cDNA Clone sec23A

• Plasmid: pGEX6P-1-sec23A FL T498A
• Parent Plasmid: pGEX6P1
• Expressed: GST-sec23A T498A FL
• Genbank: NP_006355
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCACAACCT
ATTTGGAATTCATTCAACAAAATGAAGAACGAGATGGAGTCCGATTTAGT
TGGAATGTTTGGCCATCAAGTCGACTGGAAGCTACAAGAATGGTTGTTCC
TGTGGCAGCCCTGTTTACACCACTGAAAGAGAGACCTGACTTACCACCTA
TTCAATATGAACCTGTTCTGTGTAGTAGGACCACTTGCCGTGCAGTTTTG
AATCCTTTATGTCAAGTGGATTATCGAGCAAAACTTTGGGCTTGCAACTT
TTGTTACCAAAGGAATCAGTTTCCACCTAGTTATGCTGGTATATCTGAAC
TGAATCAGCCTGCTGAACTTTTACCTCAGTTTTCTAGCATTGAATATGTA
GTTCTGCGTGGTCCTCAGATGCCTTTGATATTCCTCTATGTGGTTGATAC
TTGCATGGAAGATGAAGATTTACAAGCCCTGAAAGAATCCATGCAGATGT
CATTAAGTCTTTTACCACCTACAGCTTTGGTTGGACTTATTACTTTTGGG
AGAATGGTTCAGGTTCATGAACTTGGATGTGAAGGCATTTCAAAAAGCTA
TGTCTTCAGAGGAACAAAAGATTTGTCTGCCAAACAACTGCAGGAAATGC
TGGGGCTCTCTAAAGTACCACTTACTCAAGCAACACGTGGTCCTCAGGTA
CAGCAGCCACCTCCTTCCAACAGATTCTTACAACCAGTACAGAAAATAGA
CATGAATCTCACAGATCTTCTGGGAGAACTCCAGCGAGACCCTTGGCCTG
TACCACAGGGAAAGAGACCTTTGCGTTCCTCTGGGGTGGCACTTTCCATA
GCTGTAGGACTGCTGGAGTGTACTTTTCCCAACACTGGTGCTCGTATCAT
GATGTTCATTGGTGGTCCTGCTACTCAGGGGCCTGGAATGGTGGTTGGAG
ATGAGTTGAAGACACCTATAAGATCGTGGCATGACATTGACAAAGACAAT
GCCAAATATGTTAAAAAGGGAACTAAGCATTTTGAAGCATTGGCTAATCG
AGCTGCTACAACTGGCCATGTTATTGATATCTATGCGTGTGCATTAGATC
AGACAGGTCTCCTGGAGATGAAATGCTGTCCCAACCTTACTGGAGGATAC
ATGGTAATGGGTGATTCTTTCAATACTTCCTTATTCAAACAAACTTTTCA
AAGAGTCTTTACCAAAGACATGCATGGACAGTTTAAAATGGGCTTTGGTG
GTACGCTAGAAATAAAGACCTCAAGGGAAATAAAGATTTCAGGAGCTATT
GGACCCTGTGTGTCGCTCAATTCTAAAGGACCCTGTGTGTCTGAAAATGA
GATAGGAACAGGTGGCACATGTCAGTGGAAGATATGTGGACTTAGTCCCA
CTACAACCTTAGCCATATATTTTGAGGTTGTCAATCAGCATAATGCTCCA
ATTCCTCAAGGAGGGCGTGGTGCAATCCAGTTTGTGACTCAGTATCAGCA
TTCAAGTGGGCAGAGACGCATCCGAGTGACCGCCATTGCTAGGAACTGGG
CAGATGCTCAAACTCAAATCCAAAACATTGCTGCATCTTTTGACCAGGAG
GCAGCTGCCATTCTTATGGCCCGGCTAGCAATATATAGAGCAGAAACAGA
AGAAGGTCCAGATGTGCTTAGGTGGCTGGACAGACAGCTCATTCGACTGT
GTCAGAAATTTGGAGAATATCATAAAGATGACCCAAGTTCCTTCAGATTT
TCAGAAACTTTCTCCCTTTATCCACAGAGGAGAGACTTCCCTAAAACCTC
TGGTGAGATCCTGGAGAACCAGGAAAAGGCAAGGTCTGCTTGTCTTAAGT
TTATGTTTCATTTAAGAAGATCTTCTTTCCTGCAAGTTTTTAACAATAGT
CCTGATGAGAGTTCATATTATCGTCACCATTTTATGCGTCAAGATCTGAC
CCAGTCTCTAATTATGATTCAGCCTATCCTGTATGCGTATTCTTTTAGTG
GACCACCAGAGCCGGTTCTTCTTGATAGCAGTAGCATTCTTGCAGATCGT
ATTCTTCTCATGGACACATTCTTCCAGATTTTGATTTATCATGGTGAGAC
CATAGCACAGTGGCGGAAGTCAGGATACCAGGATATGCCTGAGTATGAAA
ATTTCCGCCACCTTCTGCAAGCCCCAGTGGATGATGCACAGGAAATTCTT
CACTCCAGATTTCCAATGCCAAGATACATTGACACTGAACATGGAGGCAG
CCAGGCCCGTTTCCTCCTTTCAAAAGTCAACCCTTCACAGACTCATAATA
ATATGTATGCCTGGGGGCAGGAGTCTGGAGCACCTATTCTTACAGATGAT
GTTAGTTTACAAGTGTTTATGGATCACTTGAAGAAACTTGCTGTGTCCAG
TGCTGCTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTTYLEFIQQNEERDGVRFS
WNVWPSSRLEATRMVVPVAALFTPLKERPDLPPIQYEPVLCSRTTCRAVL
NPLCQVDYRAKLWACNFCYQRNQFPPSYAGISELNQPAELLPQFSSIEYV
VLRGPQMPLIFLYVVDTCMEDEDLQALKESMQMSLSLLPPTALVGLITFG
RMVQVHELGCEGISKSYVFRGTKDLSAKQLQEMLGLSKVPLTQATRGPQV
QQPPPSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSI
AVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDIDKDN
AKYVKKGTKHFEALANRAATTGHVIDIYACALDQTGLLEMKCCPNLTGGY
MVMGDSFNTSLFKQTFQRVFTKDMHGQFKMGFGGTLEIKTSREIKISGAI
GPCVSLNSKGPCVSENEIGTGGTCQWKICGLSPTTTLAIYFEVVNQHNAP
IPQGGRGAIQFVTQYQHSSGQRRIRVTAIARNWADAQTQIQNIAASFDQE
AAAILMARLAIYRAETEEGPDVLRWLDRQLIRLCQKFGEYHKDDPSSFRF
SETFSLYPQRRDFPKTSGEILENQEKARSACLKFMFHLRRSSFLQVFNNS
PDESSYYRHHFMRQDLTQSLIMIQPILYAYSFSGPPEPVLLDSSSILADR
ILLMDTFFQILIYHGETIAQWRKSGYQDMPEYENFRHLLQAPVDDAQEIL
HSRFPMPRYIDTEHGGSQARFLLSKVNPSQTHNNMYAWGQESGAPILTDD
VSLQVFMDHLKKLAVSSAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4308
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Note that sec23A with this accession number is without aa 580-603.