cDNA Clone CDKL3

• Plasmid: pCMV5-HA CDKL3 (Insert (Bam/Sal)lig into vector KpnI/SalI)
• Parent Plasmid: pCMV5
• Expressed: HA-CDKL3
• Genbank: BC041799/AAH41799
• Tag: HA

Sequence of Insert

GGTACCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAGATGTA
TGAAACCCTTGGAAAAGTGGGAGAGGGAAGTTACGGAACAGTCATGAAAT
GTAAACATAAGAATACTGGGCAGATAGTGGCCATTAAGATATTTTATGAG
AGACCAGAACAATCTGTCAACAAAATTGCGATGAGAGAAATAAAGTTTCT
AAAGCAATTTCATCACGAAAACCTGGTCAATCTGATTGAAGTTTTTAGAC
AGAAAAAGAAAATTCATTTGGTATTTGAATTTATTGACCACACAGTATTA
GATGAGTTACAACATTATTGTCATGGACTAGAGAGTAAGCGACTTAGAAA
ATACCTCTTCCAGATCCTTCGAGCAATTGACTATCTTCACAGTAATAATA
TCATTCATCGAGATATAAAACCTGAGAATATTTTAGTATCCCAGTCAGGA
ATTACTAAGCTCTGTGATTTTGGTTTTGCACGAACACTAGCAGCTCCTGG
GGACATTTATACGGACTATGTGGCCACACGCTGGTATAGAGCTCCCGAAT
TAGTATTAAAAGATACTTCTTATGGAAAACCTGTGGATATCTGGGCTTTG
GGCTGTATGATCATTGAGATGGCCACTGGAAATCCCTATCTTCCTAGTAG
TTCTGATTTGGATTTACTCCATAAAATTGTTTTGAAAGTGGGCAATTTGT
CACCTCACTTGCAGAATATCTTTTCCAAGAGCCCCATTTTTGCTGGGGTA
GTTCTTCCTCAAGTTCAACACCCCAAAAATGCAAGAAAAAAATATCCAAA
GCTTAATGGATTGTTGGCAGATATAGTTCATGCTTGTTTACAAATTGATC
CTGCTGACAGGATATCATCTAGTGATCTTTTGCATCATGAGTATTTTACT
AGAGATGGATTTATTGAAAAATTCATGCCAGAACTGAAAGCTAAATTACT
GCAGGAAGCAAAAGTCAATTCATTAATAAAGCCAAAAGAGAGTTCTAAAG
AAAATGAACTCAGGAAAGATGAAAGAAAAACAGTTTATACCAATACACTG
CTAAGTAGTTCAGTTTTGGGAAAGGAAATAGAAAAAGAGAAAAAGCCCAA
GGAGATCAAAGTCAGAGTTATTAAAGTCAAAGGAGGAAGAGGAGATATCT
CAGAACCAAAAAAGAAAGAGTATGAAGGTGGACTTGGTCAACAGGATGCA
AATGAAAATGTTCATCCTATGTCTCCAGATACAAAACTTGTAACCATTGA
ACCACCAAACCCTATCAATCCCAGCACTAACTGTAATGGCTTGAAAGAAA
ATCCACATTGCGGAGGTTCTGTGACAATGCCACCCATCAATCTAACTAAC
AGTAATTTGATGGCTGCAAATCTCAGTTCAAATCTCTTTCACCCCAGTGT
GAGGTTAACTGAAAGAGCAAAAAAGAGACGCACTTCTTCACAATCTATTG
GACAAGTTATGCCTAATAGCAGGCAAGAGGATCCAGGTCCTATTCAAAGC
CAAATGGAGAAGGGTATATTTAATGAGCGAACAGGTCACAGTGACCAAAT
GGCAAATGAGAACAAAAGGAAGCTGAATTTTTCCAGATCTGACAGGAAAG
AATTCCATTTTCCAGAATTGCCTGTCACAATACAGTCAAAAGATACAAAA
GGAATGGAAGTTAAACAGATAAAAATGCTGAAGAGGGAGTCAAAGAAAAC
AGAGTCATCTAAGATACCAACTTTACTTAACGTGGATCAAAATCAAGAAA
AACAAGAGGGTGGAGATGGCCATTGCGAGGGGAAGAATTTGAAGAGAAAC
AGGTTTTTTTTCTGGTAGGTCGAC

Amino Acid Sequence

MYPYDVPDYAEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQ
SVNKIAMREIKFLKQFHHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQ
HYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITKL
CDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMI
IEMATGNPYLPSSSDLDLLHKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQ
VQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSDLLHHEYFTRDGF
IEKFMPELKAKLLQEAKVNSLIKPKESSKENELRKDERKTVYTNTLLSSS
VLGKEIEKEKKPKEIKVRVIKVKGGRGDISEPKKKEYEGGLGQQDANENV
HPMSPDTKLVTIEPPNPINPSTNCNGLKENPHCGGSVTMPPINLTNSNLM
AANLSSNLFHPSVRLTERAKKRRTSSQSIGQVMPNSRQEDPGPIQSQMEK
GIFNERTGHSDQMANENKRKLNFSRSDRKEFHFPELPVTIQSKDTKGMEV
KQIKMLKRESKKTESSKIPTLLNVDQNQEKQEGGDGHCEGKNLKRNRFFF
W*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4145
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA.