cDNA Clone DYRK1A

• Plasmid: pGEX6P-3-DYRK1A
• Parent Plasmid: pGEX6P3
• Expressed: GST-DYRK1A
• Genbank: NP_001387
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACCATACAGGAGGAGAGACTTCAGCATGCAAACCTTCATCT
GTCCGGCTTGCACCGTCGTTCTCATTCCATGCTGCTGGCCTTCAGATGGC
TGGACAGATGCCCCACTCACACCAGTACAGTGACCGTCGCCAGCCGAACA
TAAGTGACCAGCAGGTGTCTGCCTTATCATATTCTGACCAGATTCAGCAA
CCTCTAACTAACCAGGTGATGCCTGATATTGTCATGTTACAGAGGCGGAT
GCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTGAGAAAACTCTCTG
TGGACTTGATCAAAACATACAAGCATATTAATGAGGTTTACTATGCAAAA
AAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATTCCAGTCATAAGAA
GGAGCGGAAGGTTTACAATGATGGTTACGATGATGATAACTATGATTATA
TTGTAAAAAACGGGGAAAAGTGGATGGATCGGTATGAAATCGACTCCTTA
ATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCTTATGACAGAGTGGA
GCAAGAATGGGTCGCCATTAAAATCATCAAGAACAAGAAAGCGTTTCTGA
ATCAAGCCCAGATAGAAGTGCGGCTGCTTGAGCTCATGAACAAACACGAC
ACTGAAATGAAGTACTACATAGTGCATTTGAAACGCCACTTTATGTTTCG
AAACCATCTCTGTTTAGTGTTTGAAATGCTGTCCTATAATCTCTATGATT
TGTTGAGAAACACCAACTTCCGAGGGGTCTCTTTGAACCTAACACGAAAG
TTTGCGCAACAGATGTGCACAGCATTGCTTTTTCTTGCGACTCCAGAACT
TAGTATCATTCACTGTGACTTAAAGCCTGAAAATATCCTTCTTTGTAACC
CCAAACGCAGTGCAATCAAGATAGTTGACTTTGGCAGTTCTTGTCAGTTG
GGGCAGAGGATATACCAGTATATTCAGAGTCGCTTTTATCGGTCTCCAGA
GGTGCTACTGGGAATGCCTTATGACCTTGCCATTGATATGTGGTCCCTCG
GGTGTATTTTGGTTGAAATGCACACTGGAGAACCTCTGTTCAGTGGTGCC
AATGAGGTAGATCAGATGAATAAAATAGTGGAAGTTCTGGGTATTCCACC
TGCTCATATTCTTGACCAAGCACCAAAAGCAAGAAAGTTCTTTGAGAAGT
TGCCAGATGGCACTTGGAACTTAAAGAAGACCAAAGATGGAAAACGGGAG
TACAAACCACCAGGAACCCGTAAACTTCATAACATTCTTGGAGTGGAAAC
AGGAGGACCTGGTGGGCGACGTGCTGGGGAGTCAGGTCATACGGTCGCTG
ACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCTTGATTATGACCCC
AAAACTCGAATTCAACCTTATTATGCTCTGCAGCACAGTTTCTTCAAGAA
AACAGCTGATGAAGGTACAAATACAAGTAATAGTGTATCTACAAGCCCCG
CCATGGAGCAGTCTCAGTCTTCGGGCACCACCTCCAGTACATCGTCAAGC
TCAGGTGGCTCATCGGGGACAAGCAACAGTGGGAGAGCCCGGTCGGATCC
GACGCACCAGCATCGGCACAGTGGTGGGCACTTCACAGCTGCCGTGCAGG
CCATGGACTGCGAGACACACAGTCCCCAGGTGCGTCAGCAATTTCCTGCT
CCTCTTGGTTGGTCAGGCACTGAAGCTCCTACACAGGTCACTGTTGAAAC
TCATCCTGTTCAAGAAACAACCTTTCATGTAGCCCCTCAACAGAATGCAT
TGCATCATCACCATGGTAACAGTTCCCATCACCATCACCACCACCACCAC
CATCACCACCACCATGGACAACAAGCCTTGGGTAACCGGACCAGGCCAAG
GGTCTACAATTCTCCAACGAATAGCTCCTCTACCCAAGATTCTATGGAGG
TTGGCCACAGTCACCACTCCATGACATCCCTGTCTTCGTCAACGACTTCT
TCCTCGACATCTTCCTCCTCTACTGGTAACCAAGGCAATCAGGCCTACCA
GAATCGCCCAGTGGCTGCTAATACCTTGGACTTTGGACAGAATGGAGCTA
TGGACGTTAATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTGGCATA
GCTGGACATCCAACATACCAATTTTCTGCTAATACAGGTCCTGCACATTA
CATGACTGAAGGACATCTGACGATGAGGCAAGGGGCTGATAGAGAAGAGT
CCCCCATGACAGGAGTTTGTGTGCAACAGAGTCCTGTAGCTAGCTCGTGA
GTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDHTGGETSACKPSS
VRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQ
PLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAK
KKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSL
IGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHD
TEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRK
FAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQL
GQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGA
NEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKRE
YKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDP
KTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSS
SGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPA
PLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHH
HHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTS
SSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGI
AGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4247
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Nucleotides 2-872 are from a mouse EST and mutated to human (A32G, S47N, P57S) and nucleotides 873-2292 are human.