cDNA Clone EWSFLI 1

• Plasmid: pEBG6P-1 EWSFLI1
• Parent Plasmid: pEBG6P
• Expressed: GST-EWSFLI1
• Genbank: NP_005234/NM_002017
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATCGATGCGTCCACGGATTACAGTACC
TATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTTACACCGCCCAGCC
CACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATG
GAACCTATGGACAGCCCACTGATGTCAGCTATACCCAGGCTCAGACCACT
GCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCAC
TGGTTATACTACTCCAACTGCCCCCCAGGCATACAGCCAGCCTGTCCAGG
GGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACC
CAGGCCTCCTATGCAGCTCAGTCTGCATATGGCACTCAGCCTGCTTATCC
AGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAGGATG
GAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTAC
AACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGT
ACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACCTCCATCCTACCCTC
CTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTAC
TCTCAGCAGAACACCTATGGGCAACCGAGCAGCTATGGACAGCAGAGTAG
CTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCCCACTAGTTACCCAC
CCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATATAGCCAACAGAGC
AGCAGCTACGGGCAGCAGGACCCTTCTTATGACTCAGTCAGAAGAGGAGC
TTGGGGCAATAACATGAATTCTGGCCTCAACAAAAGTCCTCCCCTTGGAG
GGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCAGATCCG
TATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGG
GCAGATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCA
ACGCCAGCTGTATCACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACG
GACCCCGATGAGGTGGCCAGGCGCTGGGGCGAGCGGAAAAGCAAGCCCAA
CATGAATTACGACAAGCTGAGCCGGGCCCTCCGTTATTACTATGATAAAA
ACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTTGACTTC
CACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTA
CAAGTACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGC
AGAAGGTGAACTTTGTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCC
TCCAGCTTCTTTGGAGCCGCATCACAATACTGGACCTCCCCCACGGGGGG
AATCTACCCCAACCCCAACGTCCCCCGCCATCCTAACACCCACGTGCCTT
CACACTTAGGCAGCTACTACTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDASTDYST
YSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTT
ATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTT
QASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGY
NQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSY
SQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQS
SSYGQQDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDP
YQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMT
DPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDF
HGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTS
SSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.