cDNA Clone DAZAP1-b

• Plasmid: pET28a(+) DAZAP1-b T269D T315D
• Parent Plasmid: pET28a original
• Expressed: His-DAZAP1-b T269D T315D
• Genbank: NM_018959
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTT
GACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGG
AGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTC
GAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTG
CTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCC
ATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGAT
GGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTC
GGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAA
GAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGC
AGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTG
GACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGA
AGTTAAACGAGCTGAGCCACGGGACAGCAAGAGCCAAGCGCCGGGACAGC
CAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGC
TGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGG
AATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTG
CAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTG
TCCGACCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTA
CGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGC
CAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGATCCCGGG
GCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACAT
GAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAG
GTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGC
CAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCC
CCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAGG
GGTTCCACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSNNSGADEIGKLFVGGL
DWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTV
LASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFV
GGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
SDPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPADPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4129
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal 6H tag.