cDNA Clone hs PABPC I

• Plasmid: pEBG6P-1 hs PABPC I
• Parent Plasmid: pEBG6P
• Expressed: GST-hs PABPC I
• Genbank: NM_002568
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAACCCCAGTGCCCCCAGCTACCCCATGGCCTCGCTCTAC
GTGGGGGACCTCCACCCCGACGTGACCGAGGCGATGCTCTACGAGAAGTT
CAGCCCGGCCGGGCCCATCCTCTCCATCCGGGTCTGCAGGGACATGATCA
CCCGCCGCTCCTTGGGCTACGCGTATGTGAACTTCCAGCAGCCGGCGGAC
GCGGAGCGTGCTTTGGACACCATGAATTTTGATGTTATAAAGGGCAAGCC
AGTACGCATCATGTGGTCTCAGCGTGATCCATCACTTCGCAAAAGTGGAG
TAGGCAACATATTCATTAAAAATCTGGACAAATCCATTGATAATAAAGCA
CTGTATGATACATTTTCTGCTTTTGGTAACATCCTTTCATGTAAGGTGGT
TTGTGATGAAAATGGTTCCAAGGGCTATGGATTTGTACACTTTGAGACGC
AGGAAGCAGCTGAAAGAGCTATTGAAAAAATGAATGGAATGCTCCTAAAT
GATCGCAAAGTATTTGTTGGACGATTTAAGTCTCGTAAAGAACGAGAAGC
TGAACTTGGAGCTAGGGCAAAAGAATTCACCAATGTTTACATCAAGAATT
TTGGAGAAGACATGGATGATGAGCGCCTTAAGGATCTCTTTGGCAAGTTT
GGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCAA
AGGATTTGGATTTGTAAGCTTTGAAAGGCATGAAGATGCACAGAAAGCTG
TGGATGAGATGAACGGAAAGGAGCTCAATGGAAAACAAATTTATGTTGGT
CGAGCTCAGAAAAAGGTGGAACGGCAGACGGAACTTAAGCGCAAATTTGA
ACAGATGAAACAAGATAGGATCACCAGATACCAGGGTGTTAATCTTTATG
TGAAAAATCTTGATGATGGTATTGATGATGAACGTCTCCGGAAAGAGTTT
TCTCCATTTGGTACAATCACTAGTGCAAAGGTTATGATGGAGGGTGGTCG
CAGCAAAGGGTTTGGTTTTGTATGTTTCTCCTCCCCAGAAGAAGCCACTA
AAGCAGTTACAGAAATGAACGGTAGAATTGTGGCCACAAAGCCATTGTAT
GTAGCTTTAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAACCA
GTATATGCAGAGAATGGCAAGTGTACGAGCTGTTCCCAACCCTGTAATCA
ACCCCTACCAGCCAGCACCTCCTTCAGGTTACTTCATGGCAGCTATCCCA
CAGACTCAGAACCGTGCTGCATACTATCCTCCTAGCCAAATTGCTCAACT
AAGACCAAGTCCTCGCTGGACTGCTCAGGGTGCCAGACCTCATCCATTCC
AAAATATGCCCGGTGCTATCCGCCCAGCTGCTCCTAGACCACCATTTAGT
ACTATGAGACCAGCTTCTTCACAGGTTCCACGAGTCATGTCAACACAGCG
TGTTGCTAACACATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCAG
CCGCTGCAGCTACTCCTGCTGTCCGCACCGTTCCACAGTATAAATATGCT
GCAGGAGTTCGCAATCCTCAGCAACATCTTAATGCACAGCCACAAGTTAC
AATGCAACAGCCTGCTGTTCATGTACAAGGTCAGGAACCTTTGACTGCTT
CCATGTTGGCATCTGCCCCTCCTCAAGAGCAAAAGCAAATGTTGGGTGAA
CGGCTGTTTCCTCTTATTCAAGCCATGCACCCTACTCTTGCTGGTAAAAT
CACTGGCATGTTGTTGGAGATTGATAATTCAGAACTTCTTCATATGCTCG
AGTCTCCAGAGTCACTCCGTTCTAAGGTTGATGAAGCTGTAGCTGTACTA
CAAGCCCACCAAGCTAAAGAGGCTGCCCAGAAAGCAGTTAACAGTGCCAC
CGGTGTTCCAACTGTTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSNPSAPSYPMASLY
VGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPAD
AERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKA
LYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLN
DRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKF
GPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVG
RAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEF
SPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLY
VALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIP
QTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFS
TMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYA
AGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGE
RLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVL
QAHQAKEAAQKAVNSATGVPTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4117
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.