cDNA Clone Aurora A

• Synonyms: AURKA
• Plasmid: pET28a(+) Aurora A
• Parent Plasmid: pET28a original
• Expressed: His-Aurora A
• Genbank: BC027464
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGGCTACAGCT
CCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCA
GAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCTTGTGTCCTT
CAAATTCTTCCCAGCGCGTTCCTTTGCAAGCACAAAAGCTTGTCTCCAGT
CACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGCAACCAGTGT
ACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGC
CCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGGCATCAAAA
CAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGA
AATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTATTTGGCAA
GAGAACAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCT
CAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGAAGTAGAAAT
ACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCC
ATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCACTTGGAACA
GTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGC
TACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTCATTCGAAGA
GAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCT
GGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGCTCCATCTTC
CAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGA
TTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCCTTGGAGTT
CTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATA
CCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACATTCCCTGACT
TTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAAT
CCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCCCTGGATCAC
AGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCA
AACAGTCTTAGGTCGAC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSDRSKENCISGPVKATA
PVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSS
HKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASK
QKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREQQSKFILALKVLFKA
QLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGT
VYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSA
GELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGV
LCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHN
PSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal 6H.