cDNA Clone DYRK1A

• Plasmid: pCMV5-HA-3 DYRK1A
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-DYRK1A
• Genbank: NP¬¬_001387
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGATCCCCGGAA
TTCCCGGGTCGACCATACAGGAGGAGAGACTTCAGCATGCAAACCTTCAT
CTGTCCGGCTTGCACCGTCGTTCTCATTCCATGCTGCTGGCCTTCAGATG
GCTGGACAGATGCCCCACTCACACCAGTACAGTGACCGTCGCCAGCCGAA
CATAAGTGACCAGCAGGTGTCTGCCTTATCATATTCTGACCAGATTCAGC
AACCTCTAACTAACCAGGTGATGCCTGATATTGTCATGTTACAGAGGCGG
ATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTGAGAAAACTCTC
TGTGGACTTGATCAAAACATACAAGCATATTAATGAGGTTTACTATGCAA
AAAAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATTCCAGTCATAAG
AAGGAGCGGAAGGTTTACAATGATGGTTACGATGATGATAACTATGATTA
TATTGTAAAAAACGGGGAAAAGTGGATGGATCGGTATGAAATCGACTCCT
TAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCTTATGACAGAGTG
GAGCAAGAATGGGTCGCCATTAAAATCATCAAGAACAAGAAAGCGTTTCT
GAATCAAGCCCAGATAGAAGTGCGGCTGCTTGAGCTCATGAACAAACACG
ACACTGAAATGAAGTACTACATAGTGCATTTGAAACGCCACTTTATGTTT
CGAAACCATCTCTGTTTAGTGTTTGAAATGCTGTCCTATAATCTCTATGA
TTTGTTGAGAAACACCAACTTCCGAGGGGTCTCTTTGAACCTAACACGAA
AGTTTGCGCAACAGATGTGCACAGCATTGCTTTTTCTTGCGACTCCAGAA
CTTAGTATCATTCACTGTGACTTAAAGCCTGAAAATATCCTTCTTTGTAA
CCCCAAACGCAGTGCAATCAAGATAGTTGACTTTGGCAGTTCTTGTCAGT
TGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTTTATCGGTCTCCA
GAGGTGCTACTGGGAATGCCTTATGACCTTGCCATTGATATGTGGTCCCT
CGGGTGTATTTTGGTTGAAATGCACACTGGAGAACCTCTGTTCAGTGGTG
CCAATGAGGTAGATCAGATGAATAAAATAGTGGAAGTTCTGGGTATTCCA
CCTGCTCATATTCTTGACCAAGCACCAAAAGCAAGAAAGTTCTTTGAGAA
GTTGCCAGATGGCACTTGGAACTTAAAGAAGACCAAAGATGGAAAACGGG
AGTACAAACCACCAGGAACCCGTAAACTTCATAACATTCTTGGAGTGGAA
ACAGGAGGACCTGGTGGGCGACGTGCTGGGGAGTCAGGTCATACGGTCGC
TGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCTTGATTATGACC
CCAAAACTCGAATTCAACCTTATTATGCTCTGCAGCACAGTTTCTTCAAG
AAAACAGCTGATGAAGGTACAAATACAAGTAATAGTGTATCTACAAGCCC
CGCCATGGAGCAGTCTCAGTCTTCGGGCACCACCTCCAGTACATCGTCAA
GCTCAGGTGGCTCATCGGGGACAAGCAACAGTGGGAGAGCCCGGTCGGAT
CCGACGCACCAGCATCGGCACAGTGGTGGGCACTTCACAGCTGCCGTGCA
GGCCATGGACTGCGAGACACACAGTCCCCAGGTGCGTCAGCAATTTCCTG
CTCCTCTTGGTTGGTCAGGCACTGAAGCTCCTACACAGGTCACTGTTGAA
ACTCATCCTGTTCAAGAAACAACCTTTCATGTAGCCCCTCAACAGAATGC
ATTGCATCATCACCATGGTAACAGTTCCCATCACCATCACCACCACCACC
ACCATCACCACCACCATGGACAACAAGCCTTGGGTAACCGGACCAGGCCA
AGGGTCTACAATTCTCCAACGAATAGCTCCTCTACCCAAGATTCTATGGA
GGTTGGCCACAGTCACCACTCCATGACATCCCTGTCTTCCTCAACGACTT
CTTCCTCGACATCTTCCTCCTCTACTGGTAACCAAGGCAATCAGGCCTAC
CAGAATCGCCCAGTGGCTGCTAATACCTTGGACTTTGGACAGAATGGAGC
TATGGACGTTAATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTGGCA
TAGCTGGACATCCAACATACCAATTTTCTGCTAATACAGGTCCTGCACAT
TACATGACTGAAGGACATCTGACGATGAGGCAAGGGGCTGATAGAGAAGA
GTCCCCCATGACAGGAGTTTGTGTGCAACAGAGTCCTGTAGCTAGCTCGT
GAGTCGAC

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDHTGGETSACKPSSVRLAPSFSFHAAGLQMAG
QMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMP
QTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKE
RKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQ
EWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRN
HLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELS
IIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEV
LLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPA
HILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETG
GPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKT
ADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPT
HQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETH
PVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRV
YNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQN
RPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYM
TEGHLTMRQGADREESPMTGVCVQQSPVASS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4314
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Nucleotides 2-872 are from a mouse EST and mutated to human (A32G, S47N, P57S) and nucleotides 873-2292 are human.