cDNA Clone CDKL3

• Plasmid: pEBG6P-1 CDKL3
• Parent Plasmid: pEBG6P
• Expressed: GST-CDKL3
• Genbank: BC041799/AAH41799
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCGAGATGTATGAAACCCTTGGAAAAGTG
GGAGAGGGAAGTTACGGAACAGTCATGAAATGTAAACATAAGAATACTGG
GCAGATAGTGGCCATTAAGATATTTTATGAGAGACCAGAACAATCTGTCA
ACAAAATTGCGATGAGAGAAATAAAGTTTCTAAAGCAATTTCATCACGAA
AACCTGGTCAATCTGATTGAAGTTTTTAGACAGAAAAAGAAAATTCATTT
GGTATTTGAATTTATTGACCACACAGTATTAGATGAGTTACAACATTATT
GTCATGGACTAGAGAGTAAGCGACTTAGAAAATACCTCTTCCAGATCCTT
CGAGCAATTGACTATCTTCACAGTAATAATATCATTCATCGAGATATAAA
ACCTGAGAATATTTTAGTATCCCAGTCAGGAATTACTAAGCTCTGTGATT
TTGGTTTTGCACGAACACTAGCAGCTCCTGGGGACATTTATACGGACTAT
GTGGCCACACGCTGGTATAGAGCTCCCGAATTAGTATTAAAAGATACTTC
TTATGGAAAACCTGTGGATATCTGGGCTTTGGGCTGTATGATCATTGAGA
TGGCCACTGGAAATCCCTATCTTCCTAGTAGTTCTGATTTGGATTTACTC
CATAAAATTGTTTTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATAT
CTTTTCCAAGAGCCCCATTTTTGCTGGGGTAGTTCTTCCTCAAGTTCAAC
ACCCCAAAAATGCAAGAAAAAAATATCCAAAGCTTAATGGATTGTTGGCA
GATATAGTTCATGCTTGTTTACAAATTGATCCTGCTGACAGGATATCATC
TAGTGATCTTTTGCATCATGAGTATTTTACTAGAGATGGATTTATTGAAA
AATTCATGCCAGAACTGAAAGCTAAATTACTGCAGGAAGCAAAAGTCAAT
TCATTAATAAAGCCAAAAGAGAGTTCTAAAGAAAATGAACTCAGGAAAGA
TGAAAGAAAAACAGTTTATACCAATACACTGCTAAGTAGTTCAGTTTTGG
GAAAGGAAATAGAAAAAGAGAAAAAGCCCAAGGAGATCAAAGTCAGAGTT
ATTAAAGTCAAAGGAGGAAGAGGAGATATCTCAGAACCAAAAAAGAAAGA
GTATGAAGGTGGACTTGGTCAACAGGATGCAAATGAAAATGTTCATCCTA
TGTCTCCAGATACAAAACTTGTAACCATTGAACCACCAAACCCTATCAAT
CCCAGCACTAACTGTAATGGCTTGAAAGAAAATCCACATTGCGGAGGTTC
TGTGACAATGCCACCCATCAATCTAACTAACAGTAATTTGATGGCTGCAA
ATCTCAGTTCAAATCTCTTTCACCCCAGTGTGAGGTTAACTGAAAGAGCA
AAAAAGAGACGCACTTCTTCACAATCTATTGGACAAGTTATGCCTAATAG
CAGGCAAGAGGATCCAGGTCCTATTCAAAGCCAAATGGAGAAGGGTATAT
TTAATGAGCGAACAGGTCACAGTGACCAAATGGCAAATGAGAACAAAAGG
AAGCTGAATTTTTCCAGATCTGACAGGAAAGAATTCCATTTTCCAGAATT
GCCTGTCACAATACAGTCAAAAGATACAAAAGGAATGGAAGTTAAACAGA
TAAAAATGCTGAAGAGGGAGTCAAAGAAAACAGAGTCATCTAAGATACCA
ACTTTACTTAACGTGGATCAAAATCAAGAAAAACAAGAGGGTGGAGATGG
CCATTGCGAGGGGAAGAATTTGAAGAGAAACAGGTTTTTTTTCTGGTAGG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTEMYETLGKV
GEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHE
NLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQIL
RAIDYLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDY
VATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLL
HKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLA
DIVHACLQIDPADRISSSDLLHHEYFTRDGFIEKFMPELKAKLLQEAKVN
SLIKPKESSKENELRKDERKTVYTNTLLSSSVLGKEIEKEKKPKEIKVRV
IKVKGGRGDISEPKKKEYEGGLGQQDANENVHPMSPDTKLVTIEPPNPIN
PSTNCNGLKENPHCGGSVTMPPINLTNSNLMAANLSSNLFHPSVRLTERA
KKRRTSSQSIGQVMPNSRQEDPGPIQSQMEKGIFNERTGHSDQMANENKR
KLNFSRSDRKEFHFPELPVTIQSKDTKGMEVKQIKMLKRESKKTESSKIP
TLLNVDQNQEKQEGGDGHCEGKNLKRNRFFFW*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4142
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.