cDNA Clone PDK1

• Plasmid: pFBdual BirA 23aa STREP PDK1
• Parent Plasmid: pFastbacDual
• Expressed: His-BirA 23aa STREP PDK1
• Genbank: O15530
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGCTTCTTCTTTACGTCAAATTTTAGATTCTCAAAAAATGGAATGGCG
TTCTAATGCTGGTGGTTCTGAATTGGCGCTGGAAGTTCTGTTCCAGGGCC
CCTGGCATCATCACCATCACCATGGATCCCCGGAATTCGACGGCACTGCA
GCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCAGCATGCCCAGCCTCCGCC
GCAGCCTCGGAAGAAGCGGCCTGAGGACTTCAAGTTTGGGAAAATCCTTG
GGGAAGGCTCTTTTTCCACGGTTGTCCTGGCTCGAGAACTGGCAACCTCC
AGAGAATATGCGATTAAAATTCTGGAGAAGCGACATATCATAAAAGAGAA
CAAGGTCCCCTATGTAACCAGAGAGCGGGATGTCATGTCGCGCCTGGATC
ACCCCTTCTTTGTTAAGCTTTACTTCACATTTCAGGACGACGAGAAGCTG
TATTTCGGCCTTAGTTATGCCAAAAATGGAGAACTACTTAAATATATTCG
CAAAATCGGTTCATTCGATGAGACCTGTACCCGATTTTACACGGCTGAGA
TCGTGTCTGCTTTAGAGTACTTGCACGGCAAGGGCATCATTCACAGGGAC
CTTAAACCGGAAAACATTTTGTTAAATGAAGATATGCACATCCAGATCAC
AGATTTTGGAACAGCAAAAGTCTTATCCCCAGAGAGCAAACAAGCCAGGG
CCAACTCATTCGTGGGAACAGCGCAGTACGTTTCTCCAGAGCTGCTCACG
GAGAAGTCCGCCTGTAAGAGTTCAGACCTTTGGGCTCTTGGATGCATAAT
ATACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTGGAAACGAGTATC
TTATATTTCAGAAGATCATTAAGTTGGAATATGACTTTCCAGAAAAATTC
TTCCCTAAGGCAAGAGACCTCGTGGAGAAACTTTTGGTTTTAGATGCCAC
AAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGACCTCTTAAAGCAC
ACCCGTTCTTCGAGTCCGTCACGTGGGAGAACCTGCACCAGCAGACGCCT
CCGAAGCTCACCGCTTACCTGCCGGCTATGTCGGAAGACGACGAGGACTG
CTATGGCAATTATGACAATCTCCTGAGCCAGTTTGGCTGCATGCAGGTGT
CTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCCGACACGGGCCTGCCC
CAGAGGTCAGGCAGCAACATAGAGCAGTACATTCACGATCTGGACTCGAA
CTCCTTTGAACTGGACTTACAGTTTTCCGAAGATGAGAAGAGGTTGTTGT
TGGAGAAGCAGGCTGGCGGAAACCCTTGGCACCAGTTTGTAGAAAATAAT
TTAATACTAAAGATGGGCCCAGTGGATAAGCGGAAGGGTTTATTTGCAAG
ACGACGACAGCTGTTGCTCACAGAAGGACCACATTTATATTATGTGGATC
CTGTCAACAAAGTTCTGAAAGGTGAAATTCCTTGGTCACAAGAACTTCGA
CCAGAGGCCAAGAATTTTAAAACTTTCTTTGTCCACACGCCTAACAGGAC
GTATTATCTGATGGACCCCAGCGGGAACGCACACAAGTGGTGCAGGAAGA
TCCAGGAGGTTTGGAGGCAGCGATACCAGAGCCACCCGGACGCCGCTGTG
CAGTGAGTCGAC

Amino Acid Sequence

MASSLRQILDSQKMEWRSNAGGSELALEVLFQGPWHHHHHHGSPEFDGTA
AEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATS
REYAIKILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKL
YFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRD
LKPENILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLT
EKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKF
FPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTP
PKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLP
QRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENN
LILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELR
PEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAV
Q*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4197
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6H tag.