cDNA Clone EWSFLI 1

• Plasmid: pCMV5-HA EWSFLI 1 T79A
• Parent Plasmid: pCMV5
• Expressed: HA-EWSFLI 1 T79A
• Genbank: NP_005234/NM_002017
• Tag: HA

Sequence of Insert

CAGATCTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCGTCCA
CGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCT
TACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGG
GCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCC
AGGCTCAGACCACTGCAACCTATGGGCAGACCGCTTATGCAACTTCTTAT
GGACAGCCTCCCACTGGTTATACTGCTCCAACTGCCCCCCAGGCATACAG
CCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTA
CAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGGCACT
CAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTAC
AAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTA
GCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTAC
AGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACC
TCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATG
ATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAGCTAT
GGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCC
CACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAAT
ATAGCCAACAGAGCAGCAGCTACGGGCAGCAGGACCCTTCTTATGACTCA
GTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAAAG
TCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGC
CCCAGCCAGATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCC
AACCCTGGAAGCGGGCAGATCCAGCTGTGGCAATTCCTCCTGGAGCTGCT
CTCCGACAGCGCCAACGCCAGCTGTATCACCTGGGAGGGGACCAACGGGG
AGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGCTGGGGCGAGCGG
AAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGTTA
TTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTT
ACAAATTTGACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACC
GAGTCGTCCATGTACAAGTACCCTTCTGACATCTCCTACATGCCTTCCTA
CCATGCCCACCAGCAGAAGGTGAACTTTGTCCCTCCCCATCCATCCTCCA
TGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCACAATACTGGACC
TCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCTAA
CACCCACGTGCCTTCACACTTAGGCAGCTACTACTAGGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSY
GTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTAPTAPQAYSQPVQ
GYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQD
GNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYP
PTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYP
PQTGSYSQAPSQYSQQSSSYGQQDPSYDSVRRGAWGNNMNSGLNKSPPLG
GAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSA
NASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDK
NIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQ
QKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVP
SHLGSYY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4121
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.