cDNA Clone PAK4

• Plasmid: pGEX6P-1-PAK4
• Parent Plasmid: pGEX6P1
• Expressed: GST-PAK4
• Genbank: O96013
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTTTGGGA
AGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGC
GTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCG
CCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCG
TCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATC
GTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGA
CGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACA
GCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAG
CCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCG
GTTCGCCGGTCACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGG
CGGGGCCAGAGAAGAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCC
CAGGAGTCCTCCCGGGACAAACGCCCCCTCTCCGGGCCTGATGTCGGCAC
CCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCAGCTGGCCGGC
CCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCGGGGTGCC
CAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCT
GGCCATCCCCCAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCC
GAGGTGCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAG
CTGGCCCCTCCAGCCTGCACCCCCGCCGCCCCTGCTGTTCCTGGGCCCCC
TGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGT
TCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTAC
CTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCAT
CGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACC
TGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATG
AGGGACTACCAGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGT
GGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCA
CCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTG
TGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCAT
CCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGG
TGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCC
CGAAGGAAGTCGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCAT
CTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAA
TGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCC
CTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAA
CCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGG
TGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCA
TTCCTGGCCAAGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCA
GAACCGCACCAGATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFGKRKKRVEISAPSNFEHR
VHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTI
VRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEE
PATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGP
QESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGA
QGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQ
LAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSY
LDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIM
RDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAV
CLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVP
RRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPP
LKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHP
FLAKAGPPASIVPLMRQNRTR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4186
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.