cDNA Clone DYRK1B

• Plasmid: pGEX6P-1-DYRK1B isoform b
• Parent Plasmid: pGEX6P1
• Expressed: GST-DYRK1B isoform b
• Genbank: NM_006483.2
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGCCGTCC
CACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACG
CAGGTATTGCCTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTT
CCGGGATGCAACCTCAGCCCCGCTGCGTAAGCTCTCTGTGGACCTCATCA
AGACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGG
GCCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGT
CCTGAACCATGGTTATGATGACGACAACCATGACTACATCGTGCGCAGTG
GCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCATTGGCAAAGGC
TCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGT
GGCCATCAAGATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGA
TTGAGCTGCGGCTGCTGGAGCTGATGAACCAGCATGACACGGAGATGAAG
TACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTG
CCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACA
CCCACTTCCGCGGCGTCTCGCTGAACCTGACCCGGAAGCTGGCGCAGCAG
CTCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCAGCATCATTCA
CTGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCG
CCATCAAGATTGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATC
TACCAGTATATCCAGAGCCGCTTCTACCGCTCACCTGAGGTGCTCCTGGG
CACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTTG
TGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGAC
CAGATGAACCGCATTGTGGAGGTGCTGGGCATCCCACCGGCCGCCATGCT
GGACCAGGCGCCCAAGGCTCGCAAGTACTTTGAACGGCTGCCTGGGGGTG
GCTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGACCTGGTGCTGCGC
ATGCTGGAGTATGAGCCCGCCGCCCGCATCAGCCCCCTGGGGGCTCTGCA
GCACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGG
CAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCT
TCCAGCACCGCCAGCTCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTC
CAGTGACAACCGGACCTACCGCTACAGCAACCGATATTGTGGGGGCCCTG
GGCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGTCCCACCCTCC
CAGCCGCTGCGGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCA
AGCCCCTGCCTCTGCCTCGTCACTGCCTGGGACCGGGGCCCAGTTACCCC
CCCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCACCACCACCC
CCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCC
ACCTCACCCAGCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGA
CTCGGATGACTGGAGGTCGTCCACCCCTCCCGCCTCCTGATGACCCTGCC
ACTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGAGCACAGCAGC
CAGCTCGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAVPPGHGPFSGFPGPQEHT
QVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRR
AQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKG
SFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMK
YYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQ
LCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRI
YQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVD
QMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDLVLR
MLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPS
SSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPS
QPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPP
PELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPA
TLGPHLGLRGVPQSTAASS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4324
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.
• Low Temp: No