cDNA Clone hn RNP K isoform b

• Plasmid: pGEX6P-1-hn RNP K S116D isoform b
• Parent Plasmid: pGEX6P1
• Expressed: GST-hn RNP K S116D isoform b
• Genbank: AAH14980
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGAAACTGAACAGCCAGAAGAAACCTTCCCTAACACTGAAACCAATGGT
GAATTTGGTAAACGCCCTGCAGAAGATATGGAAGAGGAACAAGCATTTAA
AAGATCTAGAAACACTGATGAGATGGTTGAATTACGCATTCTGCTTCAGA
GCAAGAATGCTGGGGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCT
CTCCGTACAGACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCC
CGAGCGCATATTGAGTATCAGTGCTGATATTGAAACAATTGGAGAAATTC
TGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCAGATCCC
ACTGCAACCAGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAA
TTACCAACACTATAAAGGAAGTGACTTTGACTGCGAGTTGAGGCTGTTGA
TTCATCAGAGTCTAGCAGGAGGAATTATTGGGGTCAAAGGTGCTAAAATC
AAAGAACTTCGAGAGAACACTCAAACCACCATCAAGCTTTTCCAGGAATG
CTGTCCTCATTCCACTGACAGAGTTGTTCTTATTGGAGGAAAACCCGATA
GGGTTGTAGAGTGCATAAAGATCATCCTTGATCTTATATCTGAGTCTCCC
ATCAAAGGACGTGCACAGCCTTATGATCCCAATTTTTACGATGAAACCTA
TGATTATGGTGGTTTTACAATGATGTTTGATGACCGTCGCGGACGCCCAG
TGGGATTTCCCATGCGGGGAAGAGGTGGTTTTGACAGAATGCCTCCTGGT
CGGGGTGGGCGTCCCATGCCTCCATCTAGAAGAGATTATGATGATATGAG
CCCTCGTCGAGGACCACCTCCCCCTCCTCCCGGACGAGGCGGCCGGGGTG
GTAGCAGAGCTCGGAATCTTCCTCTTCCTCCACCACCACCACCTAGAGGG
GGAGACCTCATGGCCTATGACAGAAGAGGGAGACCTGGAGACCGTTACGA
CGGCATGGTTGGTTTCAGTGCTGATGAAACTTGGGACTCTGCAATAGATA
CATGGAGCCCATCAGAATGGCAGATGGCTTATGAACCACAGGGTGGCTCC
GGATATGATTATTCCTATGCAGGGGGTCGTGGCTCATATGGTGATCTTGG
TGGACCTATTATTACTACACAAGTAACTATTCCCAAAGATTTGGCTGGAT
CTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATCCGTCATGAGTCG
GGAGCTTCGATCAAAATTGATGAGCCTTTAGAAGGATCCGAAGATCGGAT
CATTACCATTACAGGAACACAGGACCAGATACAGAATGCACAGTATTTGC
TGCAGAACAGTGTGAAGCAGTATTCTGGAAAGTTTTTCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFETEQPEETFPNTETNG
EFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKA
LRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPDP
TATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKI
KELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESP
IKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPG
RGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRG
GDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGS
GYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHES
GASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4303
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminus GST tag.