cDNA Clone UBCH5a

• Plasmid: pEBG6P-1 UBE2D1/UBCH5a C85A
• Parent Plasmid: pEBG6P
• Expressed: GST-UBE2D1/UBCH5a C85A
• Genbank: NM_003338
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCGCGCTGAAGAGGATTCAGAAAGAATTGAGTGATCTACAG
CGCGATCCACCTGCTCACTGTTCAGCTGGACCTGTGGGAGATGACTTGTT
CCACTGGCAAGCCACTATTATGGGGCCTCCTGATAGCGCATATCAAGGTG
GAGTCTTCTTTCTCACTGTACATTTTCCGACAGATTATCCTTTTAAACCA
CCAAAGATTGCTTTCACAACAAAAATTTACCATCCAAACATAAACAGTAA
TGGAAGTATTGCTCTCGATATTCTGAGGTCACAATGGTCACCAGCTCTGA
CTGTATCAAAAGTTTTATTGTCCATATGTTCTCTACTTTGTGATCCTAAT
CCAGATGACCCCTTAGTACCAGATATTGCACAAATCTATAAATCAGACAA
AGAAAAATACAACAGACATGCAAGAGAATGGACTCAGAAATATGCAATGT
AAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSALKRIQKELSDLQ
RDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKP
PKIAFTTKIYHPNINSNGSIALDILRSQWSPALTVSKVLLSICSLLCDPN
PDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4222
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.