cDNA Clone DAZAP1-b

• Plasmid: pET28a(+) DAZAP1-b T269A T315A
• Parent Plasmid: pET28a original
• Expressed: His-DAZAP1-b T269A T315A
• Genbank: NM_018959
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTT
GACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGG
AGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTC
GAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTG
CTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCC
ATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGAT
GGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTC
GGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAA
GAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGC
AGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTG
GACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGA
AGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGC
CAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGC
TGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGG
AATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTG
CAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTG
TCCGCCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTA
CGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGC
CAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGCTCCCGGG
GCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACAT
GAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAG
GTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGC
CAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCC
CCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAG
GGTTCCACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSNNSGADEIGKLFVGGL
DWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTV
LASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFV
GGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
SAPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4130
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal 6H tag.