cDNA Clone SGK3

• Plasmid: pFBHTb delta PX SGK3 S486D
• Parent Plasmid: pFastBac HTb
• Expressed: His-delta PX SGK3 S486D
• Genbank: BC015326
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCCATGGACAGTCCAAAAC
ACCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTTCTCAGAAGCTA
CACTCTACCTCACAGAACATCAACCTGGGACCGTCTGGAAATCCTCATGC
CAAACCAACTGACTTTGATTTCTTAAAAGTTATTGGAAAAGGCAGCTTTG
GCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATTTTATGCTGTC
AAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATAT
TATGGCTGAACGTAATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGG
TTGGATTGCATTATTCCTTCCAAACAACTGAAAAGCTTTATTTTGTTCTG
GATTTTGTTAATGGAGGGGAGCTTTTTTTCCACTTACAAAGAGAACGGTC
CTTTCCTGAGCACAGAGCTAGGTTTTACGCTGCTGAAATTGCTAGTGCAT
TGGGTTACTTACATTCCATCAAAATAGTATACAGAGACTTGAAACCAGAA
AATATTCTTTTGGATTCAGTAGGACATGTTGTCTTAACAGATTTTGGGCT
TTGTAAAGAAGGAATTGCTATTTCTGACACCACTACCACATTTTGTGGGA
CACCAGAGTATCTTGCACCTGAAGTAATTAGAAAACAGCCCTATGACAAT
ACTGTAGATTGGTGGTGCCTTGGGGCTGTTCTGTATGAAATGCTGTATGG
ATTGCCTCCTTTTTATTGCCGAGATGTTGCTGAAATGTATGACAATATCC
TTCACAAACCCCTAAGTTTGAGGCCAGGAGTGAGTCTTACAGCCTGGTCC
ATTCTGGAAGAACTCCTAGAAAAAGACAGGCAAAATCGACTTGGTGCCAA
GGAAGACTTTCTTGAAATTCAGAATCATCCTTTTTTTGAATCACTCAGCT
GGGCTGACCTTGTACAAAAGAAGATTCCACCACCATTTAATCCTAATGTG
GCTGGACCAGATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAAC
AGTTCCATATTCTGTGTGTGTATCTTCTGACTATTCTATAGTGAATGCCA
GTGTATTGGAGGCAGATGATGCATTCGTTGGTTTCGATTATGCACCTCCT
TCAGAAGACTTATTTTTGTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDSPKHQSDPSEDEDERSSQKLHSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFDYAPPSEDLFL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4276
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express N terminal 6HIS fusion.