cDNA Clone DYRK1B

• Plasmid: pEBG6P-1 DYRK1B isoform b
• Parent Plasmid: pEBG6P
• Expressed: GST-DYRK1B isoform b
• Genbank: NM_006483.2
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCA
GGGCCCCAGGAGCACACGCAGGTATTGCCTGATGTGCGGCTACTGCCTCG
GAGGCTGCCCCTGGCCTTCCGGGATGCAACCTCAGCCCCGCTGCGTAAGC
TCTCTGTGGACCTCATCAAGACCTACAAGCACATCAATGAGGTATACTAT
GCGAAGAAGAAGCGGCGGGCCCAGCAGGCGCCACCCCAGGATTCGAGCAA
CAAGAAGGAGAAGAAGGTCCTGAACCATGGTTATGATGACGACAACCATG
ACTACATCGTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGAC
TCGCTCATTGGCAAAGGCTCCTTTGGCCAGGTGGTGAAAGCCTATGATCA
TCAGACCCAGGAGCTTGTGGCCATCAAGATCATCAAGAACAAAAAGGCTT
TCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTGATGAACCAG
CATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTCAT
GTTCCGGAACCACCTGTGCCTGGTATTTGAGCTGCTGTCCTACAACCTGT
ACGACCTCCTGCGCAACACCCACTTCCGCGGCGTCTCGCTGAACCTGACC
CGGAAGCTGGCGCAGCAGCTCTGCACGGCACTGCTCTTTCTGGCCACGCC
TGAGCTCAGCATCATTCACTGCGACCTCAAGCCCGAAAACATCTTGCTGT
GCAACCCCAAGCGCAGCGCCATCAAGATTGTGGACTTCGGCAGCTCCTGC
CAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGCCGCTTCTACCGCTC
ACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGT
CCCTGGGCTGCATCCTTGTGGAGATGCACACCGGAGAGCCCCTCTTCAGT
GGCTCCAATGAGGTCGACCAGATGAACCGCATTGTGGAGGTGCTGGGCAT
CCCACCGGCCGCCATGCTGGACCAGGCGCCCAAGGCTCGCAAGTACTTTG
AACGGCTGCCTGGGGGTGGCTGGACCCTACGAAGGACGAAAGAACTCAGG
AAGGACCTGGTGCTGCGCATGCTGGAGTATGAGCCCGCCGCCCGCATCAG
CCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGAGG
CCACCAACACGGGCCCGGCAGGCAGCAGTGCCTCCACCTCGCCCGCGCCC
CTCGACACCTGCCCCTCTTCCAGCACCGCCAGCTCCATCTCCAGTTCTGG
AGGCTCCAGTGGCTCCTCCAGTGACAACCGGACCTACCGCTACAGCAACC
GATATTGTGGGGGCCCTGGGCCCCCTATCACAGACTGTGAGATGAACAGC
CCCCAGGTCCCACCCTCCCAGCCGCTGCGGCCCTGGGCAGGGGGTGATGT
GCCCCACAAGACACATCAAGCCCCTGCCTCTGCCTCGTCACTGCCTGGGA
CCGGGGCCCAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCATCA
CCAACCTCACCACCACCCCCGGAGCTGATGGATGTGAGCCTGGTGGGCGG
CCCTGCTGACTGCTCCCCACCTCACCCAGCGCCTGCCCCCCAGCACCCGG
CTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGTCCACCCCTCCCG
CCTCCTGATGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGTGT
ACCCCAGAGCACAGCAGCCAGCTCGTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAVPPGHGPFSGFP
GPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYY
AKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEID
SLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQ
HDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLT
RKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSC
QLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFS
GSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELR
KDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAP
LDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNS
PQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPS
PTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLP
PPDDPATLGPHLGLRGVPQSTAASS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4325
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.
• Low Temp: No