cDNA Clone MEF-DAZAP1-b

• Plasmid: pEGFP-C1-MEF-DAZAP1-b T269A T315A
• Parent Plasmid: pEGFPC1
• Expressed: GFP-MEF-DAZAP1-b T269A T315A
• Genbank: NP_005911/NM_018959
• Tag: GFP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGACTCAGATCTCGAGCTCAAGCTTCGAAT
TCTGCAGTCGACGGTACCGCGGGCCCGGGATCCGGGAGGAAAAAGATTCA
GATCCAGCGAATCACCGACGAGCGGAACCGACAGGTGACTTTCACCAAGC
GGAAGTTTGGCCTGATGAAGAAGGCGTATGAGCTGAGCGTGCTATGTGAC
TGCGAGATCGCACTCATCATCTTCAACCACTCCAACAAGCTGTTCCAGTA
CGCCAGCACCGACATGGACAAGGTGCTGCTCAAGTACACGGAGTACAATG
AGCCACACGAGAGCCGCACCAACGCCGACATCATCGAGACCCTGAGGAAG
AAGGGCTTCAACGGCTGCGACAGTCCCGAGCCCGACGGGGAGGACTCGCT
GGAACAGAGCCCCCTGCTGGAGGACAAGTACCGACGCGCCAGCGAGGAGC
TCGACGGGCTCTTCCGGCGCTATGGGTCAACTGTCCCGGCCCCCAACTTT
GCCATGCCTGTCACGGTGCCCGTGTCCAATCAGAGCTCACTGCAGTTCAG
CAATCCCAGCGGCTCCCTGGTCACCCCTTCCCTGGTGACATCATCCCTCA
CGGACCCGCGGCTCCTGTCCCCCCAGCAGCCAGCACTACAGAGGAACAGT
GTGTCTCCTGGCCTGCCCCAGCGGCCAGCTAGTGCGGGGGCCATGCTGGG
GGGTGACCTGAACAGTGCTAACGGAGCCTGCCCCAGCCCTGTTGGGCGAG
GTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGGCTGTC
AACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACGAGC
TGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCC
AGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAG
CCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCC
GGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAG
CCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCCGCCCCTCCT
GGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCC
ACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGTTTG
CCCCTCCGGGGGTTCCTCCTCCACCAGCCGCTCCCGGGGCAGCACCTCTG
GCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCC
GACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGG
ACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCT
TCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGG
CAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTCCACCCCT
ACCGACGCTAGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCD
CEIALIIFNHSNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRK
KGFNGCDSPEPDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNF
AMPVTVPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNS
VSPGLPQRPASAGAMLGGDLNSANGACPSPVGRGFGFITFEDEQSVDQAV
NMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAGQ
PPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSAPP
GGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPGAAPL
AFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQPP
SYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU4101
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP tag.