cDNA Clone CDKL3

• Plasmid: pGEX6P-3-CDKL3 (SalI)
• Parent Plasmid: pGEX6P3
• Expressed: GST-CDKL3 (SalI)
• Genbank: BC041799/AAH41799
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACGAGATGTATGAAACCCTTGGAAAAGTGGGAGAGGGAAGT
TACGGAACAGTCATGAAATGTAAACATAAGAATACTGGGCAGATAGTGTC
CATTAAGATATTTTATGAGAGACCAGAACAATCTGTCAACAAAATTGCGA
TGAGAGAAATAAAGTTTCTAAAGCAATTTCATCACGAAAACCTGGTCAAT
CTGATTGAAGTTTTTAGACAGAAAAAGAAAATTCATTTGGTATTTGAATT
TATTGACCACACAGTATTAGATGAGTTACAACATTATTGTCATGGACTAG
AGAGTAAGCGACTTAGAAAATACCTCTTCCAGATCCTTCGAGCAATTGAC
TATCTTCACAGTAATAATATCATTCATCGAGATATAAAACCTGAGAATAT
TTTAGTATCCCAGTCAGGAATTACTAAGCTCTGTGATTTTGGTTTTGCAC
GAACACTAGCAGCTCCTGGGGACATTTATACGGACTATGTGGCCACACGC
TGGTATAGAGCTCCCGAATTAGTATTAAAAGATACTTCTTATGGAAAACC
TGTGGATATCTGGGCTTTGGGCTGTATGATCATTGAGATGGCCACTGGAA
ATCCCTATCTTCCTAGTAGTTCTGATTTGGATTTACTCCATAAAATTGTT
TTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATATCTTTTCCAAGAG
CCCCATTTTTGCTGGGGTAGTTCTTCCTCAAGTTCAACACCCCAAAAATG
CAAGAAAAAAATATCCAAAGCTTAATGGATTGTTGGCAGATATAGTTCAT
GCTTGTTTACAAATTGATCCTGCTGACAGGATATCATCTAGTGATCTTTT
GCATCATGAGTATTTTACTAGAGATGGATTTATTGAAAAATTCATGCCAG
AACTGAAAGCTAAATTACTGCAGGAAGCAAAAGTCAATTCATTAATAAAG
CCAAAAGAGAGTTCTAAAGAAAATGAACTCAGGAAAGATGAAAGAAAAAC
AGTTTATACCAATACACTGCTAAGTAGTTCAGTTTTGGGAAAGGAAATAG
AAAAAGAGAAAAAGCCCAAGGAGATCAAAGTCAGAGTTATTAAAGTCAAA
GGAGGAAGAGGAGATATCTCAGAACCAAAAAAGAAAGAGTATGAAGGTGG
ACTTGGTCAACAGGATGCAAATGAAAATGTTCATCCTATGTCTCCAGATA
CAAAACTTGTAACCATTGAACCACCAAACCCTATCAATCCCAGCACTAAC
TGTAATGGCTTGAAAGAAAATCCACATTGCGGAGGTTCTGTGACAATGCC
ACCCATCAATCTAACTAACAGTAATTTGATGGCTGCAAATCTCAGTTCAA
ATCTCTTTCACCCCAGTGTGAGGTTAACTGAAAGAGCAAAAAAGAGACGC
ACTTCTTCACAATCTATTGGACAAGTTATGCCTAATAGCAGGCAAGAGGA
TCCAGGTCCTATTCAAAGCCAAATGGAGAAGGGTATATTTAATGAGCGAA
CAGGTCACAGTGACCAAATGGCAAATGAGAACAAAAGGAAGCTGAATTTT
TCCAGATCTGACAGGAAAGAATTCCATTTTCCAGAATTGCCTGTCACAAT
ACAGTCAAAAGATACAAAAGGAATGGAAGTTAAACAGATAAAAATGCTGA
AGAGGGAGTCAAAGAAAACAGAGTCATCTAAGATACCAACTTTACTTAAC
GTGGATCAAAATCAAGAAAAACAAGAGGGTGGAGATGGCCATTGCGAGGG
GAAGAATTTGAAGAGAAACAGGTTTTTTTTCTGGTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDEMYETLGKVGEGS
YGTVMKCKHKNTGQIVSIKIFYERPEQSVNKIAMREIKFLKQFHHENLVN
LIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAID
YLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDYVATR
WYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIV
LKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVH
ACLQIDPADRISSSDLLHHEYFTRDGFIEKFMPELKAKLLQEAKVNSLIK
PKESSKENELRKDERKTVYTNTLLSSSVLGKEIEKEKKPKEIKVRVIKVK
GGRGDISEPKKKEYEGGLGQQDANENVHPMSPDTKLVTIEPPNPINPSTN
CNGLKENPHCGGSVTMPPINLTNSNLMAANLSSNLFHPSVRLTERAKKRR
TSSQSIGQVMPNSRQEDPGPIQSQMEKGIFNERTGHSDQMANENKRKLNF
SRSDRKEFHFPELPVTIQSKDTKGMEVKQIKMLKRESKKTESSKIPTLLN
VDQNQEKQEGGDGHCEGKNLKRNRFFFW*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4149
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.