cDNA Clone SAP97 (rat)

• Plasmid: pCMV5-HA SAP97 S158E T176E S399E S410E
• Parent Plasmid: pCMV5
• Expressed: HA-SAP97 S158E T176E S399E S410E
• Genbank: U14950
• Tag: HA
• Species: Rat

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCGGTCCG
GAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATCGGTCGA
AACTAAACCAAACCGAAGACAGACAGCTCAGGAGTTCCATAGAGCGGGTT
ATTAGCATATTTCAGAGCAACCTCTTTCAGGCTTTAATAGACATTCAAGA
ATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATCATTCAA
AGCAGTGTGAACCGGTCCAACCCGGGAATCCTTGGGAGAGTGGCAGCCTT
TCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCCGGCGGCCTTAGCCCTCC
AGTAGAGAAATACCGGTATCAGGACGAAGAGGTGCTGCCTTCAGAGCGGA
TTTCCCCGCAAGTCCCAAATGAGGTGCTGGGTCCGGAGCTGGTTCACGTC
TCGGAGAAGAGCCTGTCAGAGATTGAGAATGTCCACGGGTTTGTCTCTCA
CTCTCATATCGAACCCATAAAGGCAAATCCTCCTCCTGTGCTGGTCAACA
CAGACAGCTTGGAGGAACCAACTTATGTTAATGGTACTGATGCAGATTAT
GAATATGAGGAAATCACACTTGAAAGGGGAAATTCAGGCCTTGGTTTCAG
CATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAAGTATTT
TCATCACCAAAATTATCACGGGAGGAGCAGCTGCCCAGGATGGAAGATTG
CGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGATGTTCGTGATGT
AACCCACAGCAAAGCAGTTGAAGCATTGAAAGAAGCTGGATCGATAGTGC
GATTATATGTGAAAAGGCGGAAGCCAGCCTCAGAAAAAATCATGGAAATA
AAACTTATTAAAGGCCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTGT
TGGAAACCAGCACATTCCTGGGGATAACAGCATCTATGTAACCAAAATAA
TTGAAGGAGGTGCAGCACACAAGGACGGCAAACTTCAGATTGGAGACAAG
CTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAAGTTACTCATGAAGAAGC
AGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAA
AACCAACAAGTATGTATATAAATGATGGCTATGCACCACCCGACATCACT
AATTCTTCTTCTCAGTCTGTTGACAACCATGTTGAGCCGTCTTCATACTT
GGGCCAGACTCCAGCGGAACCAGCCAGATACTCACCCATTTCTAAAGCAG
TGCTTGGAGATGATGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCAT
CGTGGCTCAACGGGACTTGGTTTCAACATTGTGGGAGGCGAAGATGGAGA
AGGGATTTTTATCTCTTTCATCCTTGCTGGGGGACCTGCTGACCTAAGCG
GAGAGCTCAGAAAAGGAGATCGCATTATATCGGTAAACAGTGTTGACCTC
AGAGCTGCAAGTCATGAACAAGCAGCTGCCGCATTAAAAAACGCAGGCCA
AGCTGTCACTATTGTCGCACAGTATCGGCCTGAAGAGTACAGTCGTTTTG
AAGCTAAAATTCACGACTTGCGGGAGCAGATGATGAACAGCAGTGTCAGC
TCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAGCGGTCCCTCTATGTCAG
AGCCCTTTTTGATTACGACAAGACTAAAGACAGTGGCCTTCCCAGTCAAG
GGTTGAACTTCAAATTCGGAGATATTCTCCATGTTATCAATGCTTCTGAT
GACGAGTGGTGGCAGGCCAGACAGGTGACCCCAGATGGTGAGAGTGACGA
AGTTGGAGTAATTCCTAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCC
GATTAAAAACAGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGTCA
TTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTTCCCTTCTA
CAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGACCAGCACG
TAACTTCTAATGCCAGCGATAGCGAAAGTAGTTACCGTGGTCAAGAAGAA
TATGTTTTATCGTATGAGCCGGTGAATCAGCAAGAAGTTAATTATACCCG
ACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAA
TCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGA
CCAAAGCGTGACTATGAGGTGGATGGACGAGATTACCATTTTGTGACTTC
AAGAGAACAGATGGAGAAAGATATCCAGGAGCATAAATTCATTGAAGCTG
GCCAGTATAACAACCATCTATACGGAACGAGTGTCCAATCTGTGCGAGCA
GTAGCAGAGAAGGGCAAGCATTGTATCCTTGATGTGTCTGGAAATGCCAT
CAAGAGGTTGCAGATTGCACAGCTCTATCCAATATCTACTTTTATTAAAC
CCAAATCCATGGAAAATATCATGGAAATGAATAAACGCCTAACAGACGAA
CAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGACTGGAGCAGGAGTTCAC
TGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACA
ATCAAGTGAAGCAGATCATAGAAGAGCAGTCTGGTCCTTACATCTGGGTC
CCAGCAAAAGAAAAATTATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAPVRKQDTQRALHLLEEYRSKLNQTEDRQLRSSIERVISIF
QSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAA
VTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKS
LSEIENVHGFVSHSHIEPIKANPPPVLVNTDSLEEPTYVNGTDADYEYEE
ITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVND
CILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKPASEKIMEIKLIK
GPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAV
NSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSS
QSVDNHVEPSSYLGQTPAEPARYSPISKAVLGDDEITREPRKVVLHRGST
GLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAAS
HEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSG
SLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWW
QARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGSFNDK
RKKNLFSRKFPFYKNKDQSEQETSDADQHVTSNASDSESSYRGQEEYVLS
YEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRD
YEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEK
GKHCILDVSGNAIKRLQIAQLYPISTFIKPKSMENIMEMNKRLTDEQARK
TFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKE
KL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA. NB: Construct is in pCMV5 Not In vector!!