cDNA Clone hs PABPC I

• Synonyms: PAB1; PABP; PABP1; PABPC2; PABPL1
• Plasmid: pGEX6P-1-hs PABPC I
• Parent Plasmid: pGEX6P1
• Expressed: GST-hs PABPC I
• Genbank: NM_002568
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCAACCCCA
GTGCCCCCAGCTACCCCATGGCCTCGCTCTACGTGGGGGACCTCCACCCC
GACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCAT
CCTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCT
ACGCGTATGTGAACTTCCAGCAGCCGGCGGACGCGGAGCGTGCTTTGGAC
ACCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTC
TCAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTA
AAAATCTGGACAAATCCATTGATAATAAAGCACTGTATGATACATTTTCT
GCTTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAATGGTTC
CAAGGGCTATGGATTTGTACACTTTGAGACGCAGGAAGCAGCTGAAAGAG
CTATTGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTGTT
GGACGATTTAAGTCTCGTAAAGAACGAGAAGCTGAACTTGGAGCTAGGGC
AAAAGAATTCACCAATGTTTACATCAAGAATTTTGGAGAAGACATGGATG
ATGAGCGCCTTAAGGATCTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTG
AAAGTAATGACTGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAG
CTTTGAAAGGCATGAAGATGCACAGAAAGCTGTGGATGAGATGAACGGAA
AGGAGCTCAATGGAAAACAAATTTATGTTGGTCGAGCTCAGAAAAAGGTG
GAACGGCAGACGGAACTTAAGCGCAAATTTGAACAGATGAAACAAGATAG
GATCACCAGATACCAGGGTGTTAATCTTTATGTGAAAAATCTTGATGATG
GTATTGATGATGAACGTCTCCGGAAAGAGTTTTCTCCATTTGGTACAATC
ACTAGTGCAAAGGTTATGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTT
TGTATGTTTCTCCTCCCCAGAAGAAGCCACTAAAGCAGTTACAGAAATGA
ACGGTAGAATTGTGGCCACAAAGCCATTGTATGTAGCTTTAGCTCAGCGC
AAAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATGCAGAGAATGGC
AAGTGTACGAGCTGTTCCCAACCCTGTAATCAACCCCTACCAGCCAGCAC
CTCCTTCAGGTTACTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCT
GCATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCAAGTCCTCGCTG
GACTGCTCAGGGTGCCAGACCTCATCCATTCCAAAATATGCCCGGTGCTA
TCCGCCCAGCTGCTCCTAGACCACCATTTAGTACTATGAGACCAGCTTCT
TCACAGGTTCCACGAGTCATGTCAACACAGCGTGTTGCTAACACATCAAC
ACAGACAATGGGTCCACGTCCTGCAGCTGCAGCCGCTGCAGCTACTCCTG
CTGTCCGCACCGTTCCACAGTATAAATATGCTGCAGGAGTTCGCAATCCT
CAGCAACATCTTAATGCACAGCCACAAGTTACAATGCAACAGCCTGCTGT
TCATGTACAAGGTCAGGAACCTTTGACTGCTTCCATGTTGGCATCTGCCC
CTCCTCAAGAGCAAAAGCAAATGTTGGGTGAACGGCTGTTTCCTCTTATT
CAAGCCATGCACCCTACTCTTGCTGGTAAAATCACTGGCATGTTGTTGGA
GATTGATAATTCAGAACTTCTTCATATGCTCGAGTCTCCAGAGTCACTCC
GTTCTAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCAAGCTAAA
GAGGCTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTTCCAACTGTTTA
AGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSNPSAPSYPMASLYVGDLHP
DVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALD
TMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFS
AFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFV
GRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSV
KVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKV
ERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTI
TSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR
KEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRA
AYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPAS
SQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNP
QQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLI
QAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAK
EAAQKAVNSATGVPTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-hs PABPC I111.89 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU4131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.
• Low Temp: No