cDNA Clone Lambda phosphatase

• Plasmid: pGEX6P-1-Lambda phosphatase Y3-A221 6HIS
• Parent Plasmid: pGEX6P1
• Expressed: GST-Lambda phosphatase Y3-A221 6HIS
• Genbank: AAA96594.1 (NP_040641.1)
• Tag: GST
• Species: Bacteriophage lambda

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTATTACG
AAAAAATTGATGGCAGCAAATACCGAAATATTTGGGTAGTTGGCGATCTG
CACGGATGCTACACGAACCTGATGAACAAACTGGATACGATTGGATTCGA
CAACAAAAAAGACCTGCTTATCTCGGTGGGCGATTTGGTTGATCGTGGTG
CAGAGAACGTTGAATGCCTGGAATTAATCACATTCCCCTGGTTCAGAGCT
GTACGTGGAAACCATGAGCAAATGATGATTGATGGCTTATCAGAGCGTGG
AAACGTTAATCACTGGCTGCTTAATGGCGGTGGCTGGTTCTTTAATCTCG
ATTACGACAAAGAAATTCTGGCTAAAGCTCTTGCCCATAAAGCAGATGAA
CTTCCGTTAATCATCGAACTGGTGAGCAAAGATAAAAAATATGTTATCTG
CCACGCCGATTATCCCTTTGACGAATACGAGTTTGGAAAGCCAGTTGATC
ATCAGCAGGTAATCTGGAACCGCGAACGAATCAGCAACTCACAAAACGGG
ATCGTGAAAGAAATCAAAGGCGCGGACACGTTCATCTTTGGTCATACGCC
AGCAGTGAAACCACTCAAGTTTGCCAACCAAATGTATATCGATACCGGCG
CAGTGTTCTGCGGAAACCTAACATTGATTCAGGTACAGGGAGAAGGCGCA
CACCATCACCATCACCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYYEKIDGSKYRNIWVVGDL
HGCYTNLMNKLDTIGFDNKKDLLISVGDLVDRGAENVECLELITFPWFRA
VRGNHEQMMIDGLSERGNVNHWLLNGGGWFFNLDYDKEILAKALAHKADE
LPLIIELVSKDKKYVICHADYPFDEYEFGKPVDHQQVIWNRERISNSQNG
IVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA
HHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4170
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST and C terminal 6H.
• Low Temp: No