cDNA Clone PIK4CB

• Plasmid: pCMV5-HA-1 PIK4CB delta 316-330
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-PIK4CB delta 316-330
• Genbank: NP_002642
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCTAGATTCTT
GGAAGCTCGAAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTG
CCCCCTTGAAGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGGAATAAT
GGGGGGTCCCTGCTAAGTGTCATCACGGAGGGGGTCGGGGAACTATCAGT
GATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAAG
TCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCCACTG
GAGTTGGTCAATGGGGATGGTGTGGACAGTGAGATCCGTTGCCTAGATGA
TCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGGGGCCGCTGTGG
CCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAACAACTCAGCT
AAACAGTCTTGGCTGCTGAGGCTGTTTGAGTCAAAACTGTTTGACATCTC
CATGGCCATTTCATACCTGTATAACTCCAAGGAGCCTGGAGTACAAGCCT
ACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTAT
CTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGACGTGGGTGA
TGCCATTAAGCCCTACATAGTCCACCGTTGCCGCCAGAGCATTAACTTTT
CCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAGACATGCACATT
TCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCTGATCCTCTC
AGATGAGCTAAAGCCAGCTCACAGGAAGAGGGAGCTGCCCTCCTTGAGCC
CGGCCCCTGATACAGGGCTGTCTCCCTCCAAAAGGACTCACCAGCGCTCT
AAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAAC
AGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGCCTGTTCGACTGGCTC
CTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAGCGGCTGGCC
ACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCTC
CCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGCT
TTGACCACCACGTGGTCCGTGTACCCCACACACAGGCTGTTGTCCTCAAC
TCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGTGA
AAACTTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATTC
GGAGTACGAGGTCCGTAGAAAACTTGCCCGAATGTGGTATTACCCATGAG
CAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAACGATGATGA
GGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAAG
TGCATACCAACAGCTGTGACAACATCTCCCAGTTCTCTGTGGACAGCATC
ACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTGCAGCAGGGGACATCCG
CCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTCAAACGAG
ACCCAGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAAA
GTACGGCGGATCAGAGAGGGCTCCCCCTACGGCCATCTCCCCAATTGGCG
GCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGCAAGAGCTTC
TGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCGA
GTGCCCCTTTGGATCAAGCCATACAAGATTCTTGTGATTTCGGCTGATAG
TGGCATGATTGAACCAGTGGTCAATGCTGTGTCCATCCATCAGGTGAAGA
AACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAGCACGGCAGT
TACACCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTTG
TGCTGGGTACTGCTTGGTCTGCTACCTGCTGCAAGTCAAGGACAGACACA
ATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCACATCGACTTT
GGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACGTCAGC
CTTTAAGCTGACCACAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGCG
ACATGTTCAACTACTATAAGATGCTGATGCTGCAAGGGCTGATTGCCGCT
CGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCATGCAGCAAGG
TTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAAG
AGAGGTTCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGAG
CAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACTCTATGACGG
CTTCCAGTACCTCACCAACGGCATCATGTGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSRFLEARSLAVAMGDTVVEPAPLKPTSEPTSGPPGNNGG
SLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLEL
VNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQ
SWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLP
QLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHIST
QRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKS
DATASISLSSNLKRTASNPKVENEDEPVRLAPEREFIKSLMAIGKRLATL
PTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSK
DKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQR
AGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITS
QESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVR
RIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVP
LWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYT
TEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGF
ILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARK
HMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQM
VDGSMRSITTKLYDGFQYLTNGIM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA. Compare with protein sequence with AAH40300.