cDNA Clone EWSFLI 1

• Plasmid: pCMV5-HA EWSFLI 1
• Parent Plasmid: pCMV5
• Expressed: HA-EWSFLI 1
• Genbank: NP_005234/NM_002017
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCGTCCAC
GGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTACAGTGCTT
ACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGG
CAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGCTATACCCA
GGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATG
GACAGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGGCATACAGC
CAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTAC
AGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATATGGCACTC
AGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACA
AGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCTCAATCTAG
CACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACA
GTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCACTGCACCT
CCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGA
TCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAGCAGCTATG
GACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCCC
ACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCAAGTCAATA
TAGCCAACAGAGCAGCAGCTACGGGCAGCAGGACCCTTCTTATGACTCAG
TCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAAAGT
CCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCC
CCAGCCAGATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCA
ACCCTGGAAGCGGGCAGATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTC
TCCGACAGCGCCAACGCCAGCTGTATCACCTGGGAGGGGACCAACGGGGA
GTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGCTGGGGCGAGCGGA
AAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGTTAT
TACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTA
CAAATTTGACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCG
AGTCGTCCATGTACAAGTACCCTTCTGACATCTCCTACATGCCTTCCTAC
CATGCCCACCAGCAGAAGGTGAACTTTGTCCCTCCCCATCCATCCTCCAT
GCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCACAATACTGGACCT
CCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCTAAC
ACCCACGTGCCTTCACACTTAGGCAGCTACTACTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSY
GTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQ
GYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQD
GNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYP
PTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYP
PQTGSYSQAPSQYSQQSSSYGQQDPSYDSVRRGAWGNNMNSGLNKSPPLG
GAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSA
NASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDK
NIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQ
QKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVP
SHLGSYY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4120
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.