cDNA Clone hs PABPC II

• Plasmid: pGEX6P-1-hs PABPC II
• Parent Plasmid: pGEX6P1
• Expressed: GST-hs PABPC II
• Genbank: Z48501/CAA88401
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCTCTACG
AGAAGTTCAGCCCGGCCGGGCCCATCCTCTCCATCCGGGTCTGCAGGGAC
ATGATCACCCGCCGCTCCTTGGGCTACGCGTATGTGAACTTCCAGCAGCC
GGCGGACGCGGAGCGTGCTTTGGACACCATGAATTTTGATGTTATAAAGG
GCAAGCCAGTACGCATCATGTGGTCTCAGCGTGATCCATCACTTCGCAAA
AGTGGAGTAGGCAACATATTCATTAAAAATCTGGACAAATCCATTGATAA
TAAAGCACTGTATGATACATTTTCTGCTTTTGGTAACATCCTTTCATGTA
AGGTGGTTTGTGATGAAAATGGTTCCAAGGGCTATGGATTTGTACACTTT
GAGACGCAGGAAGCAGCTGAAAGAGCTATTGAAAAAATGAATGGAATGCT
CCTAAATGATCGCAAAGTATTTGTTGGACGATTTAAGTCTCGTAAAGAAC
GAGAAGCTGAACTTGGAGCTAGGGCAAAAGAATTCACCAATGTTTACATC
AAGAATTTTGGAGAAGACATGGATGATGAGCGCCTTAAGGATCTCTTTGG
CAAGTTTGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAA
AATCCAAAGGATTTGGATTTGTAAGCTTTGAAAGGCATGAAGATGCACAG
AAAGCTGTGGATGAGATGAACGGAAAGGAGCTCAATGGAAAACAAATTTA
TGTTGGTCGAGCTCAGAAAAAGGTGGAACGGCAGACGGAACTTAAGCGCA
AATTTGAACAGATGAAACAAGATAGGATCACCAGATACCAGGGTGTTAAT
CTTTATGTGAAAAATCTTGATGATGGTATTGATGATGAACGTCTCCGGAA
AGAGTTTTCTCCATTTGGTACAATCACTAGTGCAAAGGTTATGATGGAGG
GTGGTCGCAGCAAAGGGTTTGGTTTTGTATGTTTCTCCTCCCCAGAAGAA
GCCACTAAAGCAGTTACAGAAATGAACGGTAGAATTGTGGCCACAAAGCC
ATTGTATGTAGCTTTAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCA
CTAACCAGTATATGCAGAGAATGGCAAGTGTACGAGCTGTTCCCAACCCT
GTAATCAACCCCTACCAGCCAGCACCTCCTTCAGGTTACTTCATCGCAGC
TATCCCACAGACTCAGAACCGTGCTGCATACTATCCTCCTAGCCAAATTG
CTCAACTAAGACCAAGTCCTCGCTGGACTGCTCAGGGTGCCAGACCTCAT
CCTGCTGTTCATGTACAAGGTCAGGAACCTTTGACTGCTTCCATGTTGGC
ATCTGCCCCTCCTCAAGAGCAAAAGCAAATGTTGGGTGAACGGCTGTTTC
CTCTTATTCAAGCCATGCACCCTACTCTTGCTGGTAAAATCACTGGCATG
TTGTTGGAGATTGATAATTCAGAACTTCTTCATATGCTCGAGTCTCCAGA
GTCACTCCGTTCTAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACC
AAGCTAAAGAGGCTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTTCCA
ACTGTTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLYEKFSPAGPILSIRVCRD
MITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRK
SGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHF
ETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYI
KNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQ
KAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVN
LYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEE
ATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNP
VINPYQPAPPSGYFIAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPH
PAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGM
LLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVP
TV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST