cDNA Clone DYRK1B

• Plasmid: pCMV5-HA-1 DYRK1B isoform b
• Parent Plasmid: pCMV5
• Expressed: HA-DYRK1B isoform b
• Genbank: NM_006483.2
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCGCCGTCCC
ACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACGC
AGGTATTGCCTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTC
CGGGATGCAACCTCAGCCCCGCTGCGTAAGCTCTCTGTGGACCTCATCAA
GACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGGG
CCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGTC
CTGAACCATGGTTATGATGACGACAACCATGACTACATCGTGCGCAGTGG
CGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCATTGGCAAAGGCT
CCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTG
GCCATCAAGATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGAT
TGAGCTGCGGCTGCTGGAGCTGATGAACCAGCATGACACGGAGATGAAGT
ACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTGC
CTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACAC
CCACTTCCGCGGCGTCTCGCTGAACCTGACCCGGAAGCTGGCGCAGCAGC
TCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCAGCATCATTCAC
TGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCGC
CATCAAGATTGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATCT
ACCAGTATATCCAGAGCCGCTTCTACCGCTCACCTGAGGTGCTCCTGGGC
ACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTTGT
GGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGACC
AGATGAACCGCATTGTGGAGGTGCTGGGCATCCCACCGGCCGCCATGCTG
GACCAGGCGCCCAAGGCTCGCAAGTACTTTGAACGGCTGCCTGGGGGTGG
CTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGACCTGGTGCTGCGCA
TGCTGGAGTATGAGCCCGCCGCCCGCATCAGCCCCCTGGGGGCTCTGCAG
CACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGGC
AGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCTT
CCAGCACCGCCAGCTCCATCTCCAGTTCTGGAGGCTCCAGTGGCTCCTCC
AGTGACAACCGGACCTACCGCTACAGCAACCGATATTGTGGGGGCCCTGG
GCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGTCCCACCCTCCC
AGCCGCTGCGGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCAA
GCCCCTGCCTCTGCCTCGTCACTGCCTGGGACCGGGGCCCAGTTACCCCC
CCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCACCACCACCCC
CGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCCA
CCTCACCCAGCGCCTGCCCCCCAGCACCCGGCTGCCTCAGCCCTCCGGAC
TCGGATGACTGGAGGTCGTCCACCCCTCCCGCCTCCTGATGACCCTGCCA
CTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGAGCACAGCAGCC
AGCTCGTGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRD
ATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLN
HGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAI
KIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLV
FELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCD
LKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTP
YDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQ
APKARKYFERLPGGGWTLRRTKELRKDLVLRMLEYEPAARISPLGALQHG
FFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSD
NRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAP
ASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPH
PAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4323
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.
• Low Temp: No