cDNA Clone TIFA

• Plasmid: pEBG6P-1 TIFA
• Parent Plasmid: pEBG6P
• Expressed: GST-TIFA
• Genbank: NM_052864
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACCAGTTTTGAAGATGCTGACACAGAAGAGACAGTAACT
TGTCTCCAGATGACGGTTTACCATCCTGGCCAGTTGCAGTGTGGAATATT
TCAGTCAATAAGTTTTAACAGAGAGAAACTCCCTTCCAGCGAAGTGGTGA
AATTTGGCCGAAATTCCAACATCTGTCATTATACTTTTCAGGACAAACAG
GTTTCCCGAGTTCAGTTTTCTCTGCAGCTGTTTAAAAAATTCAACAGCTC
AGTTCTCTCCTTTGAAATAAAAAATATGAGTAAAAAGACCAATCTGATCG
TGGACAGCAGAGAGCTGGGCTACCTAAATAAAATGGACCTGCCATACAGG
TGCATGGTCAGATTCGGAGAGTATCAGTTTCTGATGGAGAAGGAAGATGG
CGAGTCATTGGAATTTTTTGAGACTCAATTTATTTTATCTCCAAGATCAC
TCTTGCAAGAAAACAACTGGCCACCACACAGGCCCATACCGGAGTATGGC
ACTTACTCGCTCTGCTCCTCCCAAAGCAGTTCTCCGACAGAAATGGATGA
AAATGAGTCATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSFEDADTEETVT
CLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQ
VSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYR
CMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYG
TYSLCSSQSSSPTEMDENES*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4242
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.