGCTCTTTGGGCAGGTG
ATCAGTGATGAGATTGTGTGGCCTGAGGGTGATGAGGCACTGCCCCCAGA
CGCCCAGGACCTCACCTCCAAACTGCTCCACCAGAACCCTCTGGAGAGAC
TTGGCACAGGCAGTGCCTATGAGGTGAAGCAGCACCCATTCTTTACTGGT
CTGGACTGGACAGGACTTCTCCGCCAGAAGGCTGAATTTATTCCTCAGTT
GGAGTCAGAGGATGATACTAGCTATTTTGACACCCGCTCAGAGCGATACC
ACCACATGGACTCGGAGGATGAGGAAGAAGTGAGTGAGGATGGCTGCCTT
GAGATCCGCCAGTTCTCTTCCTGCTCTCCAAGGTTCAACAAGGTGTACAG
CAGCATGGAGCGGCTCTCACTGCTCGAGGAGCGCCGGACACCACCCCCGA
CCAAGCGCAGCCTGAGTGAGGAGAAGGAGGACCATTCAGATGGCCTGGCA
GGGCTCAAAGGCCGAGACCGGAGCTGGGTGATTGGCTCCCCTGAGATATT
ACGGAAGCGGCTGTCGGTGTCTGAGTCGTCCCACACAGAGAGTGACTCAA
GCCCTCCAATGACAGTGCGACGCCGCTGCTCAGGCCTCCTGGATGCGCCT
CGGTTCCCGGAGGGCCCTGAGGAGGCCAGCAGCACCCTCAGGAGGCAACC
ACAGGAGGGTATATGGGTCCTGACACCCCCATCTGGAGAGGGGGTATCTG
GGCCTGTCACTGAACACTCAGGGGAGCAGCGGCCAAAGCTGGATGAGGAA
GCTGTTGGCCGGAGCAGTGGTTCCAGTCCAGCTATGGAGACCCGAGGCCG
TGGGACCTCACAGCTGGCTGAGGGAGCCACAGCCAAGGCCATCAGTGACC
TGGCTGTGCGTAGGGCCCGCCACCGGCTGCTCTCTGGGGACTCAACAGAG
AAGCGCACTGCTCGCCCTGTCAACAAAGTGATCAAGTCCGCCTCAGCCAC
AGCCCTCTCACTCCTCATTCCTTCGGAACACCACACCTGCTCCCCGTTGG
CCAGCCCCATGTCCCCACATTCTCAGTCGTCCAACCCATCATCCCGGGAC
TCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTTGGCAGCATGAGGCCTCC
CATCATCATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCCA
TTCGCGTCTACATGGGTGACTCCGATGTCTACACCGTGCACCATATGGTG
TGGCACGTGGAGGATGGAGGTCCGGCCAGTGAGGCAGGGCTTCGTCAAGG
TGACCTCATCACCCATGTCAATGGGGAACCTGTGCATGGCCTGGTGCACA
CGGAGGTGGTGGAGCTGATCCTGAAGAGTGGAAACAAGGTGGCCATTTCA
ACAACTCCCCTGGAGAACACATCCATTAAAGTGGGGCCAGCTCGGAAGGG
CAGCTACAAGGCCAAGATGGCCCGAAGGAGCAAGAGGAGCCGCGGCAAGG
ATGGGCAAGAAAGCAGAAAAAGGAGCTCCCTGTTCCGCAAGATCACCAAG
CAAGCATCCCTGCTCCACACCAGCCGCAGCCTTTCTTCCCTTAACCGCTC
CTTGTCATCAGGGGAGAGTGGGCCAGGCTCTCCCACACACAGCCACAGCC
TTTCCCCCCGATCTCCCACTCAAGGCTACCGGGTGACCCCCGATGCTGTG
CATTCAGTGGGAGGGAATTCATCACAGAGCAGCTCCCCCAGCTCCAGCGT
GCCCAGTTCCCCAGCCGGCTCTGGGCACACACGGCCCAGCTCCCTCCACG
GTCTGGCACCCAAGCTCCAACGCCAGTACCGCTCTCCACGGCGCAAGTCA
GCAGGCAGCATCCCACTGTCACCACTGGCCCACACCCCTTCTCCCCCACC
CCCAACAGCTTCACCTCAGCGGTCCCCATCGCCCCTGTCTGGCCATGTAG
CCCAGGCCTTTCCCACAAAGCTTCACTTGTCACCTCCCCTGGGCAGGCAA
CTCTCACGGCCCAAGAGTGCGGAGCCACCCCGTTCACCACTACTCAAGAG
GGTGCAGTCGGCTGAGAAACTGGCAGCAGCACTTGCCGCCTCTGAGAAGA
AGCTAGCCACTTCTCGCAAGCACAGCCTTGACCTGCCCCACTCTGAACTA
AAGAAGGAACTGCCGCCCAGGGAAGTGAGCCCTCTGGAGGTAGTTGGAGC
CAGGAGTGTGCTGTCTGGCAAGGGGGCCCTGCCAGGGAAGGGGGTGCTGC
AGCCTGCTCCCTCACGGGCCCTAGGCACCCTCCGGCAGGACCGAGCCGAA
CGACGGGAGTCGCTGCAGAAGCAAGAAGCCATTCGTGAGGTGGACTCCTC
AGAGGACGACACCGAGGAAGGGCCTGAGAACAGCCAGGGTGCACAGGAGC
TGAGCTTGGCACCTCACCCAGAAGTGAGCCAGAGTGTGGCCCCTAAAGGA
GCAGGAGAGAGTGGGGAAGAGGATCCTTTCCCGTCCAGAGGCCCTAGGAG
CCTGGGCCCAATGGTCCCAAGCCTATTGACAGGGATCACACTGGGGCCTC
CCAGAATGGAAAGCCCCAGTGGTCCCCACAGGAGGCTCGGGAGCCCACAA
GCCATTGAGGAGGCTGCCAGCTCCTCCTCAGCAGGCCCCAACCTAGGTCA
GTCTGGAGCCACAGACCCCATCCCTCCTGAAGGTTGCTGGAAGGCCCAGC
ACCTCCACACCCAGGCACTAACAGCACTTTCTCCCAGCACTTCGGGACTC
ACCCCCACCAGCAGTTGCTCTCCTCCCAGCTCCACCTCTGGGAAGCTGAG
CATGTGGTCCTGGAAATCCCTTATTGAGGGCCCAGACAGGGCATCCCCAA
GCAGAAAGGCAACCATGGCAGGTGGGCTAGCCAACCTCCAGGATTTGGAA
ACACAACTCCAGCCCAGCCTAAGAACCTGTCTCCCAGGGAGCAGGGGAAG
ACACAGCCACCTAGTGCCCCCAGACTGGCCCATCCATCTTATGAGGATCC
CAGCCAGGGCTGGCTATGGGAGTCTGAGTGTGCACAAGCAGTGAGCGGCC
GCG

Amino Acid Sequence

MYPYDVPDYAGSKRSRCRDRPQPPPPDRREDGVQRAAELSQSLPPRRRAP
PGRQRLEERTGPAGPEGKEQDVVTGVSPLLFRKLSNPDIFSSTGKVKLQR
QLSQDDCKLWRGNLASSLSGKQLLPLSSSVHSSVGQVTWQSSGEASNLVR
MRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRP
HSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGY
GTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESVPDEEGRQ
SPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFI
SSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSQYFYELQEN
LEKLLQDAHERSESSEVAFVMQLVKKLMIIIARPARLLECLEFDPEEFYH
LLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSCDSPDTPE
TDDSIEGHGASLPSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFA
MKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFDTKRHLCMVM
EYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPD
NLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTP
EYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVIS
DEIVWPEGDEALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLD
WTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHMDSEDEEEVSEDGCLEI
RQFSSCSPRFNKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGL
KGRDRSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRRRCSGLLDAPRF
PEGPEEASSTLRRQPQEGIWVLTPPSGEGVSGPVTEHSGEQRPKLDEEAV
GRSSGSSPAMETRGRGTSQLAEGATAKAISDLAVRRARHRLLSGDSTEKR
TARPVNKVIKSASATALSLLIPSEHHTCSPLASPMSPHSQSSNPSSRDSS
PSRDFLPALGSMRPPIIIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWH
VEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVAISTT
PLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQA
SLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPDAVHS
VGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAG
SIPLSPLAHTPSPPPPTASPQRSPSPLSGHVAQAFPTKLHLSPPLGRQLS
RPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKHSLDLPHSELKK
ELPPREVSPLEVVGARSVLSGKGALPGKGVLQPAPSRALGTLRQDRAERR
ESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAG
ESGEEDPFPSRGPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAI
EEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTP
TSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLETQ
LQPSLRTCLPGSRGRHSHLVPPDWPIHLMRIPARAGYGSLSVHKQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4397
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.